Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13578 http://hdl.handle.net/11449/233282 |
Resumo: | Aim: To evaluate the effect of excessive caffeine intake on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Sixteen Wistar rats were used. Periapical periodontitis was induced in the four first molars in each animal. The animals were arranged into two groups: control (C)—rats with periapical periodontitis; and caffeine (CAF)—rats with periapical periodontitis under caffeine administration protocol. The CAF animals received 10 mg/100 g of body weight/day of caffeine via gavage starting fifteen days before PP induction and continuing for thirty more days until euthanasia. On the 30th day, the animals were euthanized and the jaws removed for microcomputed tomography, histological and immunohistochemical analysis for RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β. The Mann–Whitney test was performed for nonparametric data, and Student's t test was performed for parametric data, using p <.05. Results: There was no significant difference in the weight change between the groups. The median score of the inflammatory process was significantly greater in the CAF group (3) compared with the C group (2), p =.0256. Bone resorption was greater in the group consuming caffeine (1.08 ± 0.15 mm3) compared with the C group (0.88 ± 0.10 mm3), p =.0346. The immunolabelling for RANKL, TRAP and IL-1β was significantly higher in the CAF group when compared to the control, p <.05. No differences were found for the OPG, IL-10 and TNF-⍺ immunolabelling. Conclusion: Excessive caffeine exposure via gavage in rats was able to exacerbate the volume of periapical bone destruction, and the inflammatory pattern deriving from periapical periodontitis altering the expression of RANKL, IL-1β and TRAP. |
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Excessive caffeine intake increases bone resorption associated with periapical periodontitis in ratscaffeineendodonticsperiapical periodontitisAim: To evaluate the effect of excessive caffeine intake on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Sixteen Wistar rats were used. Periapical periodontitis was induced in the four first molars in each animal. The animals were arranged into two groups: control (C)—rats with periapical periodontitis; and caffeine (CAF)—rats with periapical periodontitis under caffeine administration protocol. The CAF animals received 10 mg/100 g of body weight/day of caffeine via gavage starting fifteen days before PP induction and continuing for thirty more days until euthanasia. On the 30th day, the animals were euthanized and the jaws removed for microcomputed tomography, histological and immunohistochemical analysis for RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β. The Mann–Whitney test was performed for nonparametric data, and Student's t test was performed for parametric data, using p <.05. Results: There was no significant difference in the weight change between the groups. The median score of the inflammatory process was significantly greater in the CAF group (3) compared with the C group (2), p =.0256. Bone resorption was greater in the group consuming caffeine (1.08 ± 0.15 mm3) compared with the C group (0.88 ± 0.10 mm3), p =.0346. The immunolabelling for RANKL, TRAP and IL-1β was significantly higher in the CAF group when compared to the control, p <.05. No differences were found for the OPG, IL-10 and TNF-⍺ immunolabelling. Conclusion: Excessive caffeine exposure via gavage in rats was able to exacerbate the volume of periapical bone destruction, and the inflammatory pattern deriving from periapical periodontitis altering the expression of RANKL, IL-1β and TRAP.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Preventive and Restorative DentistryDepartment of Basic Science School of Dentistry São Paulo State University (Unesp)Department of Restorative Dentistry and Endodontics School of Dentistry Federal University of Alagoas (UFAL)Department of Basic Science School of Dentistry São Paulo State University (Unesp)FAPESP: 2017/27219-3Universidade Estadual Paulista (UNESP)Federal University of Alagoas (UFAL)Dal-Fabbro, RenanCosme-Silva, LeopoldoCapalbo, Letícia CabreraChaves-Neto, Antonio Hernandes [UNESP]Ervolino, Edilson [UNESP]Cintra, Luciano Tavares AngeloGomes-Filho, João Eduardo2022-05-01T06:02:38Z2022-05-01T06:02:38Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1861-1870http://dx.doi.org/10.1111/iej.13578International Endodontic Journal, v. 54, n. 10, p. 1861-1870, 2021.1365-25910143-2885http://hdl.handle.net/11449/23328210.1111/iej.135782-s2.0-85110595690Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2022-05-01T06:02:38Zoai:repositorio.unesp.br:11449/233282Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:24:47.075656Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
title |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
spellingShingle |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats Dal-Fabbro, Renan caffeine endodontics periapical periodontitis |
title_short |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
title_full |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
title_fullStr |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
title_full_unstemmed |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
title_sort |
Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats |
author |
Dal-Fabbro, Renan |
author_facet |
Dal-Fabbro, Renan Cosme-Silva, Leopoldo Capalbo, Letícia Cabrera Chaves-Neto, Antonio Hernandes [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo Gomes-Filho, João Eduardo |
author_role |
author |
author2 |
Cosme-Silva, Leopoldo Capalbo, Letícia Cabrera Chaves-Neto, Antonio Hernandes [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo Gomes-Filho, João Eduardo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Alagoas (UFAL) |
dc.contributor.author.fl_str_mv |
Dal-Fabbro, Renan Cosme-Silva, Leopoldo Capalbo, Letícia Cabrera Chaves-Neto, Antonio Hernandes [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo Gomes-Filho, João Eduardo |
dc.subject.por.fl_str_mv |
caffeine endodontics periapical periodontitis |
topic |
caffeine endodontics periapical periodontitis |
description |
Aim: To evaluate the effect of excessive caffeine intake on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Sixteen Wistar rats were used. Periapical periodontitis was induced in the four first molars in each animal. The animals were arranged into two groups: control (C)—rats with periapical periodontitis; and caffeine (CAF)—rats with periapical periodontitis under caffeine administration protocol. The CAF animals received 10 mg/100 g of body weight/day of caffeine via gavage starting fifteen days before PP induction and continuing for thirty more days until euthanasia. On the 30th day, the animals were euthanized and the jaws removed for microcomputed tomography, histological and immunohistochemical analysis for RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β. The Mann–Whitney test was performed for nonparametric data, and Student's t test was performed for parametric data, using p <.05. Results: There was no significant difference in the weight change between the groups. The median score of the inflammatory process was significantly greater in the CAF group (3) compared with the C group (2), p =.0256. Bone resorption was greater in the group consuming caffeine (1.08 ± 0.15 mm3) compared with the C group (0.88 ± 0.10 mm3), p =.0346. The immunolabelling for RANKL, TRAP and IL-1β was significantly higher in the CAF group when compared to the control, p <.05. No differences were found for the OPG, IL-10 and TNF-⍺ immunolabelling. Conclusion: Excessive caffeine exposure via gavage in rats was able to exacerbate the volume of periapical bone destruction, and the inflammatory pattern deriving from periapical periodontitis altering the expression of RANKL, IL-1β and TRAP. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-05-01T06:02:38Z 2022-05-01T06:02:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13578 International Endodontic Journal, v. 54, n. 10, p. 1861-1870, 2021. 1365-2591 0143-2885 http://hdl.handle.net/11449/233282 10.1111/iej.13578 2-s2.0-85110595690 |
url |
http://dx.doi.org/10.1111/iej.13578 http://hdl.handle.net/11449/233282 |
identifier_str_mv |
International Endodontic Journal, v. 54, n. 10, p. 1861-1870, 2021. 1365-2591 0143-2885 10.1111/iej.13578 2-s2.0-85110595690 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1861-1870 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128645162598400 |