Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1574-6968.2010.02047.x http://hdl.handle.net/11449/21493 |
Resumo: | Xanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. To initiate the characterization of essential biological processes of Xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, we show that the disruption of the alpha-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its pathogenesis while abolishing completely the ability of Xac to degrade starch. Furthermore, our GFP expression system was used to characterize ORF XAC3408, a hypothetical protein encoded by Xac that shares significant homology to the FtsZ-stabilizing factor ZapA from Bacillus subtilis (ZapABsu). GFP-XAC3408 expressed in Xac exhibited a septal localization pattern typical of GFP-ZapABsu, which indicates that XAC3408 is the Xac orthologue of the cell division protein ZapABsu. The results demonstrate the potential of GFP labeling for protein functional characterizations in Xac, and, in addition, the Xac mutant strain labeled at the septum constitutes a biological model for the exploration of antibacterial compounds able to inhibit cell division in this plant pathogen. |
id |
UNSP_5aa3d4a39a6ffd799cd201d6ce7ae3d6 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/21493 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septumcitrus cankercell divisionalpha-amylaseXanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. To initiate the characterization of essential biological processes of Xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, we show that the disruption of the alpha-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its pathogenesis while abolishing completely the ability of Xac to degrade starch. Furthermore, our GFP expression system was used to characterize ORF XAC3408, a hypothetical protein encoded by Xac that shares significant homology to the FtsZ-stabilizing factor ZapA from Bacillus subtilis (ZapABsu). GFP-XAC3408 expressed in Xac exhibited a septal localization pattern typical of GFP-ZapABsu, which indicates that XAC3408 is the Xac orthologue of the cell division protein ZapABsu. The results demonstrate the potential of GFP labeling for protein functional characterizations in Xac, and, in addition, the Xac mutant strain labeled at the septum constitutes a biological model for the exploration of antibacterial compounds able to inhibit cell division in this plant pathogen.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Ctr Estudos Insetos Sociais, Inst Biociencias, Rio Claro, SP, BrazilFundo Defesa Citricultura, Araraquara, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Recursos Genet & Biotecnol, Brasilia, DF, BrazilUniv Estadual Paulista, Dept Biol, Sao Jose do Rio Preto, BrazilUniv Estadual Paulista, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Ctr Estudos Insetos Sociais, Inst Biociencias, Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Biol, Sao Jose do Rio Preto, BrazilFAPESP: 06/59494-9FAPESP: 04/09173-6Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Fundo Defesa CitriculturaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Martins, Paula M. M. [UNESP]Lau, Ivy F. [UNESP]Bacci Junior, Mauricio [UNESP]Belasque, Josedo Amaral, Alexandre M.Taboga, Sebastiao R. [UNESP]Ferreira, Henrique [UNESP]2014-05-20T14:00:51Z2014-05-20T14:00:51Z2010-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article76-83http://dx.doi.org/10.1111/j.1574-6968.2010.02047.xFems Microbiology Letters. Malden: Wiley-blackwell, v. 310, n. 1, p. 76-83, 2010.0378-1097http://hdl.handle.net/11449/2149310.1111/j.1574-6968.2010.02047.xWOS:00028063320001137763455738642680000-0002-0970-4288Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEMS Microbiology Letters1.7350,790info:eu-repo/semantics/openAccess2024-06-24T13:08:25Zoai:repositorio.unesp.br:11449/21493Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:19:54.708509Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
title |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
spellingShingle |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum Martins, Paula M. M. [UNESP] citrus canker cell division alpha-amylase |
title_short |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
title_full |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
title_fullStr |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
title_full_unstemmed |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
title_sort |
Subcellular localization of proteins labeled with GFP in Xanthomonas citri ssp citri: targeting the division septum |
author |
Martins, Paula M. M. [UNESP] |
author_facet |
Martins, Paula M. M. [UNESP] Lau, Ivy F. [UNESP] Bacci Junior, Mauricio [UNESP] Belasque, Jose do Amaral, Alexandre M. Taboga, Sebastiao R. [UNESP] Ferreira, Henrique [UNESP] |
author_role |
author |
author2 |
Lau, Ivy F. [UNESP] Bacci Junior, Mauricio [UNESP] Belasque, Jose do Amaral, Alexandre M. Taboga, Sebastiao R. [UNESP] Ferreira, Henrique [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fundo Defesa Citricultura Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Martins, Paula M. M. [UNESP] Lau, Ivy F. [UNESP] Bacci Junior, Mauricio [UNESP] Belasque, Jose do Amaral, Alexandre M. Taboga, Sebastiao R. [UNESP] Ferreira, Henrique [UNESP] |
dc.subject.por.fl_str_mv |
citrus canker cell division alpha-amylase |
topic |
citrus canker cell division alpha-amylase |
description |
Xanthomonas citri ssp. citri (Xac) is the causal agent of citrus canker, an economically important disease that affects citrus worldwide. To initiate the characterization of essential biological processes of Xac, we constructed integrative plasmids for the ectopic expression of green fluorescent protein (GFP)-labeled proteins within this bacterium. Here, we show that the disruption of the alpha-amylase gene (amy), the site of plasmid integration into the bacterial chromosome, does not alter its pathogenesis while abolishing completely the ability of Xac to degrade starch. Furthermore, our GFP expression system was used to characterize ORF XAC3408, a hypothetical protein encoded by Xac that shares significant homology to the FtsZ-stabilizing factor ZapA from Bacillus subtilis (ZapABsu). GFP-XAC3408 expressed in Xac exhibited a septal localization pattern typical of GFP-ZapABsu, which indicates that XAC3408 is the Xac orthologue of the cell division protein ZapABsu. The results demonstrate the potential of GFP labeling for protein functional characterizations in Xac, and, in addition, the Xac mutant strain labeled at the septum constitutes a biological model for the exploration of antibacterial compounds able to inhibit cell division in this plant pathogen. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-01 2014-05-20T14:00:51Z 2014-05-20T14:00:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1574-6968.2010.02047.x Fems Microbiology Letters. Malden: Wiley-blackwell, v. 310, n. 1, p. 76-83, 2010. 0378-1097 http://hdl.handle.net/11449/21493 10.1111/j.1574-6968.2010.02047.x WOS:000280633200011 3776345573864268 0000-0002-0970-4288 |
url |
http://dx.doi.org/10.1111/j.1574-6968.2010.02047.x http://hdl.handle.net/11449/21493 |
identifier_str_mv |
Fems Microbiology Letters. Malden: Wiley-blackwell, v. 310, n. 1, p. 76-83, 2010. 0378-1097 10.1111/j.1574-6968.2010.02047.x WOS:000280633200011 3776345573864268 0000-0002-0970-4288 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FEMS Microbiology Letters 1.735 0,790 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
76-83 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129508241309696 |