Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/fst.37819 http://hdl.handle.net/11449/207555 |
Resumo: | The aim of this study was to investigate the presence of virulence and antimicrobial resistance genes and clonal profile of Enterobacteriaceae isolated from calves, cows, feeding buckets and the milk bucket. A a total of 31 Enterobacteriaceae isolates from calves (6), milk bucket (6), feeding buckets (6) and from cows’ rectum (13) were used. The presence of LT, STa, STb, STx1, STx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge as well as the antimicrobial resistance genes: AmpC MOX, FOX, MIR, ACT, DHA, ACC, CTX-M-1, CTX-M2, TEM KPC and MCR-1 and SH were evaluated by PCR. The LT toxin gene were detected in five isolates (16.1%) and the mrkD gene was detected in three isolates (9.0%). The CTX-M-1 gene was detected in 13 isolates (41.9%), CTX-M-2 in five isolates (16.1%) and ACC-M in four isolates (12.9%). Most of the isolates obtained demonstrated, resistance to cephalothin (87.5%), ampicillin, (87.5%) and streptomycin (84.3%) with multidrug resistance observed in all isolates. Isolates from bucket and cow’s rectum, calves and milk bucket, calf and cow’s rectum shared the same pulsotypes. These findings may suggest that enterobacteria carring virulence and resistance genes may persist in the environment and became a reservoir of these genes. |
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Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genesAntibioticE. coliK. pneumoniaeMilkThe aim of this study was to investigate the presence of virulence and antimicrobial resistance genes and clonal profile of Enterobacteriaceae isolated from calves, cows, feeding buckets and the milk bucket. A a total of 31 Enterobacteriaceae isolates from calves (6), milk bucket (6), feeding buckets (6) and from cows’ rectum (13) were used. The presence of LT, STa, STb, STx1, STx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge as well as the antimicrobial resistance genes: AmpC MOX, FOX, MIR, ACT, DHA, ACC, CTX-M-1, CTX-M2, TEM KPC and MCR-1 and SH were evaluated by PCR. The LT toxin gene were detected in five isolates (16.1%) and the mrkD gene was detected in three isolates (9.0%). The CTX-M-1 gene was detected in 13 isolates (41.9%), CTX-M-2 in five isolates (16.1%) and ACC-M in four isolates (12.9%). Most of the isolates obtained demonstrated, resistance to cephalothin (87.5%), ampicillin, (87.5%) and streptomycin (84.3%) with multidrug resistance observed in all isolates. Isolates from bucket and cow’s rectum, calves and milk bucket, calf and cow’s rectum shared the same pulsotypes. These findings may suggest that enterobacteria carring virulence and resistance genes may persist in the environment and became a reservoir of these genes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-Unesp, Campus de JaboticabalDepartamento de Técnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-Unesp, Campus de JaboticabalDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-Unesp, Campus de JaboticabalDepartamento de Técnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista-Unesp, Campus de JaboticabalFAPESP: 2019/02164-7Universidade Estadual Paulista (Unesp)de ALMEIDA, Camila Chioda [UNESP]Financi, Thaylane Paula [UNESP]Cardozo, Marita Vedovelli [UNESP]Pizauro, Lucas Jose Luduverio [UNESP]Pereira, Natalia [UNESP]Valmorbida, Mylena Karoline [UNESP]Borzi, Mariana Monezi [UNESP]Weiss, Bruno [UNESP]de ÁVILA, Fernando Antônio [UNESP]2021-06-25T10:57:08Z2021-06-25T10:57:08Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article376-380application/pdfhttp://dx.doi.org/10.1590/fst.37819Food Science and Technology (Brazil), v. 41, n. 2, p. 376-380, 2021.1678-457Xhttp://hdl.handle.net/11449/20755510.1590/fst.37819S0101-206120210002003762-s2.0-85103636578S0101-20612021000200376.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Science and Technology (Brazil)info:eu-repo/semantics/openAccess2024-06-07T15:31:09Zoai:repositorio.unesp.br:11449/207555Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:45:21.999963Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
