Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/03601234.2020.1766898 http://hdl.handle.net/11449/201806 |
Resumo: | The objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent® WS 800 and Curbix® SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of Oreochromis niloticus. The analyses of HSP70 shock protein labelling and cell death process by TUNEL method were performed in order to measure the effects of the exposure of cell repair system of fish to both insecticides. Statistical analyses showed no significant molecular damage to the hepatic tissue of animals. Nevertheless, variations in HSP70 and DNA fragmentation levels, endpoint of cell repair system response and cellular death, respectively, were observed in several groups. These results indicate that the cell repair machinery was efficient when in contact with residual concentrations of insecticides. However, the DNA fragmentation detected by the TUNEL method suggests that even in face of the cytoprotective action of the HSP70 protein, there are damages that become irreparable. To finish, it is worth mentioning that given the results obtained from residual concentrations, use in the field should be with caution. |
id |
UNSP_65b3c845f79a2b063453157a4695bd16 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/201806 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiproleimmunostainingphenylpyrazoleStress proteinTilápiaTUNELThe objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent® WS 800 and Curbix® SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of Oreochromis niloticus. The analyses of HSP70 shock protein labelling and cell death process by TUNEL method were performed in order to measure the effects of the exposure of cell repair system of fish to both insecticides. Statistical analyses showed no significant molecular damage to the hepatic tissue of animals. Nevertheless, variations in HSP70 and DNA fragmentation levels, endpoint of cell repair system response and cellular death, respectively, were observed in several groups. These results indicate that the cell repair machinery was efficient when in contact with residual concentrations of insecticides. However, the DNA fragmentation detected by the TUNEL method suggests that even in face of the cytoprotective action of the HSP70 protein, there are damages that become irreparable. To finish, it is worth mentioning that given the results obtained from residual concentrations, use in the field should be with caution.Department of Biology UNESP (São Paulo State University) IB (Instituto de Biociências)Department of Biology UNESP (São Paulo State University) IB (Instituto de Biociências)Universidade Estadual Paulista (Unesp)Guedes, Thays de Andrade [UNESP]Moreira-de-Sousa, Cristina [UNESP]Lima, Hellen Maria Soares [UNESP]Grella, Tatiane Caroline [UNESP]Socolowski, Priscila Cintra [UNESP]Fontanetti, Carmem Silvia [UNESP]2020-12-12T02:42:19Z2020-12-12T02:42:19Z2020-08-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article687-693http://dx.doi.org/10.1080/03601234.2020.1766898Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, v. 55, n. 8, p. 687-693, 2020.1532-41090360-1234http://hdl.handle.net/11449/20180610.1080/03601234.2020.17668982-s2.0-85085366548Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastesinfo:eu-repo/semantics/openAccess2021-10-22T22:17:30Zoai:repositorio.unesp.br:11449/201806Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:44:03.601444Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
title |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
spellingShingle |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole Guedes, Thays de Andrade [UNESP] immunostaining phenylpyrazole Stress protein Tilápia TUNEL |
title_short |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
title_full |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
title_fullStr |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
title_full_unstemmed |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
title_sort |
Cytoprotective and anti-apoptotic action of HSP70 stress protein in Oreochromis niloticus exposed to residual dilutions of insecticides with fipronil and ethiprole |
author |
Guedes, Thays de Andrade [UNESP] |
author_facet |
Guedes, Thays de Andrade [UNESP] Moreira-de-Sousa, Cristina [UNESP] Lima, Hellen Maria Soares [UNESP] Grella, Tatiane Caroline [UNESP] Socolowski, Priscila Cintra [UNESP] Fontanetti, Carmem Silvia [UNESP] |
author_role |
author |
author2 |
Moreira-de-Sousa, Cristina [UNESP] Lima, Hellen Maria Soares [UNESP] Grella, Tatiane Caroline [UNESP] Socolowski, Priscila Cintra [UNESP] Fontanetti, Carmem Silvia [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Guedes, Thays de Andrade [UNESP] Moreira-de-Sousa, Cristina [UNESP] Lima, Hellen Maria Soares [UNESP] Grella, Tatiane Caroline [UNESP] Socolowski, Priscila Cintra [UNESP] Fontanetti, Carmem Silvia [UNESP] |
dc.subject.por.fl_str_mv |
immunostaining phenylpyrazole Stress protein Tilápia TUNEL |
topic |
immunostaining phenylpyrazole Stress protein Tilápia TUNEL |
description |
The objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent® WS 800 and Curbix® SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of Oreochromis niloticus. The analyses of HSP70 shock protein labelling and cell death process by TUNEL method were performed in order to measure the effects of the exposure of cell repair system of fish to both insecticides. Statistical analyses showed no significant molecular damage to the hepatic tissue of animals. Nevertheless, variations in HSP70 and DNA fragmentation levels, endpoint of cell repair system response and cellular death, respectively, were observed in several groups. These results indicate that the cell repair machinery was efficient when in contact with residual concentrations of insecticides. However, the DNA fragmentation detected by the TUNEL method suggests that even in face of the cytoprotective action of the HSP70 protein, there are damages that become irreparable. To finish, it is worth mentioning that given the results obtained from residual concentrations, use in the field should be with caution. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:42:19Z 2020-12-12T02:42:19Z 2020-08-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/03601234.2020.1766898 Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, v. 55, n. 8, p. 687-693, 2020. 1532-4109 0360-1234 http://hdl.handle.net/11449/201806 10.1080/03601234.2020.1766898 2-s2.0-85085366548 |
url |
http://dx.doi.org/10.1080/03601234.2020.1766898 http://hdl.handle.net/11449/201806 |
identifier_str_mv |
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, v. 55, n. 8, p. 687-693, 2020. 1532-4109 0360-1234 10.1080/03601234.2020.1766898 2-s2.0-85085366548 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
687-693 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128972289998848 |