Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/conphys/coz079 http://hdl.handle.net/11449/198952 |
Resumo: | Blood glucose is widely used as a physiological parameter for vertebrates and invertebrates. However, its measurement in the field is often difficult due to the need for expensive and non-portable equipment. Point-of-care (POC) devices, originally intended for human use, are increasingly being used for measuring blood parameters of animals in the field. In this regard, POC glucose meters are becoming valuable tools for conservation physiologists, as glucose can be a useful indicator of stress response. In invertebrates, the use of POC glucose meters is still scarce, and no study yet has evaluated their usability in crustaceans and molluscs.We tested if a POC device can be used to measure haemolymph glucose in two widely used models, Leptuca thayeri and Perna perna, compared with a standard laboratory method. The device was unable to measure glucose in P. perna haemolymph due to equipment inaccuracy and low glucose concentration in this species (10.13±6.25 mg/dL). Additionally, despite the device being capable of measuring glucose in L. thayeri haemolymph, Bland-Altman plots showed a strong bias and wide limits of agreement, and Lin's concordance correlation coefficient showed a weak concordance between methods. When simulating experimental conditions, POC results differed from those found using the standard method. We conclude that POC glucose meters are unsuitable for assessing glucose in mussels and should not be used in crabs as results are inaccurate. |
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Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethodFiddler crabGlucoseLeptuca thayeriMolluscsPerna pernaPortable meterBlood glucose is widely used as a physiological parameter for vertebrates and invertebrates. However, its measurement in the field is often difficult due to the need for expensive and non-portable equipment. Point-of-care (POC) devices, originally intended for human use, are increasingly being used for measuring blood parameters of animals in the field. In this regard, POC glucose meters are becoming valuable tools for conservation physiologists, as glucose can be a useful indicator of stress response. In invertebrates, the use of POC glucose meters is still scarce, and no study yet has evaluated their usability in crustaceans and molluscs.We tested if a POC device can be used to measure haemolymph glucose in two widely used models, Leptuca thayeri and Perna perna, compared with a standard laboratory method. The device was unable to measure glucose in P. perna haemolymph due to equipment inaccuracy and low glucose concentration in this species (10.13±6.25 mg/dL). Additionally, despite the device being capable of measuring glucose in L. thayeri haemolymph, Bland-Altman plots showed a strong bias and wide limits of agreement, and Lin's concordance correlation coefficient showed a weak concordance between methods. When simulating experimental conditions, POC results differed from those found using the standard method. We conclude that POC glucose meters are unsuitable for assessing glucose in mussels and should not be used in crabs as results are inaccurate.São Paulo State University (UNESP) Biosciences Institute Botucatu Campus, R. Prof. Dr. Antônio Celso 250São Paulo State University (UNESP) Biosciences Institute Coastal Campus, Praça Infante Dom Henrique s/nSão Paulo State University (UNESP) CAUNESP Prof. Paulo Donato Castellane S/nSão Paulo State University (UNESP) Biosciences Institute Botucatu Campus, R. Prof. Dr. Antônio Celso 250São Paulo State University (UNESP) Biosciences Institute Coastal Campus, Praça Infante Dom Henrique s/nSão Paulo State University (UNESP) CAUNESP Prof. Paulo Donato Castellane S/nUniversidade Estadual Paulista (Unesp)Principe, Silas C. [UNESP]Augusto, Alessandra [UNESP]Costa, Tânia M. [UNESP]2020-12-12T01:26:28Z2020-12-12T01:26:28Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/conphys/coz079Conservation Physiology, v. 7, n. 1, 2019.2051-1434http://hdl.handle.net/11449/19895210.1093/conphys/coz0792-s2.0-85086044517Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengConservation Physiologyinfo:eu-repo/semantics/openAccess2021-10-22T21:10:10Zoai:repositorio.unesp.br:11449/198952Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:38:58.084395Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
title |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
spellingShingle |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod Principe, Silas C. [UNESP] Fiddler crab Glucose Leptuca thayeri Molluscs Perna perna Portable meter |
title_short |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
title_full |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
title_fullStr |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
title_full_unstemmed |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
title_sort |
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a validmethod |
author |
Principe, Silas C. [UNESP] |
author_facet |
Principe, Silas C. [UNESP] Augusto, Alessandra [UNESP] Costa, Tânia M. [UNESP] |
author_role |
author |
author2 |
Augusto, Alessandra [UNESP] Costa, Tânia M. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Principe, Silas C. [UNESP] Augusto, Alessandra [UNESP] Costa, Tânia M. [UNESP] |
dc.subject.por.fl_str_mv |
Fiddler crab Glucose Leptuca thayeri Molluscs Perna perna Portable meter |
topic |
Fiddler crab Glucose Leptuca thayeri Molluscs Perna perna Portable meter |
description |
Blood glucose is widely used as a physiological parameter for vertebrates and invertebrates. However, its measurement in the field is often difficult due to the need for expensive and non-portable equipment. Point-of-care (POC) devices, originally intended for human use, are increasingly being used for measuring blood parameters of animals in the field. In this regard, POC glucose meters are becoming valuable tools for conservation physiologists, as glucose can be a useful indicator of stress response. In invertebrates, the use of POC glucose meters is still scarce, and no study yet has evaluated their usability in crustaceans and molluscs.We tested if a POC device can be used to measure haemolymph glucose in two widely used models, Leptuca thayeri and Perna perna, compared with a standard laboratory method. The device was unable to measure glucose in P. perna haemolymph due to equipment inaccuracy and low glucose concentration in this species (10.13±6.25 mg/dL). Additionally, despite the device being capable of measuring glucose in L. thayeri haemolymph, Bland-Altman plots showed a strong bias and wide limits of agreement, and Lin's concordance correlation coefficient showed a weak concordance between methods. When simulating experimental conditions, POC results differed from those found using the standard method. We conclude that POC glucose meters are unsuitable for assessing glucose in mussels and should not be used in crabs as results are inaccurate. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-12T01:26:28Z 2020-12-12T01:26:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/conphys/coz079 Conservation Physiology, v. 7, n. 1, 2019. 2051-1434 http://hdl.handle.net/11449/198952 10.1093/conphys/coz079 2-s2.0-85086044517 |
url |
http://dx.doi.org/10.1093/conphys/coz079 http://hdl.handle.net/11449/198952 |
identifier_str_mv |
Conservation Physiology, v. 7, n. 1, 2019. 2051-1434 10.1093/conphys/coz079 2-s2.0-85086044517 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Conservation Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129231288270848 |