β-galactosidase immobilization on controlled pore silica

Detalhes bibliográficos
Autor(a) principal: Trevisan, H. C. [UNESP]
Data de Publicação: 1997
Outros Autores: Bergamo, E. P. [UNESP], Contiero, Jonas [UNESP], Hojo, Ossamu [UNESP], Mont, R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0104-66321997000400003
http://hdl.handle.net/11449/65302
Resumo: The immobilization of β-galactosidase from Kluyveromyces fragilis on controlled pore silica was investigated. Immobilization was performed on amino silica activated with glutaraldehyde and the product was applied to the hydrolysis of lactose of whey. The behaviors of the soluble and immobilized enzyme were compared by using whey and a lactose solution as the substrate. With the aim of optimizing the method, parameters such as the amount of glutaraldehyde and the size of the particles were evaluated by comparing activities and stabilities on batch and continuously fluidized bed reactors.
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spelling β-galactosidase immobilization on controlled pore silicaAldehydesFluidized bedsHydrolysisPorous materialsSilicaSugarsLactosePorous silicaEnzyme immobilizationThe immobilization of β-galactosidase from Kluyveromyces fragilis on controlled pore silica was investigated. Immobilization was performed on amino silica activated with glutaraldehyde and the product was applied to the hydrolysis of lactose of whey. The behaviors of the soluble and immobilized enzyme were compared by using whey and a lactose solution as the substrate. With the aim of optimizing the method, parameters such as the amount of glutaraldehyde and the size of the particles were evaluated by comparing activities and stabilities on batch and continuously fluidized bed reactors.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Unesp, AraraquaraUnesp, AraraquaraUniversidade Estadual Paulista (Unesp)Trevisan, H. C. [UNESP]Bergamo, E. P. [UNESP]Contiero, Jonas [UNESP]Hojo, Ossamu [UNESP]Mont, R. [UNESP]2014-05-27T11:18:19Z2014-05-27T11:18:19Z1997-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article315-319http://dx.doi.org/10.1590/S0104-66321997000400003Brazilian Journal of Chemical Engineering, v. 14, n. 4, p. 315-319, 1997.0104-6632http://hdl.handle.net/11449/6530210.1590/S0104-66321997000400003S0104-663219970004000032-s2.0-0031364163985915497944700578865783262483410000-0003-2289-2401Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Chemical Engineering0.9250,395info:eu-repo/semantics/openAccess2021-10-23T12:10:48Zoai:repositorio.unesp.br:11449/65302Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:37:20.495107Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv β-galactosidase immobilization on controlled pore silica
title β-galactosidase immobilization on controlled pore silica
spellingShingle β-galactosidase immobilization on controlled pore silica
Trevisan, H. C. [UNESP]
Aldehydes
Fluidized beds
Hydrolysis
Porous materials
Silica
Sugars
Lactose
Porous silica
Enzyme immobilization
title_short β-galactosidase immobilization on controlled pore silica
title_full β-galactosidase immobilization on controlled pore silica
title_fullStr β-galactosidase immobilization on controlled pore silica
title_full_unstemmed β-galactosidase immobilization on controlled pore silica
title_sort β-galactosidase immobilization on controlled pore silica
author Trevisan, H. C. [UNESP]
author_facet Trevisan, H. C. [UNESP]
Bergamo, E. P. [UNESP]
Contiero, Jonas [UNESP]
Hojo, Ossamu [UNESP]
Mont, R. [UNESP]
author_role author
author2 Bergamo, E. P. [UNESP]
Contiero, Jonas [UNESP]
Hojo, Ossamu [UNESP]
Mont, R. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Trevisan, H. C. [UNESP]
Bergamo, E. P. [UNESP]
Contiero, Jonas [UNESP]
Hojo, Ossamu [UNESP]
Mont, R. [UNESP]
dc.subject.por.fl_str_mv Aldehydes
Fluidized beds
Hydrolysis
Porous materials
Silica
Sugars
Lactose
Porous silica
Enzyme immobilization
topic Aldehydes
Fluidized beds
Hydrolysis
Porous materials
Silica
Sugars
Lactose
Porous silica
Enzyme immobilization
description The immobilization of β-galactosidase from Kluyveromyces fragilis on controlled pore silica was investigated. Immobilization was performed on amino silica activated with glutaraldehyde and the product was applied to the hydrolysis of lactose of whey. The behaviors of the soluble and immobilized enzyme were compared by using whey and a lactose solution as the substrate. With the aim of optimizing the method, parameters such as the amount of glutaraldehyde and the size of the particles were evaluated by comparing activities and stabilities on batch and continuously fluidized bed reactors.
publishDate 1997
dc.date.none.fl_str_mv 1997-12-01
2014-05-27T11:18:19Z
2014-05-27T11:18:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0104-66321997000400003
Brazilian Journal of Chemical Engineering, v. 14, n. 4, p. 315-319, 1997.
0104-6632
http://hdl.handle.net/11449/65302
10.1590/S0104-66321997000400003
S0104-66321997000400003
2-s2.0-0031364163
9859154979447005
7886578326248341
0000-0003-2289-2401
url http://dx.doi.org/10.1590/S0104-66321997000400003
http://hdl.handle.net/11449/65302
identifier_str_mv Brazilian Journal of Chemical Engineering, v. 14, n. 4, p. 315-319, 1997.
0104-6632
10.1590/S0104-66321997000400003
S0104-66321997000400003
2-s2.0-0031364163
9859154979447005
7886578326248341
0000-0003-2289-2401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Chemical Engineering
0.925
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 315-319
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129228447678464

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