Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia?
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502013000300001 http://hdl.handle.net/11449/29019 |
Resumo: | PURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI. |
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Repositório Institucional da UNESP |
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Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia?KidneyIschemiaReperfusionHyperglycemiaPropofolIsofluraneRatsPURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESPUNESPSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Estadual Paulista (Unesp)Carraretto, Antônio RobertoVianna Filho, Pedro Thadeu GalvãoCastiglia, Yara Marcondes Machado [UNESP]Golim, Márjorie de Assis [UNESP]Souza, Aparecida Vitória Gonçalves deCarvalho, Lídia Raquel de [UNESP]Deffune, Elenice [UNESP]Vianna, Pedro Thadeu Galvão [UNESP]2014-05-20T15:14:02Z2014-05-20T15:14:02Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article161-166application/pdfhttp://dx.doi.org/10.1590/S0102-86502013000300001Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 3, p. 161-166, 2013.0102-8650http://hdl.handle.net/11449/2901910.1590/S0102-86502013000300001S0102-86502013000300001WOS:000316126000001S0102-86502013000300001.pdf65078582038994159646764071339214SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirúrgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2024-09-03T14:30:11Zoai:repositorio.unesp.br:11449/29019Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:30:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
spellingShingle |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? Carraretto, Antônio Roberto Kidney Ischemia Reperfusion Hyperglycemia Propofol Isoflurane Rats |
title_short |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_full |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_fullStr |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_full_unstemmed |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
title_sort |
Does propofol and isoflurane protect the kidney against ischemia/reperfusion injury during transient hyperglycemia? |
author |
Carraretto, Antônio Roberto |
author_facet |
Carraretto, Antônio Roberto Vianna Filho, Pedro Thadeu Galvão Castiglia, Yara Marcondes Machado [UNESP] Golim, Márjorie de Assis [UNESP] Souza, Aparecida Vitória Gonçalves de Carvalho, Lídia Raquel de [UNESP] Deffune, Elenice [UNESP] Vianna, Pedro Thadeu Galvão [UNESP] |
author_role |
author |
author2 |
Vianna Filho, Pedro Thadeu Galvão Castiglia, Yara Marcondes Machado [UNESP] Golim, Márjorie de Assis [UNESP] Souza, Aparecida Vitória Gonçalves de Carvalho, Lídia Raquel de [UNESP] Deffune, Elenice [UNESP] Vianna, Pedro Thadeu Galvão [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Carraretto, Antônio Roberto Vianna Filho, Pedro Thadeu Galvão Castiglia, Yara Marcondes Machado [UNESP] Golim, Márjorie de Assis [UNESP] Souza, Aparecida Vitória Gonçalves de Carvalho, Lídia Raquel de [UNESP] Deffune, Elenice [UNESP] Vianna, Pedro Thadeu Galvão [UNESP] |
dc.subject.por.fl_str_mv |
Kidney Ischemia Reperfusion Hyperglycemia Propofol Isoflurane Rats |
topic |
Kidney Ischemia Reperfusion Hyperglycemia Propofol Isoflurane Rats |
description |
PURPOSE: To study the effect of isoflurane (Iso) or propofol (Prop) anesthesia on renal ischemia/reperfusion injury (IRI) during transient hyperglycemia. METHODS: Thirty six rats were randomly assigned into six groups of six animals each: PHS (Sham-Prop=1mg.kg-1.min-1 + Hyperglycemia=2.5g.kg-1 of glucose solution administered intraperitoneally); HIS (Sham-Iso + Hyperglycemia); PHI (Prop + Hyperglycemia + Ischemia); IHI (Iso + Hyperglycemia + Ischemia); PI (Prop + Ischemia), and II (Iso + Ischemia). After 30 minutes of anesthesia induction, right nephrectomy was performed (all animals) and the left renal artery was clamped during 25 minutes (ischemia). The animals were sacrificed after 24 hours and blood collection (to dose creatinine) and left kidney removal were performed for histological analysis, and flow cytometry (FCM): percentage of initial apoptosis (APTi) and viable cells (VC). RESULTS: Serum creatinine (mg/dL) was statistically different in groups PHI (3.60±0.40) and IHI (3.23±1.08), p<0.05. Histological analysis was statistically different in groups PHI (4.0[4.0;5.0]) and IHI (4.5[4.0;5.0]), p<0.05. APTi percentage was statistically different in groups PHI (73.2±7.1), and IHI (48.1±14). VC percentage was statistically different in groups PHI (25.8±6.9) and IHI (38.5±9.2), p<0.05. CONCLUSIONS: Propofol and isoflurane showed the same level of protection against ischemia/reperfusion injury in the normoglycemic groups. Transient hyperglycemia is associated with an increase in IRI. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 2014-05-20T15:14:02Z 2014-05-20T15:14:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502013000300001 Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 3, p. 161-166, 2013. 0102-8650 http://hdl.handle.net/11449/29019 10.1590/S0102-86502013000300001 S0102-86502013000300001 WOS:000316126000001 S0102-86502013000300001.pdf 6507858203899415 9646764071339214 |
url |
http://dx.doi.org/10.1590/S0102-86502013000300001 http://hdl.handle.net/11449/29019 |
identifier_str_mv |
Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 3, p. 161-166, 2013. 0102-8650 10.1590/S0102-86502013000300001 S0102-86502013000300001 WOS:000316126000001 S0102-86502013000300001.pdf 6507858203899415 9646764071339214 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirúrgica Brasileira 0.933 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
161-166 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021384260157440 |