Curcumin reduces inflammation in rat apical periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13819 http://hdl.handle.net/11449/237842 |
Resumo: | Aim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250-280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure-induced apical periodontitis (AP) and rats with pulp exposure-induced apical periodontitis treated with curcumin (AP-CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro-CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF-alpha), interleukin (Il)-6 and Il-1 beta. Tartrate-resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal-Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05. Results Micro-CT revealed no statistically significant differences in bone resorption between the AP and AP-CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF-alpha, Il-6 and Il-1 beta were significantly higher in the periapical lesions of the AP group than in the AP-CUR group (p < .05). The number of TRAP-positive multinucleated cells was higher in the AP group than in the AP-CUR group (p < .05). In OCN-positive cells, no differences were observed between the AP and AP-CUR groups (p > .05). Conclusions Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti-inflammatory effect of curcumin on AP development. |
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Curcumin reduces inflammation in rat apical periodontitisApical periodontitisCurcuminCytokineEndodontic infectionAim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250-280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure-induced apical periodontitis (AP) and rats with pulp exposure-induced apical periodontitis treated with curcumin (AP-CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro-CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF-alpha), interleukin (Il)-6 and Il-1 beta. Tartrate-resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal-Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05. Results Micro-CT revealed no statistically significant differences in bone resorption between the AP and AP-CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF-alpha, Il-6 and Il-1 beta were significantly higher in the periapical lesions of the AP group than in the AP-CUR group (p < .05). The number of TRAP-positive multinucleated cells was higher in the AP group than in the AP-CUR group (p < .05). In OCN-positive cells, no differences were observed between the AP and AP-CUR groups (p > .05). Conclusions Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti-inflammatory effect of curcumin on AP development.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifco e TecnologicoSao Paulo State Univ Unesp, Sch Dent, Endodont Sect, Dept Prevent & Restorat Dent, Sao Paulo, BrazilSao Paulo State Univ Unesp, Sch Dent, Dept Basic Sci, Aracatuba, BrazilSao Paulo State Univ UNESP, Dent Assistance Ctr Disabled Persons CAOE, Sch Dent, Aracatuba, BrazilSao Paulo State Univ Unesp, Sch Dent, Endodont Sect, Dept Prevent & Restorat Dent, Sao Paulo, BrazilSao Paulo State Univ Unesp, Sch Dent, Dept Basic Sci, Aracatuba, BrazilSao Paulo State Univ UNESP, Dent Assistance Ctr Disabled Persons CAOE, Sch Dent, Aracatuba, BrazilFAPESP: 2018/18661-7Conselho Nacional de Desenvolvimento Cientifco e Tecnologico: 315381/2021-3Wiley-BlackwellUniversidade Estadual Paulista (UNESP)Justo, Mariana Pagliusi [UNESP]Cardoso, Carolina de Barros Morais [UNESP]Cantiga-Silva, Cristiane [UNESP]Oliveira, Pedro Henrique Chaves de [UNESP]Sivieri-Araujo, Gustavo [UNESP]Azuma, Mariane Maffei [UNESP]Ervolino, Edilson [UNESP]Cintra, Luciano Tavares Angelo [UNESP]2022-11-30T13:46:24Z2022-11-30T13:46:24Z2022-09-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1111/iej.13819International Endodontic Journal. Hoboken: Wiley, 11 p., 2022.0143-2885http://hdl.handle.net/11449/23784210.1111/iej.13819WOS:000850095900001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2022-11-30T13:46:24Zoai:repositorio.unesp.br:11449/237842Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:25:06.767782Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Curcumin reduces inflammation in rat apical periodontitis |
title |
Curcumin reduces inflammation in rat apical periodontitis |
spellingShingle |
Curcumin reduces inflammation in rat apical periodontitis Justo, Mariana Pagliusi [UNESP] Apical periodontitis Curcumin Cytokine Endodontic infection |
title_short |
Curcumin reduces inflammation in rat apical periodontitis |
title_full |
Curcumin reduces inflammation in rat apical periodontitis |
title_fullStr |
Curcumin reduces inflammation in rat apical periodontitis |
title_full_unstemmed |
Curcumin reduces inflammation in rat apical periodontitis |
title_sort |
Curcumin reduces inflammation in rat apical periodontitis |
author |
Justo, Mariana Pagliusi [UNESP] |
author_facet |
Justo, Mariana Pagliusi [UNESP] Cardoso, Carolina de Barros Morais [UNESP] Cantiga-Silva, Cristiane [UNESP] Oliveira, Pedro Henrique Chaves de [UNESP] Sivieri-Araujo, Gustavo [UNESP] Azuma, Mariane Maffei [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] |
author_role |
author |
author2 |
Cardoso, Carolina de Barros Morais [UNESP] Cantiga-Silva, Cristiane [UNESP] Oliveira, Pedro Henrique Chaves de [UNESP] Sivieri-Araujo, Gustavo [UNESP] Azuma, Mariane Maffei [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Justo, Mariana Pagliusi [UNESP] Cardoso, Carolina de Barros Morais [UNESP] Cantiga-Silva, Cristiane [UNESP] Oliveira, Pedro Henrique Chaves de [UNESP] Sivieri-Araujo, Gustavo [UNESP] Azuma, Mariane Maffei [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] |
dc.subject.por.fl_str_mv |
Apical periodontitis Curcumin Cytokine Endodontic infection |
topic |
Apical periodontitis Curcumin Cytokine Endodontic infection |
description |
Aim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250-280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure-induced apical periodontitis (AP) and rats with pulp exposure-induced apical periodontitis treated with curcumin (AP-CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro-CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF-alpha), interleukin (Il)-6 and Il-1 beta. Tartrate-resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal-Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05. Results Micro-CT revealed no statistically significant differences in bone resorption between the AP and AP-CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF-alpha, Il-6 and Il-1 beta were significantly higher in the periapical lesions of the AP group than in the AP-CUR group (p < .05). The number of TRAP-positive multinucleated cells was higher in the AP group than in the AP-CUR group (p < .05). In OCN-positive cells, no differences were observed between the AP and AP-CUR groups (p > .05). Conclusions Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti-inflammatory effect of curcumin on AP development. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:46:24Z 2022-11-30T13:46:24Z 2022-09-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13819 International Endodontic Journal. Hoboken: Wiley, 11 p., 2022. 0143-2885 http://hdl.handle.net/11449/237842 10.1111/iej.13819 WOS:000850095900001 |
url |
http://dx.doi.org/10.1111/iej.13819 http://hdl.handle.net/11449/237842 |
identifier_str_mv |
International Endodontic Journal. Hoboken: Wiley, 11 p., 2022. 0143-2885 10.1111/iej.13819 WOS:000850095900001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129425583112192 |