title |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
spellingShingle |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes de ALMEIDA, Camila Chioda [UNESP] Antibiotic E. coli K. pneumoniae Milk |
title_short |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
title_full |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
title_fullStr |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
title_full_unstemmed |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
title_sort |
Enterobacteriaceae in calves, cows and milking environment may act as reservoirs of virulence and antimicrobial resistance genes |
author |
de ALMEIDA, Camila Chioda [UNESP] |
author_facet |
de ALMEIDA, Camila Chioda [UNESP] Financi, Thaylane Paula [UNESP] Cardozo, Marita Vedovelli [UNESP] Pizauro, Lucas Jose Luduverio [UNESP] Pereira, Natalia [UNESP] Valmorbida, Mylena Karoline [UNESP] Borzi, Mariana Monezi [UNESP] Weiss, Bruno [UNESP] de ÁVILA, Fernando Antônio [UNESP] |
author_role |
author |
author2 |
Financi, Thaylane Paula [UNESP] Cardozo, Marita Vedovelli [UNESP] Pizauro, Lucas Jose Luduverio [UNESP] Pereira, Natalia [UNESP] Valmorbida, Mylena Karoline [UNESP] Borzi, Mariana Monezi [UNESP] Weiss, Bruno [UNESP] de ÁVILA, Fernando Antônio [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de ALMEIDA, Camila Chioda [UNESP] Financi, Thaylane Paula [UNESP] Cardozo, Marita Vedovelli [UNESP] Pizauro, Lucas Jose Luduverio [UNESP] Pereira, Natalia [UNESP] Valmorbida, Mylena Karoline [UNESP] Borzi, Mariana Monezi [UNESP] Weiss, Bruno [UNESP] de ÁVILA, Fernando Antônio [UNESP] |
dc.subject.por.fl_str_mv |
Antibiotic E. coli K. pneumoniae Milk |
topic |
Antibiotic E. coli K. pneumoniae Milk |
description |
The aim of this study was to investigate the presence of virulence and antimicrobial resistance genes and clonal profile of Enterobacteriaceae isolated from calves, cows, feeding buckets and the milk bucket. A a total of 31 Enterobacteriaceae isolates from calves (6), milk bucket (6), feeding buckets (6) and from cows’ rectum (13) were used. The presence of LT, STa, STb, STx1, STx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge as well as the antimicrobial resistance genes: AmpC MOX, FOX, MIR, ACT, DHA, ACC, CTX-M-1, CTX-M2, TEM KPC and MCR-1 and SH were evaluated by PCR. The LT toxin gene were detected in five isolates (16.1%) and the mrkD gene was detected in three isolates (9.0%). The CTX-M-1 gene was detected in 13 isolates (41.9%), CTX-M-2 in five isolates (16.1%) and ACC-M in four isolates (12.9%). Most of the isolates obtained demonstrated, resistance to cephalothin (87.5%), ampicillin, (87.5%) and streptomycin (84.3%) with multidrug resistance observed in all isolates. Isolates from bucket and cow’s rectum, calves and milk bucket, calf and cow’s rectum shared the same pulsotypes. These findings may suggest that enterobacteria carring virulence and resistance genes may persist in the environment and became a reservoir of these genes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:57:08Z 2021-06-25T10:57:08Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/fst.37819 Food Science and Technology (Brazil), v. 41, n. 2, p. 376-380, 2021. 1678-457X http://hdl.handle.net/11449/207555 10.1590/fst.37819 S0101-20612021000200376 2-s2.0-85103636578 S0101-20612021000200376.pdf |
url |
http://dx.doi.org/10.1590/fst.37819 http://hdl.handle.net/11449/207555 |
identifier_str_mv |
Food Science and Technology (Brazil), v. 41, n. 2, p. 376-380, 2021. 1678-457X 10.1590/fst.37819 S0101-20612021000200376 2-s2.0-85103636578 S0101-20612021000200376.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Science and Technology (Brazil) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
376-380 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128272115957760 |