Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jmmm.2020.167326 http://hdl.handle.net/11449/202066 |
Resumo: | A magnetic cross-linked enzyme aggregate (MCLEA) of cellulases and a non-magnetic analogue (CLEA) were synthesized and further investigated. The obtained MCLEA and CLEA showed a high enzyme loading efficiency of 90% and 88%, respectively. However, the MCLEA presented an activity 33% higher than the CLEA one, suggesting a catalytic enhancement effect as a result of magnetic nanoparticle presence in cross-linked cellulase aggregates structure. In addition, MCLEA shows an unusual behavior with highest enzymatic activity at low temperatures near to 37 °C. Lastly, a full factorial design (2x2) with two variables at two levels, frequency (203–420 kHz) and amplitude (3–6 kA m−1), was used in order to better investigate the effects of an alternating magnetic field (AMF) on MCLEA activity. AMF application decreased MCLEA activity, particularly in the highest values of frequency and amplitude, when compared to condition field free. In addition, frequency implied in more significant effects on MCLEA activity when compared to AMF amplitude. |
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Repositório Institucional da UNESP |
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Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activityAlternating magnetic fieldEnzyme immobilizationMagnetic nanoparticlesMagnetic supportsA magnetic cross-linked enzyme aggregate (MCLEA) of cellulases and a non-magnetic analogue (CLEA) were synthesized and further investigated. The obtained MCLEA and CLEA showed a high enzyme loading efficiency of 90% and 88%, respectively. However, the MCLEA presented an activity 33% higher than the CLEA one, suggesting a catalytic enhancement effect as a result of magnetic nanoparticle presence in cross-linked cellulase aggregates structure. In addition, MCLEA shows an unusual behavior with highest enzymatic activity at low temperatures near to 37 °C. Lastly, a full factorial design (2x2) with two variables at two levels, frequency (203–420 kHz) and amplitude (3–6 kA m−1), was used in order to better investigate the effects of an alternating magnetic field (AMF) on MCLEA activity. AMF application decreased MCLEA activity, particularly in the highest values of frequency and amplitude, when compared to condition field free. In addition, frequency implied in more significant effects on MCLEA activity when compared to AMF amplitude.São Paulo State University (UNESP) Institute of Chemistry Magnetic Materials and Colloids LaboratorySão Paulo State University (UNESP) Institute of Chemistry Institute for Bioenergy Research (IPBEN)São Paulo State University (UNESP) School of Pharmaceutical SciencesSão Paulo State University (UNESP) Institute of Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives (CEMPEQC)São Paulo State University (UNESP) Institute of Chemistry Magnetic Materials and Colloids LaboratorySão Paulo State University (UNESP) Institute of Chemistry Institute for Bioenergy Research (IPBEN)São Paulo State University (UNESP) School of Pharmaceutical SciencesSão Paulo State University (UNESP) Institute of Chemistry Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives (CEMPEQC)Universidade Estadual Paulista (Unesp)Lucena, Guilherme N. [UNESP]Santos, Caio C. dos [UNESP]Pinto, Gabriel C. [UNESP]Piazza, Rodolfo D. [UNESP]Guedes, Wesley N. [UNESP]Jafelicci Júnior, Miguel [UNESP]de Paula, Ariela V. [UNESP]Marques, Rodrigo Fernando C. [UNESP]2020-12-12T02:48:57Z2020-12-12T02:48:57Z2020-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jmmm.2020.167326Journal of Magnetism and Magnetic Materials, v. 516.0304-8853http://hdl.handle.net/11449/20206610.1016/j.jmmm.2020.1673262-s2.0-850902236517182671143702858Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Magnetism and Magnetic Materialsinfo:eu-repo/semantics/openAccess2021-10-23T10:18:36Zoai:repositorio.unesp.br:11449/202066Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:30:38.404241Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
title |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
spellingShingle |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity Lucena, Guilherme N. [UNESP] Alternating magnetic field Enzyme immobilization Magnetic nanoparticles Magnetic supports |
title_short |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
title_full |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
title_fullStr |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
title_full_unstemmed |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
title_sort |
Synthesis and characterization of magnetic cross-linked enzyme aggregate and its evaluation of the alternating magnetic field (AMF) effects in the catalytic activity |
author |
Lucena, Guilherme N. [UNESP] |
author_facet |
Lucena, Guilherme N. [UNESP] Santos, Caio C. dos [UNESP] Pinto, Gabriel C. [UNESP] Piazza, Rodolfo D. [UNESP] Guedes, Wesley N. [UNESP] Jafelicci Júnior, Miguel [UNESP] de Paula, Ariela V. [UNESP] Marques, Rodrigo Fernando C. [UNESP] |
author_role |
author |
author2 |
Santos, Caio C. dos [UNESP] Pinto, Gabriel C. [UNESP] Piazza, Rodolfo D. [UNESP] Guedes, Wesley N. [UNESP] Jafelicci Júnior, Miguel [UNESP] de Paula, Ariela V. [UNESP] Marques, Rodrigo Fernando C. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lucena, Guilherme N. [UNESP] Santos, Caio C. dos [UNESP] Pinto, Gabriel C. [UNESP] Piazza, Rodolfo D. [UNESP] Guedes, Wesley N. [UNESP] Jafelicci Júnior, Miguel [UNESP] de Paula, Ariela V. [UNESP] Marques, Rodrigo Fernando C. [UNESP] |
dc.subject.por.fl_str_mv |
Alternating magnetic field Enzyme immobilization Magnetic nanoparticles Magnetic supports |
topic |
Alternating magnetic field Enzyme immobilization Magnetic nanoparticles Magnetic supports |
description |
A magnetic cross-linked enzyme aggregate (MCLEA) of cellulases and a non-magnetic analogue (CLEA) were synthesized and further investigated. The obtained MCLEA and CLEA showed a high enzyme loading efficiency of 90% and 88%, respectively. However, the MCLEA presented an activity 33% higher than the CLEA one, suggesting a catalytic enhancement effect as a result of magnetic nanoparticle presence in cross-linked cellulase aggregates structure. In addition, MCLEA shows an unusual behavior with highest enzymatic activity at low temperatures near to 37 °C. Lastly, a full factorial design (2x2) with two variables at two levels, frequency (203–420 kHz) and amplitude (3–6 kA m−1), was used in order to better investigate the effects of an alternating magnetic field (AMF) on MCLEA activity. AMF application decreased MCLEA activity, particularly in the highest values of frequency and amplitude, when compared to condition field free. In addition, frequency implied in more significant effects on MCLEA activity when compared to AMF amplitude. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:48:57Z 2020-12-12T02:48:57Z 2020-12-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jmmm.2020.167326 Journal of Magnetism and Magnetic Materials, v. 516. 0304-8853 http://hdl.handle.net/11449/202066 10.1016/j.jmmm.2020.167326 2-s2.0-85090223651 7182671143702858 |
url |
http://dx.doi.org/10.1016/j.jmmm.2020.167326 http://hdl.handle.net/11449/202066 |
identifier_str_mv |
Journal of Magnetism and Magnetic Materials, v. 516. 0304-8853 10.1016/j.jmmm.2020.167326 2-s2.0-85090223651 7182671143702858 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Magnetism and Magnetic Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128818849775616 |