Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fonc.2022.914156 http://hdl.handle.net/11449/241691 |
Resumo: | Integrins are heterodimeric transmembrane glycoproteins resulting from the non-covalent association of an α and β chain. The major integrin receptor for collagen/laminin, α2β1 is expressed on a wide variety of cell types and plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Integrin-triggered signaling pathways promote the invasion and survival of glioma cells by modifying the brain microenvironment. In this study, we investigated the association of a specific genetic polymorphism of integrin α2β1 with the incidence of diffusely infiltrating astrocytoma and the progression of these tumors. Single-nucleotide polymorphism in intron 7 of the integrin ITGA2 gene was examined in 158 patients and 162 controls using polymerase chain reaction and restriction enzyme analysis. The ITGA2 genotype +/+ (with a BglII restriction site in both alleles) exhibited higher frequency in grade II astrocytoma compared to control (P = 0.02) whereas the genotype -/- (lacking the BglII site) correlated with the poorest survival rate (P = 0.04). In addition, in silico analyses of ITGA2 expression from low-grade gliomas (LGG, n = 515) and glioblastomas (GBM, n = 159) indicated that the higher expression of ITGA2 in LGG was associated with poor overall survival (P < 0.0001). However, the distribution of integrin ITGA2 BglII genotypes (+/+, +/-, -/-) was not significantly different between astrocytoma subgroups III and IV (P = 0.65, 0.24 and 0.33; 0.29, 0.48, 0.25, respectively) compared to control. These results suggest a narrow association between the presence of this SNP and indicate that further studies with larger samples are warranted to analyze the relation between tumor grade and overall survival, highlighting the importance of determining these polymorphisms for prognosis of astrocytomas. |
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Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patientsbrain microenvironmentextracellular matrixinvasionITGA2low grade gliomasingle nucleotide polymorphismtumor progressionIntegrins are heterodimeric transmembrane glycoproteins resulting from the non-covalent association of an α and β chain. The major integrin receptor for collagen/laminin, α2β1 is expressed on a wide variety of cell types and plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Integrin-triggered signaling pathways promote the invasion and survival of glioma cells by modifying the brain microenvironment. In this study, we investigated the association of a specific genetic polymorphism of integrin α2β1 with the incidence of diffusely infiltrating astrocytoma and the progression of these tumors. Single-nucleotide polymorphism in intron 7 of the integrin ITGA2 gene was examined in 158 patients and 162 controls using polymerase chain reaction and restriction enzyme analysis. The ITGA2 genotype +/+ (with a BglII restriction site in both alleles) exhibited higher frequency in grade II astrocytoma compared to control (P = 0.02) whereas the genotype -/- (lacking the BglII site) correlated with the poorest survival rate (P = 0.04). In addition, in silico analyses of ITGA2 expression from low-grade gliomas (LGG, n = 515) and glioblastomas (GBM, n = 159) indicated that the higher expression of ITGA2 in LGG was associated with poor overall survival (P < 0.0001). However, the distribution of integrin ITGA2 BglII genotypes (+/+, +/-, -/-) was not significantly different between astrocytoma subgroups III and IV (P = 0.65, 0.24 and 0.33; 0.29, 0.48, 0.25, respectively) compared to control. These results suggest a narrow association between the presence of this SNP and indicate that further studies with larger samples are warranted to analyze the relation between tumor grade and overall survival, highlighting the importance of determining these polymorphisms for prognosis of astrocytomas.Department of Surgery and Anatomy Ribeirão Preto Medical School University of São PauloMolecular Oncology Research Center Barretos Cancer Hospital, São PauloDepartment of Clinical Toxicological and Bromatological Analysis University of São Paulo Faculty of Pharmaceutical Sciences of Ribeirão PretoDepartment of Neurosurgery Brigham and Women’s Hospital and Harvard Medical SchoolDepartment of Neuroscience and Physiology SUNY Upstate Medical UniversityDepartment of Pathology School of Medicine UNESP- Univ. Estadual PaulistaBarretos School of Health Sciences, Dr. Paulo Prata - FACISBDepartment of Neurology Medical School University of São PauloDepartment of Neurology Faculty of Medicine Federal University of São PauloDepartment of Internal Medicine Faculty of Medicine University of São PauloDepartment of Human Genetics McGill UniversityDepartment of Pathology School of Medicine UNESP- Univ. Estadual PaulistaUniversidade de São Paulo (USP)Barretos Cancer HospitalBrigham and Women’s Hospital and Harvard Medical SchoolSUNY Upstate Medical UniversityUniversidade Estadual Paulista (UNESP)Barretos School of Health SciencesMcGill UniversityTeixeira, Silvia A.Burim, Regislaine V.Viapiano, Mariano S.Bidinotto, Lucas T. [UNESP]Nagashi Marie, Suely K.Fleury Malheiros, Suzana M.Oba-Shinjo, Sueli M.Andrade, Augusto F.Carlotti, Carlos G.2023-03-01T21:17:02Z2023-03-01T21:17:02Z2022-07-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fonc.2022.914156Frontiers in Oncology, v. 12.2234-943Xhttp://hdl.handle.net/11449/24169110.3389/fonc.2022.9141562-s2.0-85135487588Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Oncologyinfo:eu-repo/semantics/openAccess2024-08-16T15:45:52Zoai:repositorio.unesp.br:11449/241691Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T15:45:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
