Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1678-9199-20-5 http://hdl.handle.net/11449/112346 |
Resumo: | Background: This study aimed to evaluate the occurrence of Leishmania spp. in dogs and cats from Botucatu, Sao Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50 cats, two (4%) were positive. In Campo Grande, 29 dog blood cultures (58%) were positive and all (100%) cats negative by this test. Polymerase chain reaction detected Leishmania spp. in 100% of dog and cat samples from Botucatu but found all the cats from Campo Grande to be negative. On the other hand, 36 dogs from Campo Grande were positive (72%) by the same technique. Immunofluorescent antibody test in Botucatu found 100% of dogs and cats non-reactive, while in Campo Grande, it detected positivity in 32 dogs (64%) and 15 cats (30%).Conclusions: The results show the importance of not only continuous epidemiological surveillance in areas not endemic for leishmaniasis, but also research for accurate diagnosis of this zoonosis. |
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Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reactionDogCatDiagnosisLeishmaniasisZoonosisBackground: This study aimed to evaluate the occurrence of Leishmania spp. in dogs and cats from Botucatu, Sao Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50 cats, two (4%) were positive. In Campo Grande, 29 dog blood cultures (58%) were positive and all (100%) cats negative by this test. Polymerase chain reaction detected Leishmania spp. in 100% of dog and cat samples from Botucatu but found all the cats from Campo Grande to be negative. On the other hand, 36 dogs from Campo Grande were positive (72%) by the same technique. Immunofluorescent antibody test in Botucatu found 100% of dogs and cats non-reactive, while in Campo Grande, it detected positivity in 32 dogs (64%) and 15 cats (30%).Conclusions: The results show the importance of not only continuous epidemiological surveillance in areas not endemic for leishmaniasis, but also research for accurate diagnosis of this zoonosis.Center for Zoonosis Control in Campo Grande, MS, BrazilFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Mineiro Inst Agr IMA, Uberaba, MG, BrazilUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Vet Hyg & Publ Hlth,Sch Vet Med & Anim Husb, Botucatu, SP, BrazilAgencia Paulista Tecnol Agronegocios APTA SAA, Polo Reg Ctr Oeste, BR-17030000 Bauru, SP, BrazilUniv Estadual Paulista, UNESP, Sao Paulo State Univ, Dept Vet Hyg & Publ Hlth,Sch Vet Med & Anim Husb, Botucatu, SP, BrazilBiomed Central Ltd.Mineiro Inst Agr IMAUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Campos Braga, Audrey RennoLangoni, Hélio [UNESP]Lucheis, Simone Baldini [UNESP]2014-12-03T13:10:38Z2014-12-03T13:10:38Z2014-02-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1186/1678-9199-20-5Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Biomed Central Ltd, v. 20, 7 p., 2014.1678-9199http://hdl.handle.net/11449/11234610.1186/1678-9199-20-5WOS:000334697100001WOS000334697100001.pdf5326072118518067Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-12-20T06:22:22Zoai:repositorio.unesp.br:11449/112346Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-20T06:22:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
title |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
spellingShingle |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction Campos Braga, Audrey Renno Dog Cat Diagnosis Leishmaniasis Zoonosis |
title_short |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
title_full |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
title_fullStr |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
title_full_unstemmed |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
title_sort |
Evaluation of canine and feline leishmaniasis by the association of blood culture, immunofluorescent antibody test and polymerase chain reaction |
author |
Campos Braga, Audrey Renno |
author_facet |
Campos Braga, Audrey Renno Langoni, Hélio [UNESP] Lucheis, Simone Baldini [UNESP] |
author_role |
author |
author2 |
Langoni, Hélio [UNESP] Lucheis, Simone Baldini [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Mineiro Inst Agr IMA Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Campos Braga, Audrey Renno Langoni, Hélio [UNESP] Lucheis, Simone Baldini [UNESP] |
dc.subject.por.fl_str_mv |
Dog Cat Diagnosis Leishmaniasis Zoonosis |
topic |
Dog Cat Diagnosis Leishmaniasis Zoonosis |
description |
Background: This study aimed to evaluate the occurrence of Leishmania spp. in dogs and cats from Botucatu, Sao Paulo state, and Campo Grande, Mato Grosso do Sul state, Brazil, by the association of three diagnostic tests: blood culture in liver infusion tryptose medium, immunofluorescent antibody test and polymerase chain reaction. Fifty blood samples of dogs and cats from the Center for Zoonosis Control in Campo Grande, an area endemic for canine visceral leishmaniasis, were collected randomly, as well as canine and feline blood samples from the Municipal Kennel and Animal Protection Association in Botucatu, currently considered a transmission-free, non-endemic area.Results: Of the 50 dog blood cultures from Botucatu, three (6%) were positive and of the 50 cats, two (4%) were positive. In Campo Grande, 29 dog blood cultures (58%) were positive and all (100%) cats negative by this test. Polymerase chain reaction detected Leishmania spp. in 100% of dog and cat samples from Botucatu but found all the cats from Campo Grande to be negative. On the other hand, 36 dogs from Campo Grande were positive (72%) by the same technique. Immunofluorescent antibody test in Botucatu found 100% of dogs and cats non-reactive, while in Campo Grande, it detected positivity in 32 dogs (64%) and 15 cats (30%).Conclusions: The results show the importance of not only continuous epidemiological surveillance in areas not endemic for leishmaniasis, but also research for accurate diagnosis of this zoonosis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:10:38Z 2014-12-03T13:10:38Z 2014-02-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1678-9199-20-5 Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Biomed Central Ltd, v. 20, 7 p., 2014. 1678-9199 http://hdl.handle.net/11449/112346 10.1186/1678-9199-20-5 WOS:000334697100001 WOS000334697100001.pdf 5326072118518067 |
url |
http://dx.doi.org/10.1186/1678-9199-20-5 http://hdl.handle.net/11449/112346 |
identifier_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Biomed Central Ltd, v. 20, 7 p., 2014. 1678-9199 10.1186/1678-9199-20-5 WOS:000334697100001 WOS000334697100001.pdf 5326072118518067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962129447354368 |