Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jphs.2021.09.002 http://hdl.handle.net/11449/229821 |
Resumo: | The objective of the present study was to evaluate the action of the crude hydroalcoholic extract of Piper cubeba fruits and isolated lignans (cubebin, dihydrocubebin, ethylcubebin, hinokinin and methylcubebin) on head and neck cancer cells. We evaluated the influence of the Piper cubeba extract and isolated lignans (10, 50 e 100 μg/mL) for 4, 24, 48 and 72 h, in the larynx (Hep-2) and oral (SCC-25) squamous cell carcinoma cells and normal fibroblasts, on morphology, cell proliferation and migration, cytotoxicity, genotoxicity and gene and protein expression (PTGS2, PTGER3, PTGER4, MMP2, MMP9). The results showed that the P. cubeba extract and different lignans do not alter the cellular morphology, but decrease cell proliferation and migration, have low cytotoxic and genotoxic effects, probably due to the alteration of the expression of genes and proteins involved with inflammatory process. From these data, we can conclude that the lignans cubebin and methylcubebin had a greater effect on head and neck cancer cells in the antiproliferative, antimigratory and genotoxic action, and could be the target of the development of new therapies including possible new drugs as a therapeutic resource for the treatment of head and neck cancer due to its immense range of biological properties. |
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Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblastsAnti-inflammatory agentsBioactive compoundsPhytotherapyThe objective of the present study was to evaluate the action of the crude hydroalcoholic extract of Piper cubeba fruits and isolated lignans (cubebin, dihydrocubebin, ethylcubebin, hinokinin and methylcubebin) on head and neck cancer cells. We evaluated the influence of the Piper cubeba extract and isolated lignans (10, 50 e 100 μg/mL) for 4, 24, 48 and 72 h, in the larynx (Hep-2) and oral (SCC-25) squamous cell carcinoma cells and normal fibroblasts, on morphology, cell proliferation and migration, cytotoxicity, genotoxicity and gene and protein expression (PTGS2, PTGER3, PTGER4, MMP2, MMP9). The results showed that the P. cubeba extract and different lignans do not alter the cellular morphology, but decrease cell proliferation and migration, have low cytotoxic and genotoxic effects, probably due to the alteration of the expression of genes and proteins involved with inflammatory process. From these data, we can conclude that the lignans cubebin and methylcubebin had a greater effect on head and neck cancer cells in the antiproliferative, antimigratory and genotoxic action, and could be the target of the development of new therapies including possible new drugs as a therapeutic resource for the treatment of head and neck cancer due to its immense range of biological properties.São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Science (IBILCE) Campus São José do Rio Preto Department of BiologySchool of Medicine of São José do Rio Preto (FAMERP) Department of Molecular BiologyDepartment of Genetics and Evolutive Biology Institute of Biosciences University of São PauloUniversity of Franca (UNIFRAN) Nucleus of Research in Exact and Technological SciencesSão Paulo State University (UNESP) School of Engineering (FEIS) Campus Ilha Solteira Department of Physical ChemistrySão Paulo State University (UNESP) School of Engineering (FEIS) Campus Ilha Solteira Department of Biology and Animal ScienceSão Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Science (IBILCE) Campus São José do Rio Preto Department of BiologySão Paulo State University (UNESP) School of Engineering (FEIS) Campus Ilha Solteira Department of Physical ChemistrySão Paulo State University (UNESP) School of Engineering (FEIS) Campus Ilha Solteira Department of Biology and Animal ScienceUniversidade Estadual Paulista (UNESP)School of Medicine of São José do Rio Preto (FAMERP)Universidade de São Paulo (USP)Nucleus of Research in Exact and Technological SciencesGusson-Zanetoni, Juliana Prado [UNESP]da Silva, Julliene Stephanie Guaraldi Monteiro [UNESP]Picão, Thais Bravo [UNESP]Cardin, Laila Toniol [UNESP]Prates, Janesly [UNESP]Sousa, Stefanie Oliveira [UNESP]Henrique, TiagoOliani, Sonia Maria [UNESP]Tajara, Eloiza Helenae Silva, Marcio Luis AndradeCunha, Nayanne Larissada Silva Gomes, Ana Carolina [UNESP]Laurentiz, Rosangela Silva [UNESP]Rodrigues-Lisoni, Flávia Cristina [UNESP]2022-04-29T08:36:08Z2022-04-29T08:36:08Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article93-102http://dx.doi.org/10.1016/j.jphs.2021.09.002Journal of Pharmacological Sciences, v. 148, n. 1, p. 93-102, 2022.1347-86481347-8613http://hdl.handle.net/11449/22982110.1016/j.jphs.2021.09.0022-s2.0-85118353993Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Pharmacological Sciencesinfo:eu-repo/semantics/openAccess2024-07-10T14:07:29Zoai:repositorio.unesp.br:11449/229821Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:41:11.356906Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
