Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2012.03.030 http://hdl.handle.net/11449/14703 |
Resumo: | The objective was to evaluate ovarian activity reversibility in domestic queens after short-term contraceptive treatment with deslorelin acetate. Ten mature queens were used. In all queens, the estrous cycle was evaluated every 72 h by vaginal cytology (VC) and behavior assessments. When queens had VC characteristic of interestrus or diestrus, one deslorelin acetate implant (4.7 mg) was placed in the subcutaneous tissue of the interscapular region (day of insertion = Day 0). Thereafter, VC was performed every 48 h and on Day 90, implants were removed. At Day 100, estrus and ovulation were induced with 100 IU eCG (im), followed by 100 IU hCG (im), 84 h later (Day 103.5). Queens were ovariohysterectomized on Day 106. Corpora lutea (CL) were counted, oviducts were flushed, and oocytes were identified, isolated and stained to assess viability. In all queens, blood samples for plasma progesterone concentrations were collected once a week, from Days -21 to 106. After deslorelin acetate application, four queens had VC and behavior typical of estrus, and one ovulated. Furthermore, ovulation occurred in three queens that did not have VC or behavior consistent with estrus. After the initial ovarian stimulation, all females had anestrous VC during the deslorelin treatment period. Implants were readily removed. Following implant removal, all females responded to treatments to induce estrus and ovulation. There were (mean +/- SEM) 13.1 +/- 5.5 CL and 8.1 +/- 5.5 oocytes per queen; the oocyte recovery rate was 56.8 +/- 25.4% and all recovered oocytes were viable. We concluded that deslorelin acetate can be used as a reversible short-term contraceptive in domestic cats, because estrus and ovulation were successfully induced following implant removal. (C) 2012 Elsevier B.V. All rights reserved. |
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Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queensDeslorelin acetateContraceptionDomestic catOocyte recoveryThe objective was to evaluate ovarian activity reversibility in domestic queens after short-term contraceptive treatment with deslorelin acetate. Ten mature queens were used. In all queens, the estrous cycle was evaluated every 72 h by vaginal cytology (VC) and behavior assessments. When queens had VC characteristic of interestrus or diestrus, one deslorelin acetate implant (4.7 mg) was placed in the subcutaneous tissue of the interscapular region (day of insertion = Day 0). Thereafter, VC was performed every 48 h and on Day 90, implants were removed. At Day 100, estrus and ovulation were induced with 100 IU eCG (im), followed by 100 IU hCG (im), 84 h later (Day 103.5). Queens were ovariohysterectomized on Day 106. Corpora lutea (CL) were counted, oviducts were flushed, and oocytes were identified, isolated and stained to assess viability. In all queens, blood samples for plasma progesterone concentrations were collected once a week, from Days -21 to 106. After deslorelin acetate application, four queens had VC and behavior typical of estrus, and one ovulated. Furthermore, ovulation occurred in three queens that did not have VC or behavior consistent with estrus. After the initial ovarian stimulation, all females had anestrous VC during the deslorelin treatment period. Implants were readily removed. Following implant removal, all females responded to treatments to induce estrus and ovulation. There were (mean +/- SEM) 13.1 +/- 5.5 CL and 8.1 +/- 5.5 oocytes per queen; the oocyte recovery rate was 56.8 +/- 25.4% and all recovered oocytes were viable. We concluded that deslorelin acetate can be used as a reversible short-term contraceptive in domestic cats, because estrus and ovulation were successfully induced following implant removal. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista FMVZ, Fac Vet Med, Dept Anim Reprod & Vet Radiol, UNESP, Botucatu, SP, BrazilUniv Estadual Paulista FMVZ, Fac Vet Med, Dept Vet Surg & Anesthesiol, UNESP, Botucatu, SP, BrazilUniv Estadual Paulista FMVZ, Fac Vet Med, Dept Anim Reprod & Vet Radiol, UNESP, Botucatu, SP, BrazilUniv Estadual Paulista FMVZ, Fac Vet Med, Dept Vet Surg & Anesthesiol, UNESP, Botucatu, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Ackermann, C. L. [UNESP]Volpato, R. [UNESP]Destro, F. C. [UNESP]Trevisol, E. [UNESP]Ruas Sousa, N. [UNESP]Guaitolini, C. R. F. [UNESP]Derussi, A. A. P. [UNESP]Rascado, T. S. [UNESP]Lopes, Maria Denise [UNESP]2013-09-30T18:29:08Z2014-05-20T13:42:18Z2013-09-30T18:29:08Z2014-05-20T13:42:18Z2012-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article817-822application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2012.03.030Theriogenology. New York: Elsevier B.V., v. 78, n. 4, p. 817-822, 2012.0093-691Xhttp://hdl.handle.net/11449/1470310.1016/j.theriogenology.2012.03.030WOS:000307622200015WOS000307622200015.pdf6666129914663018Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenology2.136info:eu-repo/semantics/openAccess2024-09-09T14:01:40Zoai:repositorio.unesp.br:11449/14703Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