title |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
spellingShingle |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients Teixeira, Silvia A. brain microenvironment extracellular matrix invasion ITGA2 low grade glioma single nucleotide polymorphism tumor progression |
title_short |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
title_full |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
title_fullStr |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
title_full_unstemmed |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
title_sort |
Alpha2beta1 Integrin Polymorphism in Diffuse Astrocytoma Patients |
author |
Teixeira, Silvia A. |
author_facet |
Teixeira, Silvia A. Burim, Regislaine V. Viapiano, Mariano S. Bidinotto, Lucas T. [UNESP] Nagashi Marie, Suely K. Fleury Malheiros, Suzana M. Oba-Shinjo, Sueli M. Andrade, Augusto F. Carlotti, Carlos G. |
author_role |
author |
author2 |
Burim, Regislaine V. Viapiano, Mariano S. Bidinotto, Lucas T. [UNESP] Nagashi Marie, Suely K. Fleury Malheiros, Suzana M. Oba-Shinjo, Sueli M. Andrade, Augusto F. Carlotti, Carlos G. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Barretos Cancer Hospital Brigham and Women’s Hospital and Harvard Medical School SUNY Upstate Medical University Universidade Estadual Paulista (UNESP) Barretos School of Health Sciences McGill University |
dc.contributor.author.fl_str_mv |
Teixeira, Silvia A. Burim, Regislaine V. Viapiano, Mariano S. Bidinotto, Lucas T. [UNESP] Nagashi Marie, Suely K. Fleury Malheiros, Suzana M. Oba-Shinjo, Sueli M. Andrade, Augusto F. Carlotti, Carlos G. |
dc.subject.por.fl_str_mv |
brain microenvironment extracellular matrix invasion ITGA2 low grade glioma single nucleotide polymorphism tumor progression |
topic |
brain microenvironment extracellular matrix invasion ITGA2 low grade glioma single nucleotide polymorphism tumor progression |
description |
Integrins are heterodimeric transmembrane glycoproteins resulting from the non-covalent association of an α and β chain. The major integrin receptor for collagen/laminin, α2β1 is expressed on a wide variety of cell types and plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Integrin-triggered signaling pathways promote the invasion and survival of glioma cells by modifying the brain microenvironment. In this study, we investigated the association of a specific genetic polymorphism of integrin α2β1 with the incidence of diffusely infiltrating astrocytoma and the progression of these tumors. Single-nucleotide polymorphism in intron 7 of the integrin ITGA2 gene was examined in 158 patients and 162 controls using polymerase chain reaction and restriction enzyme analysis. The ITGA2 genotype +/+ (with a BglII restriction site in both alleles) exhibited higher frequency in grade II astrocytoma compared to control (P = 0.02) whereas the genotype -/- (lacking the BglII site) correlated with the poorest survival rate (P = 0.04). In addition, in silico analyses of ITGA2 expression from low-grade gliomas (LGG, n = 515) and glioblastomas (GBM, n = 159) indicated that the higher expression of ITGA2 in LGG was associated with poor overall survival (P < 0.0001). However, the distribution of integrin ITGA2 BglII genotypes (+/+, +/-, -/-) was not significantly different between astrocytoma subgroups III and IV (P = 0.65, 0.24 and 0.33; 0.29, 0.48, 0.25, respectively) compared to control. These results suggest a narrow association between the presence of this SNP and indicate that further studies with larger samples are warranted to analyze the relation between tumor grade and overall survival, highlighting the importance of determining these polymorphisms for prognosis of astrocytomas. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-22 2023-03-01T21:17:02Z 2023-03-01T21:17:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fonc.2022.914156 Frontiers in Oncology, v. 12. 2234-943X http://hdl.handle.net/11449/241691 10.3389/fonc.2022.914156 2-s2.0-85135487588 |
url |
http://dx.doi.org/10.3389/fonc.2022.914156 http://hdl.handle.net/11449/241691 |
identifier_str_mv |
Frontiers in Oncology, v. 12. 2234-943X 10.3389/fonc.2022.914156 2-s2.0-85135487588 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Oncology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128158165106688 |