Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.org.ar/scielo.php?pid=S0327-95452008000200004&script=sci_arttext http://hdl.handle.net/11449/2740 |
Resumo: | The spermatogenesis of Pseudis limellum, from the Southern Pantanal, Brazil, was studied from July 1995 to May 1996, through histological sections of the testis. The cells could be differentiated as: primary spermatogonia, large cells, generally with bilobed nucleus; secondary spermatogonia, smaller cells, with darker cytoplasm, chromatin of radial form; primary and secondary spermatocytes, differentiated according to the different stages of the nucleus during the successive cells divisions. Furthermore, we observed cells in process of morphologic differentiation: rounded spermatids much smaller, with nucleus containing chromatin in compacting process and cytoplasm reduction; elongated spermatids, generally parallel organized in well defined bundles, with the anterior region directed toward the periphery of the seminiferous tubule and the tail directed toward the lumen. Spermatozoa are free in the lumen of the seminiferous tubule. All the cells are organized as cysts, which are supported by a large amount of Sertoli cells. The spermatogenesis in P limellum is very similar to that of other anurans, but peculiarities were observed regarding the organization of the germ cells, the great amount of free Sertoli cells in the lumen of testis collected in May, and the long cytoplasmatic extensions of the cells bearing pigments and involving the seminiferous tubule. The diameter of the seminiferous tubule (SD) exhibited an annual mean of 251.79 +/- 37.57 mu m. Spermatozoa number by seminiferous tubule (SN) exhibited an annual mean of 306.66 +/- 39.83, also with higher and lower values at each month. Variations in SD and SN were not significantly correlated with climatic variables. In this species, reproduction Occurs throughout the year in ponds and flooded areas, despite the seasonal climate of the Pantanal. Although males varied in their annual reproductive activity, they were considered potentially reproductive in all months throughout the year. |
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Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazilspermatogenesismaleamphibiaclimatic influencesThe spermatogenesis of Pseudis limellum, from the Southern Pantanal, Brazil, was studied from July 1995 to May 1996, through histological sections of the testis. The cells could be differentiated as: primary spermatogonia, large cells, generally with bilobed nucleus; secondary spermatogonia, smaller cells, with darker cytoplasm, chromatin of radial form; primary and secondary spermatocytes, differentiated according to the different stages of the nucleus during the successive cells divisions. Furthermore, we observed cells in process of morphologic differentiation: rounded spermatids much smaller, with nucleus containing chromatin in compacting process and cytoplasm reduction; elongated spermatids, generally parallel organized in well defined bundles, with the anterior region directed toward the periphery of the seminiferous tubule and the tail directed toward the lumen. Spermatozoa are free in the lumen of the seminiferous tubule. All the cells are organized as cysts, which are supported by a large amount of Sertoli cells. The spermatogenesis in P limellum is very similar to that of other anurans, but peculiarities were observed regarding the organization of the germ cells, the great amount of free Sertoli cells in the lumen of testis collected in May, and the long cytoplasmatic extensions of the cells bearing pigments and involving the seminiferous tubule. The diameter of the seminiferous tubule (SD) exhibited an annual mean of 251.79 +/- 37.57 mu m. Spermatozoa number by seminiferous tubule (SN) exhibited an annual mean of 306.66 +/- 39.83, also with higher and lower values at each month. Variations in SD and SN were not significantly correlated with climatic variables. In this species, reproduction Occurs throughout the year in ponds and flooded areas, despite the seasonal climate of the Pantanal. Although males varied in their annual reproductive activity, they were considered potentially reproductive in all months throughout the year.