Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascibiolsci.v41i1.48785 http://hdl.handle.net/11449/232960 |
Resumo: | Fungi are present in the most diverse environments including the interior of plant tissues, living as endophytes without causing apparent damage. These endophytes are producers of secondary metabolites, also known as natural products, such as fungicides. Here, we evaluated the ethyl acetate fractions obtained from endophytic fungi isolated from plants in the genus Begonia. The fractions were submitted to inhibitory test against the plant pathogens Diaporthe phaseolorum and Colletotrichum gloeosporioides. From the 88 ethyl acetate fractions evaluated, 14.7% inhibited C. gloeosporioides and 11.3% inhibited D. phaseolorum. One fungal isolate displaying an active fraction was selected for chemical investigation. The fungus identified as Neopestalotiopsis sp., produced a compound that was active against D. phaseolorum, with a MIC of 312 jig ml/1 (1,695.3 jiM). The compound was identified by mass spectrometry and 1H NMR as the known compound fumiquinone B. The results highlight that the endophytes are capable of producing compounds that may be used to control plant pathogens. The compound fumiquinone B is reported for the first time as an antifungal agent against D. phaseolorum, a relevant plant pathogen worldwide. This is also the first report of the production of fumiquinone B by the genus Neopestalotiopsis. |
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Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B2,6-hydroxy-5-methoxy-3-methylcyclohexa-25-diene-1,4-dioneDiaporthe phaseolorumNeopestalotiopsis spFungi are present in the most diverse environments including the interior of plant tissues, living as endophytes without causing apparent damage. These endophytes are producers of secondary metabolites, also known as natural products, such as fungicides. Here, we evaluated the ethyl acetate fractions obtained from endophytic fungi isolated from plants in the genus Begonia. The fractions were submitted to inhibitory test against the plant pathogens Diaporthe phaseolorum and Colletotrichum gloeosporioides. From the 88 ethyl acetate fractions evaluated, 14.7% inhibited C. gloeosporioides and 11.3% inhibited D. phaseolorum. One fungal isolate displaying an active fraction was selected for chemical investigation. The fungus identified as Neopestalotiopsis sp., produced a compound that was active against D. phaseolorum, with a MIC of 312 jig ml/1 (1,695.3 jiM). The compound was identified by mass spectrometry and 1H NMR as the known compound fumiquinone B. The results highlight that the endophytes are capable of producing compounds that may be used to control plant pathogens. The compound fumiquinone B is reported for the first time as an antifungal agent against D. phaseolorum, a relevant plant pathogen worldwide. This is also the first report of the production of fumiquinone B by the genus Neopestalotiopsis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Ciências Exatas, Av. Pádua DiasUniversidade Estadual PaulistaChemical Microbiology Helmholtz Centre for Infection ResearchUniversidade Federal de São CarlosUniversidade Estadual PaulistaFAPESP: 2013/50228-8FAPESP: 2014/12021-5FAPESP: 2014/15760-3Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Helmholtz Centre for Infection ResearchUniversidade Federal de São Carlos (UFSCar)Grigoletto, Diana FortkampCorreia, Ana Maria Lima [UNESP]Abraham, Wolf-RainerRodrigues, Andre [UNESP]Assis, Marco Antonio [UNESP]Ferreira, Antonio GilbertoMassaroli, MichelliDe Lira, Simone Possedente2022-04-30T22:28:34Z2022-04-30T22:28:34Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4025/actascibiolsci.v41i1.48785Acta Scientiarum - Biological Sciences, v. 41, n. 1, 2019.1807-863X1679-9283http://hdl.handle.net/11449/23296010.4025/actascibiolsci.v41i1.487852-s2.0-85078407461Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum - Biological Sciencesinfo:eu-repo/semantics/openAccess2022-04-30T22:28:34Zoai:repositorio.unesp.br:11449/232960Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:40:10.098018Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
title |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
spellingShingle |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B Grigoletto, Diana Fortkamp 2,6-hydroxy-5-methoxy-3-methylcyclohexa-2 5-diene-1,4-dione Diaporthe phaseolorum Neopestalotiopsis sp |
title_short |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
title_full |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
title_fullStr |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
title_full_unstemmed |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
title_sort |
Secondary metabolites produced by endophytic fungi: Novel antifungal activity of fumiquinone B |
author |
Grigoletto, Diana Fortkamp |
author_facet |
Grigoletto, Diana Fortkamp Correia, Ana Maria Lima [UNESP] Abraham, Wolf-Rainer Rodrigues, Andre [UNESP] Assis, Marco Antonio [UNESP] Ferreira, Antonio Gilberto Massaroli, Michelli De Lira, Simone Possedente |
author_role |
author |
author2 |
Correia, Ana Maria Lima [UNESP] Abraham, Wolf-Rainer Rodrigues, Andre [UNESP] Assis, Marco Antonio [UNESP] Ferreira, Antonio Gilberto Massaroli, Michelli De Lira, Simone Possedente |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Helmholtz Centre for Infection Research Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Grigoletto, Diana Fortkamp Correia, Ana Maria Lima [UNESP] Abraham, Wolf-Rainer Rodrigues, Andre [UNESP] Assis, Marco Antonio [UNESP] Ferreira, Antonio Gilberto Massaroli, Michelli De Lira, Simone Possedente |
dc.subject.por.fl_str_mv |
2,6-hydroxy-5-methoxy-3-methylcyclohexa-2 5-diene-1,4-dione Diaporthe phaseolorum Neopestalotiopsis sp |
topic |
2,6-hydroxy-5-methoxy-3-methylcyclohexa-2 5-diene-1,4-dione Diaporthe phaseolorum Neopestalotiopsis sp |
description |
Fungi are present in the most diverse environments including the interior of plant tissues, living as endophytes without causing apparent damage. These endophytes are producers of secondary metabolites, also known as natural products, such as fungicides. Here, we evaluated the ethyl acetate fractions obtained from endophytic fungi isolated from plants in the genus Begonia. The fractions were submitted to inhibitory test against the plant pathogens Diaporthe phaseolorum and Colletotrichum gloeosporioides. From the 88 ethyl acetate fractions evaluated, 14.7% inhibited C. gloeosporioides and 11.3% inhibited D. phaseolorum. One fungal isolate displaying an active fraction was selected for chemical investigation. The fungus identified as Neopestalotiopsis sp., produced a compound that was active against D. phaseolorum, with a MIC of 312 jig ml/1 (1,695.3 jiM). The compound was identified by mass spectrometry and 1H NMR as the known compound fumiquinone B. The results highlight that the endophytes are capable of producing compounds that may be used to control plant pathogens. The compound fumiquinone B is reported for the first time as an antifungal agent against D. phaseolorum, a relevant plant pathogen worldwide. This is also the first report of the production of fumiquinone B by the genus Neopestalotiopsis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2022-04-30T22:28:34Z 2022-04-30T22:28:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascibiolsci.v41i1.48785 Acta Scientiarum - Biological Sciences, v. 41, n. 1, 2019. 1807-863X 1679-9283 http://hdl.handle.net/11449/232960 10.4025/actascibiolsci.v41i1.48785 2-s2.0-85078407461 |
url |
http://dx.doi.org/10.4025/actascibiolsci.v41i1.48785 http://hdl.handle.net/11449/232960 |
identifier_str_mv |
Acta Scientiarum - Biological Sciences, v. 41, n. 1, 2019. 1807-863X 1679-9283 10.4025/actascibiolsci.v41i1.48785 2-s2.0-85078407461 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum - Biological Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128685485588480 |