Phenotypic characterization murine carcoma TG-180 immunophenotypical characterization murine sarcoma TG-180

• Autor(a) principal: Bernardes, Thais Martins Torres [UNESP]
• Data de Publicação: 2015
• Outros Autores: Beserra, Hugo Enrique Orsini [UNESP], Vexenat, Stephane Cássia Oliveira Rosa [UNESP], Langoni, Helio [UNESP], Rocha, Noeme Sousa [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4236/ojpathology.2015.52009; http://hdl.handle.net/11449/140561
• topic: Immunohistochemistry; Neoplasia; Mice; Tumor origin

The sarcoma is the generic nomenclature for neoplasm of mesodermal cells, which express in man and animals. Silent growth requires early diagnosis technique for identifying their proteins. The experimental model in vivo murine sarcoma 180-TG (TG-180), is widely used in research to provide the stimuli of infectious and neoplastic antigens. In this case, the technique of immunohistochemistry helps identify the expressions of tumor cell variants. The objective of the research was to characterize immunoexpression murine sarcoma TG 180, by immunohistochemistry, antibodies AE1/AE3, vimentin, CD3, CD 45, CD79α and S100A4. For this, murine sarcoma TG-180, was implanted subcutaneously in 20 mice “Swiss”, male, 30 days old, 28 g for 10 days. Samples were taken and subjected to immunohistochemistry, via use of HistoMouse-MAX™ kit. There was specifically labeled S100A4 and vimentin antibodies, indicative of poorly differentiated neoplasms fibroblasts. In fact, the model is established by identifying the origin of the cell, once identified, chemotherapeutic tests can also be performed. Neoplasia like these, when installed in man and animals, depending on the degree of aggressiveness requires treatment protocol varied between surgery and chemotherapy or combination of treatments.