Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/193601 |
Resumo: | Objective Lactobacillus reuteri is reported as a beneficial adjunct to conventional periodontitis treatment or as monotherapy for gingivitis in several clinical trials. However, probiotics are not free from the risk of adverse effects. We investigated the effects of live and heat-killed (paraprobiotic) L. reuteri during the development of ligature-induced periodontitis in rats. Methods Thirty-two male rats were divided into four groups—control group: healthy rats, LIP group: rats with ligature induced periodontitis, LIP/PRO group: rats with periodontitis that received live L. reuteri, LIP/PARA group: rats with periodontitis that received heat-killed L. reuteri. Treatment was administered for 30 days prior to ligation and continued for 14 days until euthanasia. The hemimandibles were removed and submitted to radiographic analysis, microcomputerized microtomography (μCT) and histomorphometric analysis. Results Radiographic analysis revealed that pro- and paraprobiotics enhanced the percentage of mesial bone support. The μCT analysis showed that paraprobiotic treatment increased bone volume and trabecular number while decreasing total bone porosity and trabecular separation. Live probiotic led to higher trabecular thickness. In the histomorphometric analysis, paraprobiotics significantly prevented furcation bone loss and collagen breakdown, although both pro- and paraprobiotics similarly reduced mesial bone loss. There were no significant differences in attachment loss or the number of tartrate-resistant acid phosphatase -positive cells across treatments. Conclusions Although viable and heat-killed bacteria reduced mesial alveolar bone loss and ameliorated furcation microarchitecture parameters, paraprobiotics presented the most pronounced results preventing periodontitis development. This can contribute to the future use of paraprobiotics in the prevention and treatment of periodontitis. |
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Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in ratsExperimental periodontitisHost modulation therapyProbioticsObjective Lactobacillus reuteri is reported as a beneficial adjunct to conventional periodontitis treatment or as monotherapy for gingivitis in several clinical trials. However, probiotics are not free from the risk of adverse effects. We investigated the effects of live and heat-killed (paraprobiotic) L. reuteri during the development of ligature-induced periodontitis in rats. Methods Thirty-two male rats were divided into four groups—control group: healthy rats, LIP group: rats with ligature induced periodontitis, LIP/PRO group: rats with periodontitis that received live L. reuteri, LIP/PARA group: rats with periodontitis that received heat-killed L. reuteri. Treatment was administered for 30 days prior to ligation and continued for 14 days until euthanasia. The hemimandibles were removed and submitted to radiographic analysis, microcomputerized microtomography (μCT) and histomorphometric analysis. Results Radiographic analysis revealed that pro- and paraprobiotics enhanced the percentage of mesial bone support. The μCT analysis showed that paraprobiotic treatment increased bone volume and trabecular number while decreasing total bone porosity and trabecular separation. Live probiotic led to higher trabecular thickness. In the histomorphometric analysis, paraprobiotics significantly prevented furcation bone loss and collagen breakdown, although both pro- and paraprobiotics similarly reduced mesial bone loss. There were no significant differences in attachment loss or the number of tartrate-resistant acid phosphatase -positive cells across treatments. Conclusions Although viable and heat-killed bacteria reduced mesial alveolar bone loss and ameliorated furcation microarchitecture parameters, paraprobiotics presented the most pronounced results preventing periodontitis development. This can contribute to the future use of paraprobiotics in the prevention and treatment of periodontitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)PreprintUniversidade Estadual Paulista Julio de Mesquita Filho - UnespFAPESP: 2016/06946-1FAPESP: 2017/26461-5ElsevierUniversidade Estadual Paulista (Unesp)Moraes, Renata M. [UNESP]Lescura, Carlos MatheusMilhan, Noala V.M.Ribeiro, Jaqueline L.Silva, Francielly A.Anbinder, Ana Lia2020-09-28T19:43:17Z2020-09-28T19:43:17Z2020-09-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0003-9969http://hdl.handle.net/11449/19360110.1016/j.archoralbio.2020.1048943873604398448643928885307823443860979679430082730000-0003-3930-42740000-0002-9137-84760000-0002-6398-7880engArchives of Oral Biologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-10-25T06:07:39Zoai:repositorio.unesp.br:11449/193601Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:53:28.568002Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
title |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
spellingShingle |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats Moraes, Renata M. [UNESP] Experimental periodontitis Host modulation therapy Probiotics |
title_short |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
title_full |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
title_fullStr |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
title_full_unstemmed |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
title_sort |
Live and heat-killed Lactobacillus reuteri reduce alveolar bone loss on induced periodontitis in rats |
author |
Moraes, Renata M. [UNESP] |
author_facet |
Moraes, Renata M. [UNESP] Lescura, Carlos Matheus Milhan, Noala V.M. Ribeiro, Jaqueline L. Silva, Francielly A. Anbinder, Ana Lia |
author_role |
author |
author2 |
Lescura, Carlos Matheus Milhan, Noala V.M. Ribeiro, Jaqueline L. Silva, Francielly A. Anbinder, Ana Lia |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moraes, Renata M. [UNESP] Lescura, Carlos Matheus Milhan, Noala V.M. Ribeiro, Jaqueline L. Silva, Francielly A. Anbinder, Ana Lia |
dc.subject.por.fl_str_mv |
Experimental periodontitis Host modulation therapy Probiotics |
topic |
Experimental periodontitis Host modulation therapy Probiotics |
description |
Objective Lactobacillus reuteri is reported as a beneficial adjunct to conventional periodontitis treatment or as monotherapy for gingivitis in several clinical trials. However, probiotics are not free from the risk of adverse effects. We investigated the effects of live and heat-killed (paraprobiotic) L. reuteri during the development of ligature-induced periodontitis in rats. Methods Thirty-two male rats were divided into four groups—control group: healthy rats, LIP group: rats with ligature induced periodontitis, LIP/PRO group: rats with periodontitis that received live L. reuteri, LIP/PARA group: rats with periodontitis that received heat-killed L. reuteri. Treatment was administered for 30 days prior to ligation and continued for 14 days until euthanasia. The hemimandibles were removed and submitted to radiographic analysis, microcomputerized microtomography (μCT) and histomorphometric analysis. Results Radiographic analysis revealed that pro- and paraprobiotics enhanced the percentage of mesial bone support. The μCT analysis showed that paraprobiotic treatment increased bone volume and trabecular number while decreasing total bone porosity and trabecular separation. Live probiotic led to higher trabecular thickness. In the histomorphometric analysis, paraprobiotics significantly prevented furcation bone loss and collagen breakdown, although both pro- and paraprobiotics similarly reduced mesial bone loss. There were no significant differences in attachment loss or the number of tartrate-resistant acid phosphatase -positive cells across treatments. Conclusions Although viable and heat-killed bacteria reduced mesial alveolar bone loss and ameliorated furcation microarchitecture parameters, paraprobiotics presented the most pronounced results preventing periodontitis development. This can contribute to the future use of paraprobiotics in the prevention and treatment of periodontitis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-28T19:43:17Z 2020-09-28T19:43:17Z 2020-09-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
0003-9969 http://hdl.handle.net/11449/193601 10.1016/j.archoralbio.2020.104894 3873604398448643 9288853078234438 6097967943008273 0000-0003-3930-4274 0000-0002-9137-8476 0000-0002-6398-7880 |
identifier_str_mv |
0003-9969 10.1016/j.archoralbio.2020.104894 3873604398448643 9288853078234438 6097967943008273 0000-0003-3930-4274 0000-0002-9137-8476 0000-0002-6398-7880 |
url |
http://hdl.handle.net/11449/193601 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128579445194752 |