In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572011000200005 http://hdl.handle.net/11449/226337 |
Resumo: | Objective: The purpose of this study was to evaluate the efficacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. Material and Methods: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used: G1) 2.5% sodium hypochlorite (NaOCl); G2) 2% chlorhexidine gel (CLX); G3) pyrogen-free solution. After, these groups were subdivided according to the intracanal medication (ICM): A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2% CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections: a) antimicrobial activity; b) quantification of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn's tests at 5% significance level. Results: The 2.5% NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline. Conclusions: It was concluded that 2.5% NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used. |
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Repositório Institucional da UNESP |
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In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canalsCalcium hydroxideChlorhexidineEndotoxinsPolymyxin BSodium hypochloriteObjective: The purpose of this study was to evaluate the efficacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. Material and Methods: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used: G1) 2.5% sodium hypochlorite (NaOCl); G2) 2% chlorhexidine gel (CLX); G3) pyrogen-free solution. After, these groups were subdivided according to the intracanal medication (ICM): A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2% CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections: a) antimicrobial activity; b) quantification of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn's tests at 5% significance level. Results: The 2.5% NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline. Conclusions: It was concluded that 2.5% NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used.Department of Restorative Dentistry São Paulo State University, São José dos Campos, SPDepartment of Oral Diagnosis and Biosciences São Paulo State University, São José dos Campos, SPDepartment of Restorative Dentistry São Paulo State University, São José dos Campos, SPDepartment of Oral Diagnosis and Biosciences São Paulo State University, São José dos Campos, SPUniversidade Estadual Paulista (UNESP)Maekawa, Lilian Eiko [UNESP]Valera, Marcia Carneiro [UNESP]de Oliveira, Luciane Dias [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Koga-Ito, Cristiane Yumi [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]2022-04-28T22:37:22Z2022-04-28T22:37:22Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article106-111http://dx.doi.org/10.1590/S1678-77572011000200005Journal of Applied Oral Science, v. 19, n. 2, p. 106-111, 2011.1678-77651678-7757http://hdl.handle.net/11449/22633710.1590/S1678-775720110002000052-s2.0-79957853555Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2022-04-28T22:37:22Zoai:repositorio.unesp.br:11449/226337Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:03:47.462385Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
title |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
spellingShingle |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals Maekawa, Lilian Eiko [UNESP] Calcium hydroxide Chlorhexidine Endotoxins Polymyxin B Sodium hypochlorite |
title_short |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
title_full |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
title_fullStr |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
title_full_unstemmed |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
title_sort |
In vitro evaluation of the action of irrigating solutions associated with intracanal medications on Escherichia coli and its endotoxin in root canals |
author |
Maekawa, Lilian Eiko [UNESP] |
author_facet |
Maekawa, Lilian Eiko [UNESP] Valera, Marcia Carneiro [UNESP] de Oliveira, Luciane Dias [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Koga-Ito, Cristiane Yumi [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author_role |
author |
author2 |
Valera, Marcia Carneiro [UNESP] de Oliveira, Luciane Dias [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Koga-Ito, Cristiane Yumi [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Maekawa, Lilian Eiko [UNESP] Valera, Marcia Carneiro [UNESP] de Oliveira, Luciane Dias [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Koga-Ito, Cristiane Yumi [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
dc.subject.por.fl_str_mv |
Calcium hydroxide Chlorhexidine Endotoxins Polymyxin B Sodium hypochlorite |
topic |
Calcium hydroxide Chlorhexidine Endotoxins Polymyxin B Sodium hypochlorite |
description |
Objective: The purpose of this study was to evaluate the efficacy of auxiliary chemical substances and intracanal medications on Escherichia coli and its endotoxin in root canals. Material and Methods: Teeth were contaminated with a suspension of E. coli for 14 days and divided into 3 groups according to the auxiliary chemical substance used: G1) 2.5% sodium hypochlorite (NaOCl); G2) 2% chlorhexidine gel (CLX); G3) pyrogen-free solution. After, these groups were subdivided according to the intracanal medication (ICM): A) Calcium hydroxide paste (Calen®), B) polymyxin B, and C) Calcium hydroxide paste+2% CLX gel. For the control group (G4), pyrogen-free saline solution was used without application of intracanal medication. Samples of the root canal content were collected immediately after biomechanical preparation (BMP), at 7 days after BMP, after 14 days of intracanal medication activity, and 7 days after removal of intracanal medication. The following aspects were evaluated for all collections: a) antimicrobial activity; b) quantification of endotoxin by the Limulus Amebocyte Lysate test (LAL). Results were analyzed by the Kruskal-Wallis and Dunn's tests at 5% significance level. Results: The 2.5% NaOCl and CLX were able to eliminate E. coli from root canal lumen and reduced the amount of endotoxin compared to saline. Conclusions: It was concluded that 2.5% NaOCl and CLX were effective in eliminating E. coli. Only the studied intracanal medications were to reduce the amount of endotoxin present in the root canals, regardless of the irrigant used. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2022-04-28T22:37:22Z 2022-04-28T22:37:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572011000200005 Journal of Applied Oral Science, v. 19, n. 2, p. 106-111, 2011. 1678-7765 1678-7757 http://hdl.handle.net/11449/226337 10.1590/S1678-77572011000200005 2-s2.0-79957853555 |
url |
http://dx.doi.org/10.1590/S1678-77572011000200005 http://hdl.handle.net/11449/226337 |
identifier_str_mv |
Journal of Applied Oral Science, v. 19, n. 2, p. 106-111, 2011. 1678-7765 1678-7757 10.1590/S1678-77572011000200005 2-s2.0-79957853555 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
106-111 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129485463093248 |