Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg

Detalhes bibliográficos
Autor(a) principal: Marcondes, Jackson [UNESP]
Data de Publicação: 2008
Outros Autores: Hansen, Ekkehard
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1413-86702008000600004
http://hdl.handle.net/11449/3994
Resumo: The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety Vitória de Verão genetically modified.
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spelling Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAgEdible vaccineHepatitis BOral immunizationRecombinant antigenTransgenic plantsThe obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety Vitória de Verão genetically modified.UNESP School of Agricultural Sciences and Veterinary Biochemical Laboratory of Plants and MicroorganismsState University of North Fluminense Laboratory of BiotechnologyUNESP School of Agricultural Sciences and Veterinary Biochemical Laboratory of Plants and MicroorganismsBrazilian Society of Infectious DiseasesUniversidade Estadual Paulista (Unesp)Universidade Estadual do Norte FluminenseMarcondes, Jackson [UNESP]Hansen, Ekkehard2014-05-20T13:17:34Z2014-05-20T13:17:34Z2008-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article469-471application/pdfhttp://dx.doi.org/10.1590/S1413-86702008000600004Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 12, n. 6, p. 469-471, 2008.1413-8670http://hdl.handle.net/11449/399410.1590/S1413-86702008000600004S1413-86702008000600004WOS:000264200300004S1413-86702008000600004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Infectious Diseases2.0830,817info:eu-repo/semantics/openAccess2023-12-18T06:20:21Zoai:repositorio.unesp.br:11449/3994Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:41:50.509312Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
title Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
spellingShingle Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
Marcondes, Jackson [UNESP]
Edible vaccine
Hepatitis B
Oral immunization
Recombinant antigen
Transgenic plants
title_short Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
title_full Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
title_fullStr Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
title_full_unstemmed Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
title_sort Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
author Marcondes, Jackson [UNESP]
author_facet Marcondes, Jackson [UNESP]
Hansen, Ekkehard
author_role author
author2 Hansen, Ekkehard
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual do Norte Fluminense
dc.contributor.author.fl_str_mv Marcondes, Jackson [UNESP]
Hansen, Ekkehard
dc.subject.por.fl_str_mv Edible vaccine
Hepatitis B
Oral immunization
Recombinant antigen
Transgenic plants
topic Edible vaccine
Hepatitis B
Oral immunization
Recombinant antigen
Transgenic plants
description The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety Vitória de Verão genetically modified.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
2014-05-20T13:17:34Z
2014-05-20T13:17:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1413-86702008000600004
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 12, n. 6, p. 469-471, 2008.
1413-8670
http://hdl.handle.net/11449/3994
10.1590/S1413-86702008000600004
S1413-86702008000600004
WOS:000264200300004
S1413-86702008000600004.pdf
url http://dx.doi.org/10.1590/S1413-86702008000600004
http://hdl.handle.net/11449/3994
identifier_str_mv Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 12, n. 6, p. 469-471, 2008.
1413-8670
10.1590/S1413-86702008000600004
S1413-86702008000600004
WOS:000264200300004
S1413-86702008000600004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Infectious Diseases
2.083
0,817
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 469-471
application/pdf
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129235442728960

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