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratorio de Herpetologia, UNESP, Rio Claro, SPUniv Fed Mato Grosso, Inst Biociencias, Dept Biol & Zool, BR-78040400 Cuiaba, MT, BrazilUniv Estadual Paulista, Univ Estadual Paulista Julio Mesquita Filho, Dept Morfol, São Paulo, BrazilUniv Estadual Paulista, Univ Estadual Paulista Julio Mesquita Filho, Dept Morfol & Fisiol Anim, São Paulo, BrazilUniv Estadual Paulista, Univ Estadual Paulista Julio Mesquita Filho, Dept Morfol, São Paulo, BrazilUniv Estadual Paulista, Univ Estadual Paulista Julio Mesquita Filho, Dept Morfol & Fisiol Anim, São Paulo, BrazilCNPq: 150007/03-5FAPEMAT: 0769/2006FAPESP: 04/00709-0Laboratorio de Herpetologia, UNESP, Rio Claro, SP: 01/13341-3Inst Histol Embriol-conicetUniv Fed Mato GrossoUniversidade Estadual Paulista (Unesp)Ferreira, AdelinaMehanna, Mahmoud [UNESP]Prado, Cynthia P. A. [UNESP]2014-05-20T13:15:40Z2014-05-20T13:15:40Z2008-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article185-194application/pdfhttp://www.scielo.org.ar/scielo.php?pid=S0327-95452008000200004&script=sci_arttextBiocell. Mendoza: Inst Histol Embriol-conicet, v. 32, n. 2, p. 185-194, 2008.0327-9545http://hdl.handle.net/11449/2740S0327-95452008000200004WOS:000259287600004S0327-95452008000200004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiocell0.1180,116info:eu-repo/semantics/openAccess2023-10-09T06:09:50Zoai:repositorio.unesp.br:11449/2740Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:39.883780Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
title |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
spellingShingle |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil Ferreira, Adelina spermatogenesis male amphibia climatic influences |
title_short |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
title_full |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
title_fullStr |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
title_full_unstemmed |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
title_sort |
Morphologic and morphometric analysis of testis of Pseudis limellum (Cope, 1862) (Anura, Hylidae) during the reproductive cycle in the Pantanal, Brazil |
author |
Ferreira, Adelina |
author_facet |
Ferreira, Adelina Mehanna, Mahmoud [UNESP] Prado, Cynthia P. A. [UNESP] |
author_role |
author |
author2 |
Mehanna, Mahmoud [UNESP] Prado, Cynthia P. A. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Univ Fed Mato Grosso Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ferreira, Adelina Mehanna, Mahmoud [UNESP] Prado, Cynthia P. A. [UNESP] |
dc.subject.por.fl_str_mv |
spermatogenesis male amphibia climatic influences |
topic |
spermatogenesis male amphibia climatic influences |
description |
The spermatogenesis of Pseudis limellum, from the Southern Pantanal, Brazil, was studied from July 1995 to May 1996, through histological sections of the testis. The cells could be differentiated as: primary spermatogonia, large cells, generally with bilobed nucleus; secondary spermatogonia, smaller cells, with darker cytoplasm, chromatin of radial form; primary and secondary spermatocytes, differentiated according to the different stages of the nucleus during the successive cells divisions. Furthermore, we observed cells in process of morphologic differentiation: rounded spermatids much smaller, with nucleus containing chromatin in compacting process and cytoplasm reduction; elongated spermatids, generally parallel organized in well defined bundles, with the anterior region directed toward the periphery of the seminiferous tubule and the tail directed toward the lumen. Spermatozoa are free in the lumen of the seminiferous tubule. All the cells are organized as cysts, which are supported by a large amount of Sertoli cells. The spermatogenesis in P limellum is very similar to that of other anurans, but peculiarities were observed regarding the organization of the germ cells, the great amount of free Sertoli cells in the lumen of testis collected in May, and the long cytoplasmatic extensions of the cells bearing pigments and involving the seminiferous tubule. The diameter of the seminiferous tubule (SD) exhibited an annual mean of 251.79 +/- 37.57 mu m. Spermatozoa number by seminiferous tubule (SN) exhibited an annual mean of 306.66 +/- 39.83, also with higher and lower values at each month. Variations in SD and SN were not significantly correlated with climatic variables. In this species, reproduction Occurs throughout the year in ponds and flooded areas, despite the seasonal climate of the Pantanal. Although males varied in their annual reproductive activity, they were considered potentially reproductive in all months throughout the year. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-01 2014-05-20T13:15:40Z 2014-05-20T13:15:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.org.ar/scielo.php?pid=S0327-95452008000200004&script=sci_arttext Biocell. Mendoza: Inst Histol Embriol-conicet, v. 32, n. 2, p. 185-194, 2008. 0327-9545 http://hdl.handle.net/11449/2740 S0327-95452008000200004 WOS:000259287600004 S0327-95452008000200004.pdf |
url |
http://www.scielo.org.ar/scielo.php?pid=S0327-95452008000200004&script=sci_arttext http://hdl.handle.net/11449/2740 |
identifier_str_mv |
Biocell. Mendoza: Inst Histol Embriol-conicet, v. 32, n. 2, p. 185-194, 2008. 0327-9545 S0327-95452008000200004 WOS:000259287600004 S0327-95452008000200004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biocell 0.118 0,116 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
185-194 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Histol Embriol-conicet |
publisher.none.fl_str_mv |
Inst Histol Embriol-conicet |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128367767060480 |