Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration

Detalhes bibliográficos
Autor(a) principal: De Castro, Mateus Vidigal
Data de Publicação: 2016
Outros Autores: Barbizan, Roberta, Ferreira, Rui Seabra [UNESP], Barraviera, Benedito [UNESP], De Oliveira, Alexandre Leite Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2016/2932784
http://hdl.handle.net/11449/173446
Resumo: Axonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from human blood and another derived from animal blood and Crotalus durissus terrificus venom, as a promising treatment for this type of injury. Lewis rats were submitted to VRA (L4-L6) and had the avulsed roots reimplanted to the surface of the spinal cord, with the aid of fibrin sealant. The spinal cords were processed to evaluate neuronal survival, synaptic stability, and glial reactivity, 4 and 12 weeks after lesion. Sciatic nerves were processed to investigate Schwann cell activity by p 75 NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). Walking track test was used to evaluate gait recovery, up to 12 weeks. The results indicate that both fibrin sealants are similarly efficient. However, the snake-derived fibrin glue is a potentially safer alternative for being a biological and biodegradable product which does not contain human blood derivatives. Therefore, the venom glue can be a useful tool for the scientific community due to its advantages and variety of applications.
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spelling Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and RegenerationAxonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from human blood and another derived from animal blood and Crotalus durissus terrificus venom, as a promising treatment for this type of injury. Lewis rats were submitted to VRA (L4-L6) and had the avulsed roots reimplanted to the surface of the spinal cord, with the aid of fibrin sealant. The spinal cords were processed to evaluate neuronal survival, synaptic stability, and glial reactivity, 4 and 12 weeks after lesion. Sciatic nerves were processed to investigate Schwann cell activity by p 75 NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). Walking track test was used to evaluate gait recovery, up to 12 weeks. The results indicate that both fibrin sealants are similarly efficient. However, the snake-derived fibrin glue is a potentially safer alternative for being a biological and biodegradable product which does not contain human blood derivatives. Therefore, the venom glue can be a useful tool for the scientific community due to its advantages and variety of applications.Department of Structural and Functional Biology Institute of Biology University of CampinasSchool of Medicine at Mucuri (FAMMUC) Federal University of Jequitinhonha and Mucuri Valleys (UFVJM)Department of Tropical Diseases Botucatu Medical School São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Department of Tropical Diseases Botucatu Medical School São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Universidade Estadual de Campinas (UNICAMP)Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)De Castro, Mateus VidigalBarbizan, RobertaFerreira, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]De Oliveira, Alexandre Leite Rodrigues2018-12-11T17:05:31Z2018-12-11T17:05:31Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/2932784Neural Plasticity, v. 2016.1687-54432090-5904http://hdl.handle.net/11449/17344610.1155/2016/29327842-s2.0-849859607302-s2.0-84985960730.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeural Plasticity1,348info:eu-repo/semantics/openAccess2024-08-15T15:22:48Zoai:repositorio.unesp.br:11449/173446Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:22:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
title Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
spellingShingle Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
De Castro, Mateus Vidigal
title_short Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
title_full Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
title_fullStr Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
title_full_unstemmed Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
title_sort Direct Spinal Ventral Root Repair following Avulsion: Effectiveness of a New Heterologous Fibrin Sealant on Motoneuron Survival and Regeneration
author De Castro, Mateus Vidigal
author_facet De Castro, Mateus Vidigal
Barbizan, Roberta
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
De Oliveira, Alexandre Leite Rodrigues
author_role author
author2 Barbizan, Roberta
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
De Oliveira, Alexandre Leite Rodrigues
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv De Castro, Mateus Vidigal
Barbizan, Roberta
Ferreira, Rui Seabra [UNESP]
Barraviera, Benedito [UNESP]
De Oliveira, Alexandre Leite Rodrigues
description Axonal injuries at the interface between central and peripheral nervous system, such as ventral root avulsion (VRA), induce important degenerative processes, mostly resulting in neuronal and motor function loss. In the present work, we have compared two different fibrin sealants, one derived from human blood and another derived from animal blood and Crotalus durissus terrificus venom, as a promising treatment for this type of injury. Lewis rats were submitted to VRA (L4-L6) and had the avulsed roots reimplanted to the surface of the spinal cord, with the aid of fibrin sealant. The spinal cords were processed to evaluate neuronal survival, synaptic stability, and glial reactivity, 4 and 12 weeks after lesion. Sciatic nerves were processed to investigate Schwann cell activity by p 75 NTR expression (4 weeks after surgery) and to count myelinated axons and morphometric evaluation (12 weeks after surgery). Walking track test was used to evaluate gait recovery, up to 12 weeks. The results indicate that both fibrin sealants are similarly efficient. However, the snake-derived fibrin glue is a potentially safer alternative for being a biological and biodegradable product which does not contain human blood derivatives. Therefore, the venom glue can be a useful tool for the scientific community due to its advantages and variety of applications.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2018-12-11T17:05:31Z
2018-12-11T17:05:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2016/2932784
Neural Plasticity, v. 2016.
1687-5443
2090-5904
http://hdl.handle.net/11449/173446
10.1155/2016/2932784
2-s2.0-84985960730
2-s2.0-84985960730.pdf
url http://dx.doi.org/10.1155/2016/2932784
http://hdl.handle.net/11449/173446
identifier_str_mv Neural Plasticity, v. 2016.
1687-5443
2090-5904
10.1155/2016/2932784
2-s2.0-84985960730
2-s2.0-84985960730.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neural Plasticity
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dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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