Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis

Detalhes bibliográficos
Autor(a) principal: Maltempe, Flaviane Granero
Data de Publicação: 2017
Outros Autores: Caleffi-Ferracioli, Katiany Rizzieri, Ribeiro do Amaral, Renata Claro, Demitto, Fernanda de Oliveira, Dias Siqueira, Vera Lucia, Lima Scodro, Regiane Bertin de, Hirata, Mario Hiroyuki, Pavan, Fernando Rogerio [UNESP], Cardoso, Rosilene Fressatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.tube.2017.02.004
http://hdl.handle.net/11449/162785
Resumo: Background: Linezolid (LZD) is not commonly used for treating tuberculosis (TB), but in some patients with drug-resistant TB it is being used. However, the in vitro LZD activity, in combination with rifampicin (RIF) against Mycobacterium tuberculosis has not been fully elucidated. Aims: The aim of this study was to evaluate the in vitro activity of RIF/LZD combination against M. tuberculosis clinical isolates. Materials and methods: The activity of the RIF/LZD combination was firstly determined in M. tuberculosis H(37)Rv, 14 susceptible, 9 isoniazid nonresistant and 14 multi-drug resistant (MDR) M. tuberculosis clinical isolates by modified checkerboard assay, Resazurin Drugs Combination Microtiter Assay (REDCA). After, the Time Kill Curve Assay, at 0.5 x MIC of drugs, in combination and alone, was performed in M. tuberculosis H(37)Rv and 8 (20.5%) of those clinical isolates, which the RIF/LZD combination showed to have synergistic effect by the checkerboard assay. Results and conclusion: By Time Kill Curve Assay, we could observe in M. tuberculosis H(37)Rv and susceptible isolates, that LZD alone, at sub inhibitory concentration, has poor effect on the bacillus death. In some cases, the bacillus growth stayed constant while in others showed regrowth at the eighth day of drug exposure. RIF alone exhibits potent concentration-dependent bactericidal activity, and was strongly dependent by the drug exposure time. The RIF/LZD combination accomplished a bacteriostatic effect in the reference strain and susceptible isolates. For the RIF resistant isolates, the RIF/LZD combination did not enhance the effect in killing bacillus. In this sense, additional, in vitro and in vivo studies are needed to evaluate the effect of RIF/LZD combination in order to better understand the adjunctive action of LZD in the treatment of TB and prevent the emergence of mutants with resistance to the available anti-TB drugs. (C) 2017 Elsevier Ltd. All rights reserved.
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spelling Activity of rifampicin and linezolid combination in Mycobacterium tuberculosisDrug resistanceTreatmentTuberculosisLinezolid and rifampicin combinationBackground: Linezolid (LZD) is not commonly used for treating tuberculosis (TB), but in some patients with drug-resistant TB it is being used. However, the in vitro LZD activity, in combination with rifampicin (RIF) against Mycobacterium tuberculosis has not been fully elucidated. Aims: The aim of this study was to evaluate the in vitro activity of RIF/LZD combination against M. tuberculosis clinical isolates. Materials and methods: The activity of the RIF/LZD combination was firstly determined in M. tuberculosis H(37)Rv, 14 susceptible, 9 isoniazid nonresistant and 14 multi-drug resistant (MDR) M. tuberculosis clinical isolates by modified checkerboard assay, Resazurin Drugs Combination Microtiter Assay (REDCA). After, the Time Kill Curve Assay, at 0.5 x MIC of drugs, in combination and alone, was performed in M. tuberculosis H(37)Rv and 8 (20.5%) of those clinical isolates, which the RIF/LZD combination showed to have synergistic effect by the checkerboard assay. Results and conclusion: By Time Kill Curve Assay, we could observe in M. tuberculosis H(37)Rv and susceptible isolates, that LZD alone, at sub inhibitory concentration, has poor effect on the bacillus death. In some cases, the bacillus growth stayed constant while in others showed regrowth at the eighth day of drug exposure. RIF alone exhibits potent concentration-dependent bactericidal activity, and was strongly dependent by the drug exposure time. The RIF/LZD combination accomplished a bacteriostatic effect in the reference strain and susceptible isolates. For the RIF resistant isolates, the RIF/LZD combination did not enhance the effect in killing bacillus. In this sense, additional, in vitro and in vivo studies are needed to evaluate the effect of RIF/LZD combination in order to better understand the adjunctive action of LZD in the treatment of TB and prevent the emergence of mutants with resistance to the available anti-TB drugs. (C) 2017 Elsevier Ltd. All rights reserved.Univ Estadual Maringa, Postgrad Biosci & Physiopathol, Ave Colombo 5790, BR-87020900 Maringa, Parana, BrazilUniv Estadual Maringa, Dept Clin Anal & Biomed, Lab Teaching & Res Clin Anal LEPAC, Lab Med Bacteriol, Ave Colombo 5790, BR-87020900 Maringa, Parana, BrazilUniv Estadual Maringa, Postgrad Hlth Sci, Ave Colombo 5790, BR-87020900 Maringa, Parana, BrazilUniv Sao Paulo, Sch Pharmaceut Sci, BR-05508900 Sao Paulo, BrazilUniv Estadual Paulista, Dept Biol Sci, Sch Pharmaceut Sci, BR-14800901 Sao Paulo, BrazilUniv Estadual Paulista, Dept Biol Sci, Sch Pharmaceut Sci, BR-14800901 Sao Paulo, BrazilChurchill LivingstoneUniversidade Estadual de Maringá (UEM)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Maltempe, Flaviane GraneroCaleffi-Ferracioli, Katiany