Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity

Detalhes bibliográficos
Autor(a) principal: González Rodríguez, Isela I.
Data de Publicação: 2020
Outros Autores: Francisco, Aleff F. [UNESP], Moreira-Dill, Leandro S., Quintero, Aristides, Guimarães, César L.S., Fernandes, Carlos A.H. [UNESP], Takeda, Agnes A.S. [UNESP], Zanchi, Fernando B., Caldeira, Cléopatra A.S., Pereira, Paulo S., Fontes, Marcos R.M. [UNESP], Zuliani, Juliana P., Soares, Andreimar M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.toxcx.2020.100049
http://hdl.handle.net/11449/199043
Resumo: A bioactive compound isolated from the stem extract of Aristolochia sprucei through High Performance Liquid Chromatography (HPLC) was identified via Nuclear Magnetic Resonance (NMR) as the aristolochic acid (AA). This compound showed an inhibitory effect over the myotoxic activity of Bothrops jararacussu and Bothrops asper venoms, being also effective against the indirect hemolytic activity of B. asper venom. Besides, AA also inhibited the myotoxic activity of BthTX-I and MTX-II with an efficiency greater than 60% against both myotoxins. Docking predictions revealed an interesting mechanism, through which the AA displays an interaction profile consistent with its inhibiting abilities, binding to both active and putative sites of svPLA2. Overall, the present findings indicate that AA may bind to critical regions of myotoxic Asp 49 and Lys49-PLA2s from snake venoms, highlighting the relevance of domains comprising the active and putative sites to inhibit these toxins.
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spelling Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activityAntivenom medicinal plantsAristolochia spruceiAristolochic acidMyotoxin inhibitorPhospholipases A2Snake venomsA bioactive compound isolated from the stem extract of Aristolochia sprucei through High Performance Liquid Chromatography (HPLC) was identified via Nuclear Magnetic Resonance (NMR) as the aristolochic acid (AA). This compound showed an inhibitory effect over the myotoxic activity of Bothrops jararacussu and Bothrops asper venoms, being also effective against the indirect hemolytic activity of B. asper venom. Besides, AA also inhibited the myotoxic activity of BthTX-I and MTX-II with an efficiency greater than 60% against both myotoxins. Docking predictions revealed an interesting mechanism, through which the AA displays an interaction profile consistent with its inhibiting abilities, binding to both active and putative sites of svPLA2. Overall, the present findings indicate that AA may bind to critical regions of myotoxic Asp 49 and Lys49-PLA2s from snake venoms, highlighting the relevance of domains comprising the active and putative sites to inhibit these toxins.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Secretaría Nacional de Ciencia, Tecnología e InnovaciónFaculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP Universidade de São Paulo USPLaboratório de Biotecnologia de Proteínas e Compostos Bioativos da Amazônia Ocidental LaBioProt Centro de Estudos de Biomoléculas Aplicadas à Saúde CEBio Fundação Oswaldo Cruz FIOCRUZ Unidade Rondônia e Universidade Federal de Rondônia UNIRCentro de Informaciones e Investigaciones Toxicológicas y Químicas Aplicadas (CEIITOXQUIA) and Departamento de Química FCNYE Universidad Autónoma de Chiriquí UNACHIInstituto Brasileiro Do Meio Ambiente e Dos Recursos Naturais Renováveis IBAMAUnidade de Biotecnologia Universidade de Ribeirão Preto UNAERPInstituto Federal de Goiás IFGDepartamento de Física e Biofísica Instituto de Biociências Universidade Estadual Paulista UNESPLaboratório de Imunologia Celular Aplicada a Saúde Fundação Oswaldo Cruz FIOCRUZ Unidade RondôniaCentro Universitário São Lucas UniSLInstituto Nacional de Ciência e Tecnologia Em Epidemiologia da Amazônia Ocidental INCT – EpiAmODepartamento de Física e Biofísica Instituto de Biociências Universidade Estadual Paulista UNESPUniversidade de São Paulo (USP)UNIRUNACHIIBAMAUNAERPIFGUniversidade Estadual Paulista (Unesp)Unidade RondôniaUniSLINCT – EpiAmOGonzález Rodríguez, Isela I.Francisco, Aleff F. [UNESP]Moreira-Dill, Leandro S.Quintero, AristidesGuimarães, César L.S.Fernandes, Carlos A.H. [UNESP]Takeda, Agnes A.S. [UNESP]Zanchi, Fernando B.Caldeira, Cléopatra A.S.Pereira, Paulo S.Fontes, Marcos R.M. [UNESP]Zuliani, Juliana P.Soares, Andreimar M.2020-12-12T01:29:12Z2020-12-12T01:29:12Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.toxcx.2020.100049Toxicon: X, v. 7.2590-1710http://hdl.handle.net/11449/19904310.1016/j.toxcx.2020.1000492-s2.0-85087048581Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicon: Xinfo:eu-repo/semantics/openAccess2021-10-23T01:35:55Zoai:repositorio.unesp.br:11449/199043Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:16:39.555483Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
title Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
spellingShingle Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
González Rodríguez, Isela I.
