Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4162-9273 http://hdl.handle.net/11449/160029 |
Resumo: | Endometriosis is a degenerative change of the uterine glands and surrounding stroma, characterized by periglandular arrangement of myofibroblasts and deposition of extracellular matrix (ECM). The aim of this study was to evaluate the expression of collagen type I, III and IV and alpha-smooth muscle actin (alpha-SMA) in equine endometriosis, and investigate the role of myofibroblasts in the progression of these processes. A parallel was made with histochemical techniques of Masson's trichrome, Picrosirius Red under polarized light and Periodic Acid-Schiff (PAS). Twenty four uterine biopsies received by the Veterinary Pathology Service and Animal Reproduction of FMVZ, UNESP, Botucatu, SP, were diagnosed with endometriosis. Histological analysis revealed that the orange dense collagen fibers correspond to type I collagen, being prevalent in inactive and inactive destructive endometriosis. The green loose collagen fibers correspond to type III collagen, and are predominant in active and active destructive endometriosis. In the same processes, a greater amount of periglandular myofibroblasts were observed in comparison to inactive and inactive destructive endometriosis. The presence of these cells in active processes are strongly related to an increased deposition of collagen type III in fibrotic nests. Regarding the basement membrane, the active destructive and active endometriosis shows thickening, apparently not related to an increase in expression of type IV collagen. The active destructive and inactive destructive endometriosis exhibited disruption areas in type IV collagen fibers. Thus, it is noted that the myofibroblasts are related to increased deposition of type III collagen in active fibrotic nests. |
id |
UNSP_4af637727f090b8bbf4e72964f0b2bc4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/160029 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosismareendometriosiscollagenmyofibroblastsimmunohistochemistryEndometriosis is a degenerative change of the uterine glands and surrounding stroma, characterized by periglandular arrangement of myofibroblasts and deposition of extracellular matrix (ECM). The aim of this study was to evaluate the expression of collagen type I, III and IV and alpha-smooth muscle actin (alpha-SMA) in equine endometriosis, and investigate the role of myofibroblasts in the progression of these processes. A parallel was made with histochemical techniques of Masson's trichrome, Picrosirius Red under polarized light and Periodic Acid-Schiff (PAS). Twenty four uterine biopsies received by the Veterinary Pathology Service and Animal Reproduction of FMVZ, UNESP, Botucatu, SP, were diagnosed with endometriosis. Histological analysis revealed that the orange dense collagen fibers correspond to type I collagen, being prevalent in inactive and inactive destructive endometriosis. The green loose collagen fibers correspond to type III collagen, and are predominant in active and active destructive endometriosis. In the same processes, a greater amount of periglandular myofibroblasts were observed in comparison to inactive and inactive destructive endometriosis. The presence of these cells in active processes are strongly related to an increased deposition of collagen type III in fibrotic nests. Regarding the basement membrane, the active destructive and active endometriosis shows thickening, apparently not related to an increase in expression of type IV collagen. The active destructive and inactive destructive endometriosis exhibited disruption areas in type IV collagen fibers. Thus, it is noted that the myofibroblasts are related to increased deposition of type III collagen in active fibrotic nests.Univ Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilArquivo Brasileiro Medicina Veterinaria ZootecniaUniversidade Estadual Paulista (Unesp)Costa, L. D. [UNESP]Dias, M. C. [UNESP]Alvarenga, M. A. [UNESP]Sequeira, J. L. [UNESP]2018-11-26T15:46:11Z2018-11-26T15:46:11Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1398-1406application/pdfhttp://dx.doi.org/10.1590/1678-4162-9273Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 69, n. 6, p. 1398-1406, 2017.0102-0935http://hdl.handle.net/11449/16002910.1590/1678-4162-9273S0102-09352017000601398WOS:000422914400006S0102-09352017000601398.pdf3409765424060945Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivo Brasileiro De Medicina Veterinaria E Zootecnia0,248info:eu-repo/semantics/openAccess2023-11-30T06:15:12Zoai:repositorio.unesp.br:11449/160029Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:08:32.942980Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
title |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
spellingShingle |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis Costa, L. D. [UNESP] mare endometriosis collagen myofibroblasts immunohistochemistry |
title_short |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
title_full |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
title_fullStr |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
title_full_unstemmed |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
title_sort |
Identification of collagens types I, III, IV and alpha-SMA and the participation of myofibroblasts in the fibrotic process in equine endometrosis |
author |
Costa, L. D. [UNESP] |
author_facet |
Costa, L. D. [UNESP] Dias, M. C. [UNESP] Alvarenga, M. A. [UNESP] Sequeira, J. L. [UNESP] |
author_role |
author |
author2 |
Dias, M. C. [UNESP] Alvarenga, M. A. [UNESP] Sequeira, J. L. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa, L. D. [UNESP] Dias, M. C. [UNESP] Alvarenga, M. A. [UNESP] Sequeira, J. L. [UNESP] |
dc.subject.por.fl_str_mv |
mare endometriosis collagen myofibroblasts immunohistochemistry |
topic |
mare endometriosis collagen myofibroblasts immunohistochemistry |
description |
Endometriosis is a degenerative change of the uterine glands and surrounding stroma, characterized by periglandular arrangement of myofibroblasts and deposition of extracellular matrix (ECM). The aim of this study was to evaluate the expression of collagen type I, III and IV and alpha-smooth muscle actin (alpha-SMA) in equine endometriosis, and investigate the role of myofibroblasts in the progression of these processes. A parallel was made with histochemical techniques of Masson's trichrome, Picrosirius Red under polarized light and Periodic Acid-Schiff (PAS). Twenty four uterine biopsies received by the Veterinary Pathology Service and Animal Reproduction of FMVZ, UNESP, Botucatu, SP, were diagnosed with endometriosis. Histological analysis revealed that the orange dense collagen fibers correspond to type I collagen, being prevalent in inactive and inactive destructive endometriosis. The green loose collagen fibers correspond to type III collagen, and are predominant in active and active destructive endometriosis. In the same processes, a greater amount of periglandular myofibroblasts were observed in comparison to inactive and inactive destructive endometriosis. The presence of these cells in active processes are strongly related to an increased deposition of collagen type III in fibrotic nests. Regarding the basement membrane, the active destructive and active endometriosis shows thickening, apparently not related to an increase in expression of type IV collagen. The active destructive and inactive destructive endometriosis exhibited disruption areas in type IV collagen fibers. Thus, it is noted that the myofibroblasts are related to increased deposition of type III collagen in active fibrotic nests. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-11-26T15:46:11Z 2018-11-26T15:46:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-9273 Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 69, n. 6, p. 1398-1406, 2017. 0102-0935 http://hdl.handle.net/11449/160029 10.1590/1678-4162-9273 S0102-09352017000601398 WOS:000422914400006 S0102-09352017000601398.pdf 3409765424060945 |
url |
http://dx.doi.org/10.1590/1678-4162-9273 http://hdl.handle.net/11449/160029 |
identifier_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 69, n. 6, p. 1398-1406, 2017. 0102-0935 10.1590/1678-4162-9273 S0102-09352017000601398 WOS:000422914400006 S0102-09352017000601398.pdf 3409765424060945 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 0,248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1398-1406 application/pdf |
dc.publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
publisher.none.fl_str_mv |
Arquivo Brasileiro Medicina Veterinaria Zootecnia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129024138936320 |