Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-019-07203-6 http://hdl.handle.net/11449/201414 |
Resumo: | Millipedes are organisms of the edaphic fauna and have been used as bioindicators for the evaluation of pollutants in the environment, as they are in constant contact with the soil. This study used the millipede Rhinocricus padbergi as surrogate to evaluate the toxicity of two metallic-insecticides that has been developed for leaf-cutting ants management. Millipedes were exposed in terrariums containing different concentrations of the metallic-insecticides and, after periods of 21 and 90 days, three individuals from each terrarium were dissected in order to remove the midgut, the organ where absorption of nutrients and, consequently, toxic substances occurs. The toxic action of the metallic-insecticides was analyzed through qualitative and semi-quantitative analysis of morphophysiological alterations and by quantitative analysis of the HSP70 stress protein. The results showed that the metallic-insecticides may increase HSP70 labeling, although not at all concentrations and periods of exposure. Histopathological alterations were not significant at any concentration, indicating that the cytoprotective action of HSP70 is able to prevent severe damage to the midgut. It is therefore suggested that the metallic-insecticides are not toxic to the species studied here as no toxicity was observed under the conditions tested. In addition, stress protein localization in midgut helps understand how morphophysiological processes can potentially be affected by pesticide exposure. |
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Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticidesDiplopodFormicideHeat shock proteinHistochemistryHistologyImmunohistochemistryMillipedes are organisms of the edaphic fauna and have been used as bioindicators for the evaluation of pollutants in the environment, as they are in constant contact with the soil. This study used the millipede Rhinocricus padbergi as surrogate to evaluate the toxicity of two metallic-insecticides that has been developed for leaf-cutting ants management. Millipedes were exposed in terrariums containing different concentrations of the metallic-insecticides and, after periods of 21 and 90 days, three individuals from each terrarium were dissected in order to remove the midgut, the organ where absorption of nutrients and, consequently, toxic substances occurs. The toxic action of the metallic-insecticides was analyzed through qualitative and semi-quantitative analysis of morphophysiological alterations and by quantitative analysis of the HSP70 stress protein. The results showed that the metallic-insecticides may increase HSP70 labeling, although not at all concentrations and periods of exposure. Histopathological alterations were not significant at any concentration, indicating that the cytoprotective action of HSP70 is able to prevent severe damage to the midgut. It is therefore suggested that the metallic-insecticides are not toxic to the species studied here as no toxicity was observed under the conditions tested. In addition, stress protein localization in midgut helps understand how morphophysiological processes can potentially be affected by pesticide exposure.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Bioscience UNESP - São Paulo State UniversitySchool of Civil Engineering Architecture and Urban Design University of CampinasInstitute of Bioscience UNESP - São Paulo State UniversityFAPESP: 2012/12019-5Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)de Souza, Raphael B. [UNESP]Moreira-de-Sousa, Cristina [UNESP]Ansoar-Rodríguez, Yadira [UNESP]Coelho, Maria Paula Mancini [UNESP]de Souza, Cleiton Pereira [UNESP]Bueno, Odair Correa [UNESP]Fontanetti, Carmem S. [UNESP]2020-12-12T02:31:56Z2020-12-12T02:31:56Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3023-3033http://dx.doi.org/10.1007/s11356-019-07203-6Environmental Science and Pollution Research, v. 27, n. 3, p. 3023-3033, 2020.1614-74990944-1344http://hdl.handle.net/11449/20141410.1007/s11356-019-07203-62-s2.0-850768410601050709055776428Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2021-10-22T19:38:59Zoai:repositorio.unesp.br:11449/201414Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:01.724786Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
title |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
spellingShingle |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides de Souza, Raphael B. [UNESP] Diplopod Formicide Heat shock protein Histochemistry Histology Immunohistochemistry |
title_short |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
title_full |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
title_fullStr |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
title_full_unstemmed |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
title_sort |
Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides |
author |
de Souza, Raphael B. [UNESP] |
author_facet |
de Souza, Raphael B. [UNESP] Moreira-de-Sousa, Cristina [UNESP] Ansoar-Rodríguez, Yadira [UNESP] Coelho, Maria Paula Mancini [UNESP] de Souza, Cleiton Pereira [UNESP] Bueno, Odair Correa [UNESP] Fontanetti, Carmem S. [UNESP] |
author_role |
author |
author2 |
Moreira-de-Sousa, Cristina [UNESP] Ansoar-Rodríguez, Yadira [UNESP] Coelho, Maria Paula Mancini [UNESP] de Souza, Cleiton Pereira [UNESP] Bueno, Odair Correa [UNESP] Fontanetti, Carmem S. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
de Souza, Raphael B. [UNESP] Moreira-de-Sousa, Cristina [UNESP] Ansoar-Rodríguez, Yadira [UNESP] Coelho, Maria Paula Mancini [UNESP] de Souza, Cleiton Pereira [UNESP] Bueno, Odair Correa [UNESP] Fontanetti, Carmem S. [UNESP] |
dc.subject.por.fl_str_mv |
Diplopod Formicide Heat shock protein Histochemistry Histology Immunohistochemistry |
topic |
Diplopod Formicide Heat shock protein Histochemistry Histology Immunohistochemistry |
description |
Millipedes are organisms of the edaphic fauna and have been used as bioindicators for the evaluation of pollutants in the environment, as they are in constant contact with the soil. This study used the millipede Rhinocricus padbergi as surrogate to evaluate the toxicity of two metallic-insecticides that has been developed for leaf-cutting ants management. Millipedes were exposed in terrariums containing different concentrations of the metallic-insecticides and, after periods of 21 and 90 days, three individuals from each terrarium were dissected in order to remove the midgut, the organ where absorption of nutrients and, consequently, toxic substances occurs. The toxic action of the metallic-insecticides was analyzed through qualitative and semi-quantitative analysis of morphophysiological alterations and by quantitative analysis of the HSP70 stress protein. The results showed that the metallic-insecticides may increase HSP70 labeling, although not at all concentrations and periods of exposure. Histopathological alterations were not significant at any concentration, indicating that the cytoprotective action of HSP70 is able to prevent severe damage to the midgut. It is therefore suggested that the metallic-insecticides are not toxic to the species studied here as no toxicity was observed under the conditions tested. In addition, stress protein localization in midgut helps understand how morphophysiological processes can potentially be affected by pesticide exposure. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:31:56Z 2020-12-12T02:31:56Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-019-07203-6 Environmental Science and Pollution Research, v. 27, n. 3, p. 3023-3033, 2020. 1614-7499 0944-1344 http://hdl.handle.net/11449/201414 10.1007/s11356-019-07203-6 2-s2.0-85076841060 1050709055776428 |
url |
http://dx.doi.org/10.1007/s11356-019-07203-6 http://hdl.handle.net/11449/201414 |
identifier_str_mv |
Environmental Science and Pollution Research, v. 27, n. 3, p. 3023-3033, 2020. 1614-7499 0944-1344 10.1007/s11356-019-07203-6 2-s2.0-85076841060 1050709055776428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3023-3033 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129573422891008 |