Antivenom medicinal plants
Aristolochia sprucei
Aristolochic acid
Myotoxin inhibitor
Phospholipases A2
Snake venoms
title_short Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
title_full Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
title_fullStr Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
title_full_unstemmed Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
title_sort Isolation and structural characterization of bioactive compound from Aristolochia sprucei aqueous extract with anti-myotoxic activity
author González Rodríguez, Isela I.
author_facet González Rodríguez, Isela I.
Francisco, Aleff F. [UNESP]
Moreira-Dill, Leandro S.
Quintero, Aristides
Guimarães, César L.S.
Fernandes, Carlos A.H. [UNESP]
Takeda, Agnes A.S. [UNESP]
Zanchi, Fernando B.
Caldeira, Cléopatra A.S.
Pereira, Paulo S.
Fontes, Marcos R.M. [UNESP]
Zuliani, Juliana P.
Soares, Andreimar M.
author_role author
author2 Francisco, Aleff F. [UNESP]
Moreira-Dill, Leandro S.
Quintero, Aristides
Guimarães, César L.S.
Fernandes, Carlos A.H. [UNESP]
Takeda, Agnes A.S. [UNESP]
Zanchi, Fernando B.
Caldeira, Cléopatra A.S.
Pereira, Paulo S.
Fontes, Marcos R.M. [UNESP]
Zuliani, Juliana P.
Soares, Andreimar M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
UNIR
UNACHI
IBAMA
UNAERP
IFG
Universidade Estadual Paulista (Unesp)
Unidade Rondônia
UniSL
INCT – EpiAmO
dc.contributor.author.fl_str_mv González Rodríguez, Isela I.
Francisco, Aleff F. [UNESP]
Moreira-Dill, Leandro S.
Quintero, Aristides
Guimarães, César L.S.
Fernandes, Carlos A.H. [UNESP]
Takeda, Agnes A.S. [UNESP]
Zanchi, Fernando B.
Caldeira, Cléopatra A.S.
Pereira, Paulo S.
Fontes, Marcos R.M. [UNESP]
Zuliani, Juliana P.
Soares, Andreimar M.
dc.subject.por.fl_str_mv Antivenom medicinal plants
Aristolochia sprucei
Aristolochic acid
Myotoxin inhibitor
Phospholipases A2
Snake venoms
topic Antivenom medicinal plants
Aristolochia sprucei
Aristolochic acid
Myotoxin inhibitor
Phospholipases A2
Snake venoms
description A bioactive compound isolated from the stem extract of Aristolochia sprucei through High Performance Liquid Chromatography (HPLC) was identified via Nuclear Magnetic Resonance (NMR) as the aristolochic acid (AA). This compound showed an inhibitory effect over the myotoxic activity of Bothrops jararacussu and Bothrops asper venoms, being also effective against the indirect hemolytic activity of B. asper venom. Besides, AA also inhibited the myotoxic activity of BthTX-I and MTX-II with an efficiency greater than 60% against both myotoxins. Docking predictions revealed an interesting mechanism, through which the AA displays an interaction profile consistent with its inhibiting abilities, binding to both active and putative sites of svPLA2. Overall, the present findings indicate that AA may bind to critical regions of myotoxic Asp 49 and Lys49-PLA2s from snake venoms, highlighting the relevance of domains comprising the active and putative sites to inhibit these toxins.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:29:12Z
2020-12-12T01:29:12Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.toxcx.2020.100049
Toxicon: X, v. 7.
2590-1710
http://hdl.handle.net/11449/199043
10.1016/j.toxcx.2020.100049
2-s2.0-85087048581
url http://dx.doi.org/10.1016/j.toxcx.2020.100049
http://hdl.handle.net/11449/199043
identifier_str_mv Toxicon: X, v. 7.
2590-1710
10.1016/j.toxcx.2020.100049
2-s2.0-85087048581
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicon: X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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