title |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
spellingShingle |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens Ackermann, C. L. [UNESP] Deslorelin acetate Contraception Domestic cat Oocyte recovery |
title_short |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
title_full |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
title_fullStr |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
title_full_unstemmed |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
title_sort |
Ovarian activity reversibility after the use of deslorelin acetate as a short-term contraceptive in domestic queens |
author |
Ackermann, C. L. [UNESP] |
author_facet |
Ackermann, C. L. [UNESP] Volpato, R. [UNESP] Destro, F. C. [UNESP] Trevisol, E. [UNESP] Ruas Sousa, N. [UNESP] Guaitolini, C. R. F. [UNESP] Derussi, A. A. P. [UNESP] Rascado, T. S. [UNESP] Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
Volpato, R. [UNESP] Destro, F. C. [UNESP] Trevisol, E. [UNESP] Ruas Sousa, N. [UNESP] Guaitolini, C. R. F. [UNESP] Derussi, A. A. P. [UNESP] Rascado, T. S. [UNESP] Lopes, Maria Denise [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ackermann, C. L. [UNESP] Volpato, R. [UNESP] Destro, F. C. [UNESP] Trevisol, E. [UNESP] Ruas Sousa, N. [UNESP] Guaitolini, C. R. F. [UNESP] Derussi, A. A. P. [UNESP] Rascado, T. S. [UNESP] Lopes, Maria Denise [UNESP] |
dc.subject.por.fl_str_mv |
Deslorelin acetate Contraception Domestic cat Oocyte recovery |
topic |
Deslorelin acetate Contraception Domestic cat Oocyte recovery |
description |
The objective was to evaluate ovarian activity reversibility in domestic queens after short-term contraceptive treatment with deslorelin acetate. Ten mature queens were used. In all queens, the estrous cycle was evaluated every 72 h by vaginal cytology (VC) and behavior assessments. When queens had VC characteristic of interestrus or diestrus, one deslorelin acetate implant (4.7 mg) was placed in the subcutaneous tissue of the interscapular region (day of insertion = Day 0). Thereafter, VC was performed every 48 h and on Day 90, implants were removed. At Day 100, estrus and ovulation were induced with 100 IU eCG (im), followed by 100 IU hCG (im), 84 h later (Day 103.5). Queens were ovariohysterectomized on Day 106. Corpora lutea (CL) were counted, oviducts were flushed, and oocytes were identified, isolated and stained to assess viability. In all queens, blood samples for plasma progesterone concentrations were collected once a week, from Days -21 to 106. After deslorelin acetate application, four queens had VC and behavior typical of estrus, and one ovulated. Furthermore, ovulation occurred in three queens that did not have VC or behavior consistent with estrus. After the initial ovarian stimulation, all females had anestrous VC during the deslorelin treatment period. Implants were readily removed. Following implant removal, all females responded to treatments to induce estrus and ovulation. There were (mean +/- SEM) 13.1 +/- 5.5 CL and 8.1 +/- 5.5 oocytes per queen; the oocyte recovery rate was 56.8 +/- 25.4% and all recovered oocytes were viable. We concluded that deslorelin acetate can be used as a reversible short-term contraceptive in domestic cats, because estrus and ovulation were successfully induced following implant removal. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 2013-09-30T18:29:08Z 2013-09-30T18:29:08Z 2014-05-20T13:42:18Z 2014-05-20T13:42:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2012.03.030 Theriogenology. New York: Elsevier B.V., v. 78, n. 4, p. 817-822, 2012. 0093-691X http://hdl.handle.net/11449/14703 10.1016/j.theriogenology.2012.03.030 WOS:000307622200015 WOS000307622200015.pdf 6666129914663018 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2012.03.030 http://hdl.handle.net/11449/14703 |
identifier_str_mv |
Theriogenology. New York: Elsevier B.V., v. 78, n. 4, p. 817-822, 2012. 0093-691X 10.1016/j.theriogenology.2012.03.030 WOS:000307622200015 WOS000307622200015.pdf 6666129914663018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology 2.136 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
817-822 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021334305996800 |