Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1519-566X2007000500011 http://hdl.handle.net/11449/69854 |
Resumo: | The silk gland in Lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. The secretion of fibroin and sericin by the different silk gland regions are well established. There are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. This study characterizes the glandular region and detects the presence of lipid components in the secretion of the silk gland of Diatraea saccharalis (Fabricius). The silk gland was submitted to histochemical procedure for lipid detection or conventionally prepared for ultrastructural analyses. Lipid droplets were histochemically detected in both the apical cytoplasm of cell of the anterior region and in the lumen among the microvilli. Ultrastructural analyses of the anterior region showed lipid material, visualized as myelin-like structures within the vesicular Golgi complex and in the apical secretory globules, mixed up with the sericin; similar material was observed into the lumen, adjacent to the microvilli. Lipids were not detected in the cells neither in the lumen of the posterior region. Our results suggest that the silk produced by D. saccharalis has a minor lipid content that is secreted by the anterior region together with the sericin. |
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Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)Evidências histoquímicas e ultra-estruturais de secreção sipídica pela glândula da seda da broca da cana-de-açúcar, Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)InsectLarvaSecretory celllipidanimalexocrine glandhistologylarvaLepidopteraphysiologysecretionsugarcaneultrastructureAnimalsExocrine GlandsLipidsSaccharumCrambidaeDiatraea saccharalisHexapodaThe silk gland in Lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. The secretion of fibroin and sericin by the different silk gland regions are well established. There are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. This study characterizes the glandular region and detects the presence of lipid components in the secretion of the silk gland of Diatraea saccharalis (Fabricius). The silk gland was submitted to histochemical procedure for lipid detection or conventionally prepared for ultrastructural analyses. Lipid droplets were histochemically detected in both the apical cytoplasm of cell of the anterior region and in the lumen among the microvilli. Ultrastructural analyses of the anterior region showed lipid material, visualized as myelin-like structures within the vesicular Golgi complex and in the apical secretory globules, mixed up with the sericin; similar material was observed into the lumen, adjacent to the microvilli. Lipids were not detected in the cells neither in the lumen of the posterior region. Our results suggest that the silk produced by D. saccharalis has a minor lipid content that is secreted by the anterior region together with the sericin.Centro de Microscopia Eletrônica Instituto de Biociências Univ. Estadual Paulista - UNESP, Rubião Júnior, s/n, 18618-000, Botucatu, SPCentro de Microscopia Eletrônica Instituto de Biociências Univ. Estadual Paulista - UNESP, Rubião Júnior, s/n, 18618-000, Botucatu, SPUniversidade Estadual Paulista (Unesp)Victoriano, Eliane [UNESP]Pinheiro, Daniela O. [UNESP]Gregório, Elisa A. [UNESP]2014-05-27T11:22:34Z2014-05-27T11:22:34Z2007-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article707-711application/pdfhttp://dx.doi.org/10.1590/S1519-566X2007000500011Neotropical Entomology, v. 36, n. 5, p. 707-711, 2007.1519-566Xhttp://hdl.handle.net/11449/6985410.1590/S1519-566X2007000500011S1519-566X2007000500011WOS:0002511363000112-s2.0-373490670372-s2.0-37349067037.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeotropical Entomology0.886info:eu-repo/semantics/openAccess2024-04-11T19:33:51Zoai:repositorio.unesp.br:11449/69854Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:38:07.592716Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) Evidências histoquímicas e ultra-estruturais de secreção sipídica pela glândula da seda da broca da cana-de-açúcar, Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
title |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
spellingShingle |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) Victoriano, Eliane [UNESP] Insect Larva Secretory cell lipid animal exocrine gland histology larva Lepidoptera physiology secretion sugarcane ultrastructure Animals Exocrine Glands Lipids Saccharum Crambidae Diatraea saccharalis Hexapoda |
title_short |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
title_full |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
title_fullStr |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
title_full_unstemmed |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
title_sort |
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) |
author |
Victoriano, Eliane [UNESP] |
author_facet |
Victoriano, Eliane [UNESP] Pinheiro, Daniela O. [UNESP] Gregório, Elisa A. [UNESP] |
author_role |
author |
author2 |
Pinheiro, Daniela O. [UNESP] Gregório, Elisa A. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Victoriano, Eliane [UNESP] Pinheiro, Daniela O. [UNESP] Gregório, Elisa A. [UNESP] |
dc.subject.por.fl_str_mv |
Insect Larva Secretory cell lipid animal exocrine gland histology larva Lepidoptera physiology secretion sugarcane ultrastructure Animals Exocrine Glands Lipids Saccharum Crambidae Diatraea saccharalis Hexapoda |
topic |
Insect Larva Secretory cell lipid animal exocrine gland histology larva Lepidoptera physiology secretion sugarcane ultrastructure Animals Exocrine Glands Lipids Saccharum Crambidae Diatraea saccharalis Hexapoda |
description |
The silk gland in Lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. The secretion of fibroin and sericin by the different silk gland regions are well established. There are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. This study characterizes the glandular region and detects the presence of lipid components in the secretion of the silk gland of Diatraea saccharalis (Fabricius). The silk gland was submitted to histochemical procedure for lipid detection or conventionally prepared for ultrastructural analyses. Lipid droplets were histochemically detected in both the apical cytoplasm of cell of the anterior region and in the lumen among the microvilli. Ultrastructural analyses of the anterior region showed lipid material, visualized as myelin-like structures within the vesicular Golgi complex and in the apical secretory globules, mixed up with the sericin; similar material was observed into the lumen, adjacent to the microvilli. Lipids were not detected in the cells neither in the lumen of the posterior region. Our results suggest that the silk produced by D. saccharalis has a minor lipid content that is secreted by the anterior region together with the sericin. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 2014-05-27T11:22:34Z 2014-05-27T11:22:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1519-566X2007000500011 Neotropical Entomology, v. 36, n. 5, p. 707-711, 2007. 1519-566X http://hdl.handle.net/11449/69854 10.1590/S1519-566X2007000500011 S1519-566X2007000500011 WOS:000251136300011 2-s2.0-37349067037 2-s2.0-37349067037.pdf |
url |
http://dx.doi.org/10.1590/S1519-566X2007000500011 http://hdl.handle.net/11449/69854 |
identifier_str_mv |
Neotropical Entomology, v. 36, n. 5, p. 707-711, 2007. 1519-566X 10.1590/S1519-566X2007000500011 S1519-566X2007000500011 WOS:000251136300011 2-s2.0-37349067037 2-s2.0-37349067037.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neotropical Entomology 0.886 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
707-711 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128956869640192 |