Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Daniel Araki
Data de Publicação: 2009
Outros Autores: Fracalossi, Ana Carolina Cuzzuol, Uatari, Silvia Akemi, Oshima, Celina Tizuko Fujiyama, Salvadori, Daisy Maria Favero [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/225751
Resumo: This study was undertaken to investigate, by immunohistochemistry, the expression of some tumor suppressor genes such as p16, p21 and Retinoblastoma (Rb) during 4-Nitroquinoline 1-oxide induced rat tongue carcinogenesis. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Neither histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, nor statistically significant differences (p>0.05) in expression of all the tumor suppressor genes were found when compared to the negative control. However, the levels of Rb were increased (p<0.05) in pre-neoplastic lesions at 12 weeks following carcinogen exposure. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, p16 and Rb were expressed in some tumor cells. Taken together, the results support the belief that the expression of Rb is closely event-related to malignant transformation and conversion of the oral mucosa, being a reliable biomarker linked to oral cancer pathogenesis.
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spelling Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide4-nitroquinoline 1-oxideMalignant transformationOral squamous cell carcinomaP16p21Rat tongue mucosaRbThis study was undertaken to investigate, by immunohistochemistry, the expression of some tumor suppressor genes such as p16, p21 and Retinoblastoma (Rb) during 4-Nitroquinoline 1-oxide induced rat tongue carcinogenesis. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Neither histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, nor statistically significant differences (p>0.05) in expression of all the tumor suppressor genes were found when compared to the negative control. However, the levels of Rb were increased (p<0.05) in pre-neoplastic lesions at 12 weeks following carcinogen exposure. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, p16 and Rb were expressed in some tumor cells. Taken together, the results support the belief that the expression of Rb is closely event-related to malignant transformation and conversion of the oral mucosa, being a reliable biomarker linked to oral cancer pathogenesis.Department of Pathology Federal University of Sao Paulo UNIFESP, SPDepartment of Biosciences Federal University of Sao Paulo UNIFESP, SPDepartment of Pathology Botucatu Medical School Sao Paulo State University UNESP, SPDepartment of Pathology Botucatu Medical School Sao Paulo State University UNESP, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Ribeiro, Daniel ArakiFracalossi, Ana Carolina CuzzuolUatari, Silvia AkemiOshima, Celina Tizuko FujiyamaSalvadori, Daisy Maria Favero [UNESP]2022-04-28T20:59:30Z2022-04-28T20:59:30Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article937-942In Vivo, v. 23, n. 6, p. 937-942, 2009.0258-851Xhttp://hdl.handle.net/11449/2257512-s2.0-75149134735Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIn Vivoinfo:eu-repo/semantics/openAccess2024-09-03T13:14:42Zoai:repositorio.unesp.br:11449/225751Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
title Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
spellingShingle Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
Ribeiro, Daniel Araki
4-nitroquinoline 1-oxide
Malignant transformation
Oral squamous cell carcinoma
P16
p21
Rat tongue mucosa
Rb
title_short Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
title_full Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
title_fullStr Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
title_full_unstemmed Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
title_sort Imbalance of tumor suppression genes expression following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
author Ribeiro, Daniel Araki
author_facet Ribeiro, Daniel Araki
Fracalossi, Ana Carolina Cuzzuol
Uatari, Silvia Akemi
Oshima, Celina Tizuko Fujiyama
Salvadori, Daisy Maria Favero [UNESP]
author_role author
author2 Fracalossi, Ana Carolina Cuzzuol
Uatari, Silvia Akemi
Oshima, Celina Tizuko Fujiyama
Salvadori, Daisy Maria Favero [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Ribeiro, Daniel Araki
Fracalossi, Ana Carolina Cuzzuol
Uatari, Silvia Akemi
Oshima, Celina Tizuko Fujiyama
Salvadori, Daisy Maria Favero [UNESP]
dc.subject.por.fl_str_mv 4-nitroquinoline 1-oxide
Malignant transformation
Oral squamous cell carcinoma
P16
p21
Rat tongue mucosa
Rb
topic 4-nitroquinoline 1-oxide
Malignant transformation
Oral squamous cell carcinoma
P16
p21
Rat tongue mucosa
Rb
description This study was undertaken to investigate, by immunohistochemistry, the expression of some tumor suppressor genes such as p16, p21 and Retinoblastoma (Rb) during 4-Nitroquinoline 1-oxide induced rat tongue carcinogenesis. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Neither histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, nor statistically significant differences (p>0.05) in expression of all the tumor suppressor genes were found when compared to the negative control. However, the levels of Rb were increased (p<0.05) in pre-neoplastic lesions at 12 weeks following carcinogen exposure. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, p16 and Rb were expressed in some tumor cells. Taken together, the results support the belief that the expression of Rb is closely event-related to malignant transformation and conversion of the oral mucosa, being a reliable biomarker linked to oral cancer pathogenesis.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2022-04-28T20:59:30Z
2022-04-28T20:59:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv In Vivo, v. 23, n. 6, p. 937-942, 2009.
0258-851X
http://hdl.handle.net/11449/225751
2-s2.0-75149134735
identifier_str_mv In Vivo, v. 23, n. 6, p. 937-942, 2009.
0258-851X
2-s2.0-75149134735
url http://hdl.handle.net/11449/225751
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv In Vivo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 937-942
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021372786638848

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