Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol9-3gmr821 http://hdl.handle.net/11449/6534 |
Resumo: | Despite the implementation control programs, schistosomiasis continues to spread throughout the world. Among modern control strategies, vector control is currently being emphasized. Within this context, analysis of the genetic variability of intermediate host snails (Biomphalaria spp) is important because it allows identification of specific sequences of the genome of this mollusk related to susceptibility/resistance to Schistosoma mansoni infection. We investigated Brazilian albino (non-pigmented) and pigmented (wild type) strains of Biomphalaria glabrata; these strains differ in their susceptibility to S. mansoni infection. Genetic variability was studied by RAPD-PCR using different random primers. The electrophoretic patterns resulting from amplification showed specific polymorphic markers for the albino and pigmented strains of B. glabrata. This information will help in the identification and isolation of genes specifically related to susceptibility, demonstrating that RAPD-PCR is an appropriate and efficient methodological approach for analysis of the genetic variability of schistosomiasis vectors. |
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Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasisSchistosoma mansoniBiomphalariaPolymorphismGenetic variabilityRAPD-PCRDespite the implementation control programs, schistosomiasis continues to spread throughout the world. Among modern control strategies, vector control is currently being emphasized. Within this context, analysis of the genetic variability of intermediate host snails (Biomphalaria spp) is important because it allows identification of specific sequences of the genome of this mollusk related to susceptibility/resistance to Schistosoma mansoni infection. We investigated Brazilian albino (non-pigmented) and pigmented (wild type) strains of Biomphalaria glabrata; these strains differ in their susceptibility to S. mansoni infection. Genetic variability was studied by RAPD-PCR using different random primers. The electrophoretic patterns resulting from amplification showed specific polymorphic markers for the albino and pigmented strains of B. glabrata. This information will help in the identification and isolation of genes specifically related to susceptibility, demonstrating that RAPD-PCR is an appropriate and efficient methodological approach for analysis of the genetic variability of schistosomiasis vectors.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Ciencias Biol, Assis, SP, BrazilUniv São Paulo, Interunidades Biotecnol, São Paulo, BrazilUniv Estadual Campinas, Dept Parasitol, Inst Biol, Campinas, SP, BrazilADR Biomavale, Dept Saude, Assis, SP, BrazilUniv Estadual Paulista, Dept Ciencias Saude, Assis, SP, BrazilNatl Res Ctr, Dept Med Chem, Cairo, EgyptUniv Estadual Paulista, Dept Ciencias Biol, Assis, SP, BrazilUniv Estadual Paulista, Dept Ciencias Saude, Assis, SP, BrazilFunpec-editoraUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)ADR BiomavaleNatl Res CtrOliveira, A. L. D. [UNESP]Da Silva, D. [UNESP]Manzano, B. C. [UNESP]Abdel-Hamid, A. Z.Marcelino, M. Y.Zanotti-Magalhaes, E. M.Magalhaes, L. A.Ribeiro-Paes, J. T. [UNESP]2014-05-20T13:22:19Z2014-05-20T13:22:19Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1450-1459application/pdfhttp://dx.doi.org/10.4238/vol9-3gmr821Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 3, p. 1450-1459, 2010.1676-5680http://hdl.handle.net/11449/653410.4238/vol9-3gmr821WOS:000280397000019WOS000280397000019.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2024-06-13T17:39:05Zoai:repositorio.unesp.br:11449/6534Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:10:19.713245Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
title |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
spellingShingle |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis Oliveira, A. L. D. [UNESP] Schistosoma mansoni Biomphalaria Polymorphism Genetic variability RAPD-PCR |
title_short |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
title_full |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
title_fullStr |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
title_full_unstemmed |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
title_sort |
Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis |
author |
Oliveira, A. L. D. [UNESP] |
author_facet |
Oliveira, A. L. D. [UNESP] Da Silva, D. [UNESP] Manzano, B. C. [UNESP] Abdel-Hamid, A. Z. Marcelino, M. Y. Zanotti-Magalhaes, E. M. Magalhaes, L. A. Ribeiro-Paes, J. T. [UNESP] |
author_role |
author |
author2 |
Da Silva, D. [UNESP] Manzano, B. C. [UNESP] Abdel-Hamid, A. Z. Marcelino, M. Y. Zanotti-Magalhaes, E. M. Magalhaes, L. A. Ribeiro-Paes, J. T. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) ADR Biomavale Natl Res Ctr |
dc.contributor.author.fl_str_mv |
Oliveira, A. L. D. [UNESP] Da Silva, D. [UNESP] Manzano, B. C. [UNESP] Abdel-Hamid, A. Z. Marcelino, M. Y. Zanotti-Magalhaes, E. M. Magalhaes, L. A. Ribeiro-Paes, J. T. [UNESP] |
dc.subject.por.fl_str_mv |
Schistosoma mansoni Biomphalaria Polymorphism Genetic variability RAPD-PCR |
topic |
Schistosoma mansoni Biomphalaria Polymorphism Genetic variability RAPD-PCR |
description |
Despite the implementation control programs, schistosomiasis continues to spread throughout the world. Among modern control strategies, vector control is currently being emphasized. Within this context, analysis of the genetic variability of intermediate host snails (Biomphalaria spp) is important because it allows identification of specific sequences of the genome of this mollusk related to susceptibility/resistance to Schistosoma mansoni infection. We investigated Brazilian albino (non-pigmented) and pigmented (wild type) strains of Biomphalaria glabrata; these strains differ in their susceptibility to S. mansoni infection. Genetic variability was studied by RAPD-PCR using different random primers. The electrophoretic patterns resulting from amplification showed specific polymorphic markers for the albino and pigmented strains of B. glabrata. This information will help in the identification and isolation of genes specifically related to susceptibility, demonstrating that RAPD-PCR is an appropriate and efficient methodological approach for analysis of the genetic variability of schistosomiasis vectors. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2014-05-20T13:22:19Z 2014-05-20T13:22:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol9-3gmr821 Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 3, p. 1450-1459, 2010. 1676-5680 http://hdl.handle.net/11449/6534 10.4238/vol9-3gmr821 WOS:000280397000019 WOS000280397000019.pdf |
url |
http://dx.doi.org/10.4238/vol9-3gmr821 http://hdl.handle.net/11449/6534 |
identifier_str_mv |
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 9, n. 3, p. 1450-1459, 2010. 1676-5680 10.4238/vol9-3gmr821 WOS:000280397000019 WOS000280397000019.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1450-1459 application/pdf |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129591900897280 |