Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bioelechem.2022.108177 http://hdl.handle.net/11449/241222 |
Resumo: | The present work describes an easy way to prepare a Chloroplast/PDA@WO3 biohybrid platform based on the deposition of chloroplasts on WO3 substrate previously modified with polydopamine (PDA) film as anchoring agent. The use of PDA as an immobilization matrix for chloroplasts, and also as an electron mediator under LED irradiation, resulted in enhanced photocurrents. The use of the chloroplasts amplified the photocurrent, when compared to the bare substrate (WO3). The best electrode performance was obtained using high intensity LED irradiation at 395 nm, for the electrode exposed for 10 min to 150 μg mL−1 of intact chloroplasts. Amperometric curves obtained by on/off cycles using an applied potential of +0.50 V, in PBS electrolyte (pH 7.0), showed that the presence of 0.2 × 10−3 mol L−1 of simazine caused an approximately 50% decrease of the photobiocurrent. Preliminary studies indicated that the synthesized platform based on intact chloroplasts is a good strategy for studying the behavior of photosynthetic entities, using an LED light-responsive WO3 semiconductor substrate. This work contributes to the understanding of photobiocatalysts that emerge as a new class of materials with sophisticated and intricate structures. These are promising materials with remarkably improved quantum efficiency with potential applications in photobioelectrocatalysis. |
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Repositório Institucional da UNESP |
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spelling |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvementBiohybrid platformIntact chloroplastsMediated electron transferPhotobiocurrent enhancementPhotobioelectrocatalysisThe present work describes an easy way to prepare a Chloroplast/PDA@WO3 biohybrid platform based on the deposition of chloroplasts on WO3 substrate previously modified with polydopamine (PDA) film as anchoring agent. The use of PDA as an immobilization matrix for chloroplasts, and also as an electron mediator under LED irradiation, resulted in enhanced photocurrents. The use of the chloroplasts amplified the photocurrent, when compared to the bare substrate (WO3). The best electrode performance was obtained using high intensity LED irradiation at 395 nm, for the electrode exposed for 10 min to 150 μg mL−1 of intact chloroplasts. Amperometric curves obtained by on/off cycles using an applied potential of +0.50 V, in PBS electrolyte (pH 7.0), showed that the presence of 0.2 × 10−3 mol L−1 of simazine caused an approximately 50% decrease of the photobiocurrent. Preliminary studies indicated that the synthesized platform based on intact chloroplasts is a good strategy for studying the behavior of photosynthetic entities, using an LED light-responsive WO3 semiconductor substrate. This work contributes to the understanding of photobiocatalysts that emerge as a new class of materials with sophisticated and intricate structures. These are promising materials with remarkably improved quantum efficiency with potential applications in photobioelectrocatalysis.São Paulo State University (UNESP) Institute of Chemistry Department of Analytical Physicochemical and Inorganic Chemistry National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactives (INCT-DATREM), Rua Professor Francisco Degni, 55São Paulo State University (UNESP) Institute of Chemistry Department of Biochemistry and Organic Chemistry, Rua Professor Francisco Degni, 55São Paulo State University (UNESP) Institute of Chemistry Department of Analytical Physicochemical and Inorganic Chemistry National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactives (INCT-DATREM), Rua Professor Francisco Degni, 55São Paulo State University (UNESP) Institute of Chemistry Department of Biochemistry and Organic Chemistry, Rua Professor Francisco Degni, 55Universidade Estadual Paulista (UNESP)Silva, Caio César Gonçalves [UNESP]Torquato, Lilian Danielle de Moura [UNESP]de Araújo, Bárbara Camila [UNESP]Mantilla, Hernan Dario Rojas [UNESP]Zanoni, Maria Valnice Boldrin [UNESP]Garrido, Saulo Santesso [UNESP]2023-03-01T20:52:21Z2023-03-01T20:52:21Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bioelechem.2022.108177Bioelectrochemistry, v. 147.1878-562X1567-5394http://hdl.handle.net/11449/24122210.1016/j.bioelechem.2022.1081772-s2.0-85132751585Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioelectrochemistryinfo:eu-repo/semantics/openAccess2023-03-01T20:52:21Zoai:repositorio.unesp.br:11449/241222Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:24.920099Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
