Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/bioengineering9110694 http://hdl.handle.net/11449/246948 |
Resumo: | Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment. |
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Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactorbioreactorextremophilicoxygen transferenceproteinaseproteolyticpsychrophilicMicrobial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.Departamento de Farmácia Programa de Pós-Graduação em Ciências Farmacêuticas Universidade Estadual da Paraíba (UEPB)Departamento de Tecnologia Bioquímico-Farmacêutica Universidade de São Paulo (USP)Faculdade de Biomedicina Instituto de Ciências Biológicas Universidade Federal do Pará (UFPA)Departamento de Medicina e Enfermagem Universidade Federal de Viçosa (UFV)Centro de Investigación en Biodiversidad para la Salud Universidad Privada Norbert WienerInstituto de Ingeniería Ambiental Química y Biotecnología Aplicada (INGEBIO) Facultad de Química e Ingeniería del Rosario Pontificia Universidad Católica Argentina (UCA)Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Departamento de Biologia Geral e Aplicada Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Universidade Estadual da Paraíba (UEPB)Universidade de São Paulo (USP)Universidade Federal do Pará (UFPA)Universidade Federal de Viçosa (UFV)Universidad Privada Norbert WienerPontificia Universidad Católica Argentina (UCA)Universidade Estadual Paulista (UNESP)Machado, SuellenFeitosa, ValkerPillaca-Pullo, OmarLario, LucianaSette, Lara [UNESP]Pessoa, AdalbertoAlves, Harley2023-07-29T12:54:53Z2023-07-29T12:54:53Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/bioengineering9110694Bioengineering, v. 9, n. 11, 2022.2306-5354http://hdl.handle.net/11449/24694810.3390/bioengineering91106942-s2.0-85149473921Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioengineeringinfo:eu-repo/semantics/openAccess2023-07-29T12:54:53Zoai:repositorio.unesp.br:11449/246948Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:18:49.911514Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
spellingShingle |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor Machado, Suellen bioreactor extremophilic oxygen transference proteinase proteolytic psychrophilic |
title_short |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_full |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_fullStr |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_full_unstemmed |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
title_sort |
Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor |
author |
Machado, Suellen |
author_facet |
Machado, Suellen Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara [UNESP] Pessoa, Adalberto Alves, Harley |
author_role |
author |
author2 |
Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara [UNESP] Pessoa, Adalberto Alves, Harley |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual da Paraíba (UEPB) Universidade de São Paulo (USP) Universidade Federal do Pará (UFPA) Universidade Federal de Viçosa (UFV) Universidad Privada Norbert Wiener Pontificia Universidad Católica Argentina (UCA) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Machado, Suellen Feitosa, Valker Pillaca-Pullo, Omar Lario, Luciana Sette, Lara [UNESP] Pessoa, Adalberto Alves, Harley |
dc.subject.por.fl_str_mv |
bioreactor extremophilic oxygen transference proteinase proteolytic psychrophilic |
topic |
bioreactor extremophilic oxygen transference proteinase proteolytic psychrophilic |
description |
Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T12:54:53Z 2023-07-29T12:54:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/bioengineering9110694 Bioengineering, v. 9, n. 11, 2022. 2306-5354 http://hdl.handle.net/11449/246948 10.3390/bioengineering9110694 2-s2.0-85149473921 |
url |
http://dx.doi.org/10.3390/bioengineering9110694 http://hdl.handle.net/11449/246948 |
identifier_str_mv |
Bioengineering, v. 9, n. 11, 2022. 2306-5354 10.3390/bioengineering9110694 2-s2.0-85149473921 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioengineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128496186163200 |