RizzieriRibeiro do Amaral, Renata ClaroDemitto, Fernanda de OliveiraDias Siqueira, Vera LuciaLima Scodro, Regiane Bertin deHirata, Mario HiroyukiPavan, Fernando Rogerio [UNESP]Cardoso, Rosilene Fressatti2018-11-26T17:31:23Z2018-11-26T17:31:23Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24-29application/pdfhttp://dx.doi.org/10.1016/j.tube.2017.02.004Tuberculosis. Edinburgh: Churchill Livingstone, v. 104, p. 24-29, 2017.1472-9792http://hdl.handle.net/11449/16278510.1016/j.tube.2017.02.004WOS:000401127100004WOS000401127100004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTuberculosis1,264info:eu-repo/semantics/openAccess2024-06-24T13:07:37Zoai:repositorio.unesp.br:11449/162785Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:26:29.111457Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
title Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
spellingShingle Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
Maltempe, Flaviane Granero
Drug resistance
Treatment
Tuberculosis
Linezolid and rifampicin combination
title_short Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
title_full Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
title_fullStr Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
title_full_unstemmed Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
title_sort Activity of rifampicin and linezolid combination in Mycobacterium tuberculosis
author Maltempe, Flaviane Granero
author_facet Maltempe, Flaviane Granero
Caleffi-Ferracioli, Katiany Rizzieri
Ribeiro do Amaral, Renata Claro
Demitto, Fernanda de Oliveira
Dias Siqueira, Vera Lucia
Lima Scodro, Regiane Bertin de
Hirata, Mario Hiroyuki
Pavan, Fernando Rogerio [UNESP]
Cardoso, Rosilene Fressatti
author_role author
author2 Caleffi-Ferracioli, Katiany Rizzieri
Ribeiro do Amaral, Renata Claro
Demitto, Fernanda de Oliveira
Dias Siqueira, Vera Lucia
Lima Scodro, Regiane Bertin de
Hirata, Mario Hiroyuki
Pavan, Fernando Rogerio [UNESP]
Cardoso, Rosilene Fressatti
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Maltempe, Flaviane Granero
Caleffi-Ferracioli, Katiany Rizzieri
Ribeiro do Amaral, Renata Claro
Demitto, Fernanda de Oliveira
Dias Siqueira, Vera Lucia
Lima Scodro, Regiane Bertin de
Hirata, Mario Hiroyuki
Pavan, Fernando Rogerio [UNESP]
Cardoso, Rosilene Fressatti
dc.subject.por.fl_str_mv Drug resistance
Treatment
Tuberculosis
Linezolid and rifampicin combination
topic Drug resistance
Treatment
Tuberculosis
Linezolid and rifampicin combination
description Background: Linezolid (LZD) is not commonly used for treating tuberculosis (TB), but in some patients with drug-resistant TB it is being used. However, the in vitro LZD activity, in combination with rifampicin (RIF) against Mycobacterium tuberculosis has not been fully elucidated. Aims: The aim of this study was to evaluate the in vitro activity of RIF/LZD combination against M. tuberculosis clinical isolates. Materials and methods: The activity of the RIF/LZD combination was firstly determined in M. tuberculosis H(37)Rv, 14 susceptible, 9 isoniazid nonresistant and 14 multi-drug resistant (MDR) M. tuberculosis clinical isolates by modified checkerboard assay, Resazurin Drugs Combination Microtiter Assay (REDCA). After, the Time Kill Curve Assay, at 0.5 x MIC of drugs, in combination and alone, was performed in M. tuberculosis H(37)Rv and 8 (20.5%) of those clinical isolates, which the RIF/LZD combination showed to have synergistic effect by the checkerboard assay. Results and conclusion: By Time Kill Curve Assay, we could observe in M. tuberculosis H(37)Rv and susceptible isolates, that LZD alone, at sub inhibitory concentration, has poor effect on the bacillus death. In some cases, the bacillus growth stayed constant while in others showed regrowth at the eighth day of drug exposure. RIF alone exhibits potent concentration-dependent bactericidal activity, and was strongly dependent by the drug exposure time. The RIF/LZD combination accomplished a bacteriostatic effect in the reference strain and susceptible isolates. For the RIF resistant isolates, the RIF/LZD combination did not enhance the effect in killing bacillus. In this sense, additional, in vitro and in vivo studies are needed to evaluate the effect of RIF/LZD combination in order to better understand the adjunctive action of LZD in the treatment of TB and prevent the emergence of mutants with resistance to the available anti-TB drugs. (C) 2017 Elsevier Ltd. All rights reserved.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
2018-11-26T17:31:23Z
2018-11-26T17:31:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.tube.2017.02.004
Tuberculosis. Edinburgh: Churchill Livingstone, v. 104, p. 24-29, 2017.
1472-9792
http://hdl.handle.net/11449/162785
10.1016/j.tube.2017.02.004
WOS:000401127100004
WOS000401127100004.pdf
url http://dx.doi.org/10.1016/j.tube.2017.02.004
http://hdl.handle.net/11449/162785
identifier_str_mv Tuberculosis. Edinburgh: Churchill Livingstone, v. 104, p. 24-29, 2017.
1472-9792
10.1016/j.tube.2017.02.004
WOS:000401127100004
WOS000401127100004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tuberculosis
1,264
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 24-29
application/pdf
dc.publisher.none.fl_str_mv Churchill Livingstone
publisher.none.fl_str_mv Churchill Livingstone
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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