title |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
spellingShingle |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement Silva, Caio César Gonçalves [UNESP] Biohybrid platform Intact chloroplasts Mediated electron transfer Photobiocurrent enhancement Photobioelectrocatalysis |
title_short |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
title_full |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
title_fullStr |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
title_full_unstemmed |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
title_sort |
Assessment of WO3 electrode modified with intact chloroplasts as a novel biohybrid platform for photocurrent improvement |
author |
Silva, Caio César Gonçalves [UNESP] |
author_facet |
Silva, Caio César Gonçalves [UNESP] Torquato, Lilian Danielle de Moura [UNESP] de Araújo, Bárbara Camila [UNESP] Mantilla, Hernan Dario Rojas [UNESP] Zanoni, Maria Valnice Boldrin [UNESP] Garrido, Saulo Santesso [UNESP] |
author_role |
author |
author2 |
Torquato, Lilian Danielle de Moura [UNESP] de Araújo, Bárbara Camila [UNESP] Mantilla, Hernan Dario Rojas [UNESP] Zanoni, Maria Valnice Boldrin [UNESP] Garrido, Saulo Santesso [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, Caio César Gonçalves [UNESP] Torquato, Lilian Danielle de Moura [UNESP] de Araújo, Bárbara Camila [UNESP] Mantilla, Hernan Dario Rojas [UNESP] Zanoni, Maria Valnice Boldrin [UNESP] Garrido, Saulo Santesso [UNESP] |
dc.subject.por.fl_str_mv |
Biohybrid platform Intact chloroplasts Mediated electron transfer Photobiocurrent enhancement Photobioelectrocatalysis |
topic |
Biohybrid platform Intact chloroplasts Mediated electron transfer Photobiocurrent enhancement Photobioelectrocatalysis |
description |
The present work describes an easy way to prepare a Chloroplast/PDA@WO3 biohybrid platform based on the deposition of chloroplasts on WO3 substrate previously modified with polydopamine (PDA) film as anchoring agent. The use of PDA as an immobilization matrix for chloroplasts, and also as an electron mediator under LED irradiation, resulted in enhanced photocurrents. The use of the chloroplasts amplified the photocurrent, when compared to the bare substrate (WO3). The best electrode performance was obtained using high intensity LED irradiation at 395 nm, for the electrode exposed for 10 min to 150 μg mL−1 of intact chloroplasts. Amperometric curves obtained by on/off cycles using an applied potential of +0.50 V, in PBS electrolyte (pH 7.0), showed that the presence of 0.2 × 10−3 mol L−1 of simazine caused an approximately 50% decrease of the photobiocurrent. Preliminary studies indicated that the synthesized platform based on intact chloroplasts is a good strategy for studying the behavior of photosynthetic entities, using an LED light-responsive WO3 semiconductor substrate. This work contributes to the understanding of photobiocatalysts that emerge as a new class of materials with sophisticated and intricate structures. These are promising materials with remarkably improved quantum efficiency with potential applications in photobioelectrocatalysis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-03-01T20:52:21Z 2023-03-01T20:52:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bioelechem.2022.108177 Bioelectrochemistry, v. 147. 1878-562X 1567-5394 http://hdl.handle.net/11449/241222 10.1016/j.bioelechem.2022.108177 2-s2.0-85132751585 |
url |
http://dx.doi.org/10.1016/j.bioelechem.2022.108177 http://hdl.handle.net/11449/241222 |
identifier_str_mv |
Bioelectrochemistry, v. 147. 1878-562X 1567-5394 10.1016/j.bioelechem.2022.108177 2-s2.0-85132751585 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioelectrochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129584341712896 |