Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis

Detalhes bibliográficos
Autor(a) principal: Rivera-Calderón, Luis Gabriel [UNESP]
Data de Publicação: 2016
Outros Autores: Fonseca-Alves, Carlos Eduardo [UNESP], Kobayashi, Priscila Emiko [UNESP], Carvalho, Marcio [UNESP], Drigo, Sandra Aparecida [UNESP], de Oliveira Vasconcelos, Rosemeri [UNESP], Laufer-Amorim, Renée [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.rvsc.2016.03.008
http://hdl.handle.net/11449/172719
Resumo: The PTEN, AR, MDM2 and p53 protein network plays a central role in the development of many human cancers, thus eliciting the development of targeted cancer therapeutics. Dogs spontaneously develop tumours, and they are considered a good model for comparative oncology initiatives. Due to the limited information on these proteins in canine tumours, this study aimed to investigate gene and protein alterations in PTEN, AR, MDM2 and p53 in canine prostate cancer (PC). Protein expression was evaluated by immunohistochemistry (15 normal, 22 proliferative inflammatory atrophy (PIA) and 19 PC samples) and Western blotting (2 normal prostate tissue, 2 BPH, 2 PIA samples and 2 PC samples) and gene expression by RT-qPCR (10 normal, 10 PIA and 15 PC samples) of formalin-fixed tissue. We identified nuclear and cytoplasmic expression of PTEN and p53 in all samples, with only nuclear staining found for MDM2 and AR. Our results revealed high expression of MDM2 in PC and PIA samples compared to normal samples, whereas PTEN, P53 and AR expression was down-regulated in PC compared to normal tissue. All tumour samples (n = 19) showed loss of nuclear PTEN expression, and all cancer mimickers showed positive nuclear staining. Therefore, nuclear PTEN staining could be a good diagnostic marker for differentiating between malignant lesions and mimickers. Canine prostate carcinogenesis involves increased expression of MDM2 in association with decreased expression of PTEN, p53 and AR, such as occurs in hormone refractory PC in men. Thus, dogs may be an important model for studying advanced stage PC.
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spelling Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesisDogFormalin-fixed tissueGene expressionImmunohistochemistryProstatic diseaseThe PTEN, AR, MDM2 and p53 protein network plays a central role in the development of many human cancers, thus eliciting the development of targeted cancer therapeutics. Dogs spontaneously develop tumours, and they are considered a good model for comparative oncology initiatives. Due to the limited information on these proteins in canine tumours, this study aimed to investigate gene and protein alterations in PTEN, AR, MDM2 and p53 in canine prostate cancer (PC). Protein expression was evaluated by immunohistochemistry (15 normal, 22 proliferative inflammatory atrophy (PIA) and 19 PC samples) and Western blotting (2 normal prostate tissue, 2 BPH, 2 PIA samples and 2 PC samples) and gene expression by RT-qPCR (10 normal, 10 PIA and 15 PC samples) of formalin-fixed tissue. We identified nuclear and cytoplasmic expression of PTEN and p53 in all samples, with only nuclear staining found for MDM2 and AR. Our results revealed high expression of MDM2 in PC and PIA samples compared to normal samples, whereas PTEN, P53 and AR expression was down-regulated in PC compared to normal tissue. All tumour samples (n = 19) showed loss of nuclear PTEN expression, and all cancer mimickers showed positive nuclear staining. Therefore, nuclear PTEN staining could be a good diagnostic marker for differentiating between malignant lesions and mimickers. Canine prostate carcinogenesis involves increased expression of MDM2 in association with decreased expression of PTEN, p53 and AR, such as occurs in hormone refractory PC in men. Thus, dogs may be an important model for studying advanced stage PC.Faculdade de Ciências Agronômicas Univ. Estadual Paulista - UNESPDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science Univ. Estadual Paulista - UNESPDepartment of Urology Botucatu Medical School - FMB Univ. Estadual Paulista - UNESPFaculdade de Ciências Agronômicas Univ. Estadual Paulista - UNESPDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science Univ. Estadual Paulista - UNESPDepartment of Urology Botucatu Medical School - FMB Univ. Estadual Paulista - UNESPUniversidade Estadual Paulista (Unesp)Rivera-Calderón, Luis Gabriel [UNESP]Fonseca-Alves, Carlos Eduardo [UNESP]Kobayashi, Priscila Emiko [UNESP]Carvalho, Marcio [UNESP]Drigo, Sandra Aparecida [UNESP]de Oliveira Vasconcelos, Rosemeri [UNESP]Laufer-Amorim, Renée [UNESP]2018-12-11T17:01:54Z2018-12-11T17:01:54Z2016-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article56-61application/pdfhttp://dx.doi.org/10.1016/j.rvsc.2016.03.008Research in Veterinary Science, v. 106, p. 56-61.1532-26610034-5288http://hdl.handle.net/11449/17271910.1016/j.rvsc.2016.03.0082-s2.0-849612562362-s2.0-84961256236.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Science0,593info:eu-repo/semantics/openAccess2024-09-03T14:29:56Zoai:repositorio.unesp.br:11449/172719Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
title Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
spellingShingle Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
Rivera-Calderón, Luis Gabriel [UNESP]
Dog
Formalin-fixed tissue
Gene expression
Immunohistochemistry
Prostatic disease
title_short Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
title_full Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
title_fullStr Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
title_full_unstemmed Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
title_sort Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis
author Rivera-Calderón, Luis Gabriel [UNESP]
author_facet Rivera-Calderón, Luis Gabriel [UNESP]
Fonseca-Alves, Carlos Eduardo [UNESP]
Kobayashi, Priscila Emiko [UNESP]
Carvalho, Marcio [UNESP]
Drigo, Sandra Aparecida [UNESP]
de Oliveira Vasconcelos, Rosemeri [UNESP]
Laufer-Amorim, Renée [UNESP]
author_role author
author2 Fonseca-Alves, Carlos Eduardo [UNESP]
Kobayashi, Priscila Emiko [UNESP]
Carvalho, Marcio [UNESP]
Drigo, Sandra Aparecida [UNESP]
de Oliveira Vasconcelos, Rosemeri [UNESP]
Laufer-Amorim, Renée [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rivera-Calderón, Luis Gabriel [UNESP]
Fonseca-Alves, Carlos Eduardo [UNESP]
Kobayashi, Priscila Emiko [UNESP]
Carvalho, Marcio [UNESP]
Drigo, Sandra Aparecida [UNESP]
de Oliveira Vasconcelos, Rosemeri [UNESP]
Laufer-Amorim, Renée [UNESP]
dc.subject.por.fl_str_mv Dog
Formalin-fixed tissue
Gene expression
Immunohistochemistry
Prostatic disease
topic Dog
Formalin-fixed tissue
Gene expression
Immunohistochemistry
Prostatic disease
description The PTEN, AR, MDM2 and p53 protein network plays a central role in the development of many human cancers, thus eliciting the development of targeted cancer therapeutics. Dogs spontaneously develop tumours, and they are considered a good model for comparative oncology initiatives. Due to the limited information on these proteins in canine tumours, this study aimed to investigate gene and protein alterations in PTEN, AR, MDM2 and p53 in canine prostate cancer (PC). Protein expression was evaluated by immunohistochemistry (15 normal, 22 proliferative inflammatory atrophy (PIA) and 19 PC samples) and Western blotting (2 normal prostate tissue, 2 BPH, 2 PIA samples and 2 PC samples) and gene expression by RT-qPCR (10 normal, 10 PIA and 15 PC samples) of formalin-fixed tissue. We identified nuclear and cytoplasmic expression of PTEN and p53 in all samples, with only nuclear staining found for MDM2 and AR. Our results revealed high expression of MDM2 in PC and PIA samples compared to normal samples, whereas PTEN, P53 and AR expression was down-regulated in PC compared to normal tissue. All tumour samples (n = 19) showed loss of nuclear PTEN expression, and all cancer mimickers showed positive nuclear staining. Therefore, nuclear PTEN staining could be a good diagnostic marker for differentiating between malignant lesions and mimickers. Canine prostate carcinogenesis involves increased expression of MDM2 in association with decreased expression of PTEN, p53 and AR, such as occurs in hormone refractory PC in men. Thus, dogs may be an important model for studying advanced stage PC.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-01
2018-12-11T17:01:54Z
2018-12-11T17:01:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.rvsc.2016.03.008
Research in Veterinary Science, v. 106, p. 56-61.
1532-2661
0034-5288
http://hdl.handle.net/11449/172719
10.1016/j.rvsc.2016.03.008
2-s2.0-84961256236
2-s2.0-84961256236.pdf
url http://dx.doi.org/10.1016/j.rvsc.2016.03.008
http://hdl.handle.net/11449/172719
identifier_str_mv Research in Veterinary Science, v. 106, p. 56-61.
1532-2661
0034-5288
10.1016/j.rvsc.2016.03.008
2-s2.0-84961256236
2-s2.0-84961256236.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Research in Veterinary Science
0,593
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 56-61
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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