Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-08835-4 http://hdl.handle.net/11449/223705 |
Resumo: | This study compared the effectiveness of GentleWave system (GWS) and passive ultrasonic irrigation (PUI) in removing lipopolysaccharides (LPS) from infected root canals after minimally invasive (MIT) and conventional instrumentation (CIT) techniques. Sixty first premolars with two roots were inoculated with fluorescent LPS conjugate (Alexa Fluor 594). Of those, twelve were dentin pretreated, inoculated with fluorescent LPS conjugate, and submitted to confocal laser scanning microscopy (CLSM) to validate the LPS-infection model. Forty-eight teeth were randomly divided into treatment groups: GWS + MIT, GWS + CIT, PUI + MIT, and PUI + CIT (all, n = 12). Teeth were instrumented with Vortex Blue rotary file size 15/0.04 for MIT and 35/0.04 for CIT. Samples were collected before (s1) and after a root canal procedure (s2) and after cryogenically ground the teeth (s3) for intraradicular LPS analysis. LPS were quantified with LAL assay (KQCL test). GWS + MIT and GWS + CIT were the most effective protocols against LPS, with no difference between them (p > 0.05). PUI + CIT was more effective than PUI + MIT (p < 0.05) but less effective than GWS + MIT and GWS + CIT. GWS was the most effective protocol against LPS in infected root canals using MIT and CIT techniques. |
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spelling |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canalsThis study compared the effectiveness of GentleWave system (GWS) and passive ultrasonic irrigation (PUI) in removing lipopolysaccharides (LPS) from infected root canals after minimally invasive (MIT) and conventional instrumentation (CIT) techniques. Sixty first premolars with two roots were inoculated with fluorescent LPS conjugate (Alexa Fluor 594). Of those, twelve were dentin pretreated, inoculated with fluorescent LPS conjugate, and submitted to confocal laser scanning microscopy (CLSM) to validate the LPS-infection model. Forty-eight teeth were randomly divided into treatment groups: GWS + MIT, GWS + CIT, PUI + MIT, and PUI + CIT (all, n = 12). Teeth were instrumented with Vortex Blue rotary file size 15/0.04 for MIT and 35/0.04 for CIT. Samples were collected before (s1) and after a root canal procedure (s2) and after cryogenically ground the teeth (s3) for intraradicular LPS analysis. LPS were quantified with LAL assay (KQCL test). GWS + MIT and GWS + CIT were the most effective protocols against LPS, with no difference between them (p > 0.05). PUI + CIT was more effective than PUI + MIT (p < 0.05) but less effective than GWS + MIT and GWS + CIT. GWS was the most effective protocol against LPS in infected root canals using MIT and CIT techniques.Division of Endodontics Department of Advanced Oral Sciences and Therapeutics University of Maryland School of DentistryDepartment of Restorative Dentistry Endodontic Division São Paulo State University (Unesp) Institute of Science and TechnologyDepartment of Social and Pediatric Dentistry Institute of Science and Technology São Paulo State University (Unesp) Institute of Science and TechnologyDepartment of Restorative Dentistry Endodontic Division São Paulo State University (Unesp) Institute of Science and TechnologyDepartment of Social and Pediatric Dentistry Institute of Science and Technology São Paulo State University (Unesp) Institute of Science and TechnologySchool of DentistryUniversidade Estadual Paulista (UNESP)Velardi, Johnathan P.Alquria, Theeb A.Alfirdous, Rayyan A.Corazza, Bruna J. M. [UNESP]Gomes, Ana P. M. [UNESP]Silva, Eduardo G. [UNESP]Griffin, Ina L.Tordik, Patricia A.Martinho, Frederico C.2022-04-28T19:52:38Z2022-04-28T19:52:38Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-08835-4Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/22370510.1038/s41598-022-08835-42-s2.0-85126894968Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2022-04-28T19:52:38Zoai:repositorio.unesp.br:11449/223705Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:10:04.128848Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
title |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
spellingShingle |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals Velardi, Johnathan P. |
title_short |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
title_full |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
title_fullStr |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
title_full_unstemmed |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
title_sort |
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals |
author |
Velardi, Johnathan P. |
author_facet |
Velardi, Johnathan P. Alquria, Theeb A. Alfirdous, Rayyan A. Corazza, Bruna J. M. [UNESP] Gomes, Ana P. M. [UNESP] Silva, Eduardo G. [UNESP] Griffin, Ina L. Tordik, Patricia A. Martinho, Frederico C. |
author_role |
author |
author2 |
Alquria, Theeb A. Alfirdous, Rayyan A. Corazza, Bruna J. M. [UNESP] Gomes, Ana P. M. [UNESP] Silva, Eduardo G. [UNESP] Griffin, Ina L. Tordik, Patricia A. Martinho, Frederico C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
School of Dentistry Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Velardi, Johnathan P. Alquria, Theeb A. Alfirdous, Rayyan A. Corazza, Bruna J. M. [UNESP] Gomes, Ana P. M. [UNESP] Silva, Eduardo G. [UNESP] Griffin, Ina L. Tordik, Patricia A. Martinho, Frederico C. |
description |
This study compared the effectiveness of GentleWave system (GWS) and passive ultrasonic irrigation (PUI) in removing lipopolysaccharides (LPS) from infected root canals after minimally invasive (MIT) and conventional instrumentation (CIT) techniques. Sixty first premolars with two roots were inoculated with fluorescent LPS conjugate (Alexa Fluor 594). Of those, twelve were dentin pretreated, inoculated with fluorescent LPS conjugate, and submitted to confocal laser scanning microscopy (CLSM) to validate the LPS-infection model. Forty-eight teeth were randomly divided into treatment groups: GWS + MIT, GWS + CIT, PUI + MIT, and PUI + CIT (all, n = 12). Teeth were instrumented with Vortex Blue rotary file size 15/0.04 for MIT and 35/0.04 for CIT. Samples were collected before (s1) and after a root canal procedure (s2) and after cryogenically ground the teeth (s3) for intraradicular LPS analysis. LPS were quantified with LAL assay (KQCL test). GWS + MIT and GWS + CIT were the most effective protocols against LPS, with no difference between them (p > 0.05). PUI + CIT was more effective than PUI + MIT (p < 0.05) but less effective than GWS + MIT and GWS + CIT. GWS was the most effective protocol against LPS in infected root canals using MIT and CIT techniques. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:38Z 2022-04-28T19:52:38Z 2022-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-08835-4 Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/223705 10.1038/s41598-022-08835-4 2-s2.0-85126894968 |
url |
http://dx.doi.org/10.1038/s41598-022-08835-4 http://hdl.handle.net/11449/223705 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-08835-4 2-s2.0-85126894968 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129292681347072 |