title |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
spellingShingle |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts Gusson-Zanetoni, Juliana Prado [UNESP] Anti-inflammatory agents Bioactive compounds Phytotherapy |
title_short |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
title_full |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
title_fullStr |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
title_full_unstemmed |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
title_sort |
Effect of Piper cubeba total extract and isolated lignans on head and neck cancer cell lines and normal fibroblasts |
author |
Gusson-Zanetoni, Juliana Prado [UNESP] |
author_facet |
Gusson-Zanetoni, Juliana Prado [UNESP] da Silva, Julliene Stephanie Guaraldi Monteiro [UNESP] Picão, Thais Bravo [UNESP] Cardin, Laila Toniol [UNESP] Prates, Janesly [UNESP] Sousa, Stefanie Oliveira [UNESP] Henrique, Tiago Oliani, Sonia Maria [UNESP] Tajara, Eloiza Helena e Silva, Marcio Luis Andrade Cunha, Nayanne Larissa da Silva Gomes, Ana Carolina [UNESP] Laurentiz, Rosangela Silva [UNESP] Rodrigues-Lisoni, Flávia Cristina [UNESP] |
author_role |
author |
author2 |
da Silva, Julliene Stephanie Guaraldi Monteiro [UNESP] Picão, Thais Bravo [UNESP] Cardin, Laila Toniol [UNESP] Prates, Janesly [UNESP] Sousa, Stefanie Oliveira [UNESP] Henrique, Tiago Oliani, Sonia Maria [UNESP] Tajara, Eloiza Helena e Silva, Marcio Luis Andrade Cunha, Nayanne Larissa da Silva Gomes, Ana Carolina [UNESP] Laurentiz, Rosangela Silva [UNESP] Rodrigues-Lisoni, Flávia Cristina [UNESP] |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) School of Medicine of São José do Rio Preto (FAMERP) Universidade de São Paulo (USP) Nucleus of Research in Exact and Technological Sciences |
dc.contributor.author.fl_str_mv |
Gusson-Zanetoni, Juliana Prado [UNESP] da Silva, Julliene Stephanie Guaraldi Monteiro [UNESP] Picão, Thais Bravo [UNESP] Cardin, Laila Toniol [UNESP] Prates, Janesly [UNESP] Sousa, Stefanie Oliveira [UNESP] Henrique, Tiago Oliani, Sonia Maria [UNESP] Tajara, Eloiza Helena e Silva, Marcio Luis Andrade Cunha, Nayanne Larissa da Silva Gomes, Ana Carolina [UNESP] Laurentiz, Rosangela Silva [UNESP] Rodrigues-Lisoni, Flávia Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Anti-inflammatory agents Bioactive compounds Phytotherapy |
topic |
Anti-inflammatory agents Bioactive compounds Phytotherapy |
description |
The objective of the present study was to evaluate the action of the crude hydroalcoholic extract of Piper cubeba fruits and isolated lignans (cubebin, dihydrocubebin, ethylcubebin, hinokinin and methylcubebin) on head and neck cancer cells. We evaluated the influence of the Piper cubeba extract and isolated lignans (10, 50 e 100 μg/mL) for 4, 24, 48 and 72 h, in the larynx (Hep-2) and oral (SCC-25) squamous cell carcinoma cells and normal fibroblasts, on morphology, cell proliferation and migration, cytotoxicity, genotoxicity and gene and protein expression (PTGS2, PTGER3, PTGER4, MMP2, MMP9). The results showed that the P. cubeba extract and different lignans do not alter the cellular morphology, but decrease cell proliferation and migration, have low cytotoxic and genotoxic effects, probably due to the alteration of the expression of genes and proteins involved with inflammatory process. From these data, we can conclude that the lignans cubebin and methylcubebin had a greater effect on head and neck cancer cells in the antiproliferative, antimigratory and genotoxic action, and could be the target of the development of new therapies including possible new drugs as a therapeutic resource for the treatment of head and neck cancer due to its immense range of biological properties. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:36:08Z 2022-04-29T08:36:08Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jphs.2021.09.002 Journal of Pharmacological Sciences, v. 148, n. 1, p. 93-102, 2022. 1347-8648 1347-8613 http://hdl.handle.net/11449/229821 10.1016/j.jphs.2021.09.002 2-s2.0-85118353993 |
url |
http://dx.doi.org/10.1016/j.jphs.2021.09.002 http://hdl.handle.net/11449/229821 |
identifier_str_mv |
Journal of Pharmacological Sciences, v. 148, n. 1, p. 93-102, 2022. 1347-8648 1347-8613 10.1016/j.jphs.2021.09.002 2-s2.0-85118353993 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Pharmacological Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
93-102 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128686824620032 |