Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.24425/jppr.2021.137029 http://hdl.handle.net/11449/221856 |
Resumo: | Plants can recognize molecules derived from pathogens and trigger systemic acquired resistance (SAR). In phytopathogenic bacteria, elicitors are constituent components of cellular structures, such as flagellin. We sought to select structural components of Xanthomonas spp. incompatible with tomato, aiming to control bacterial spot (Xanthomonas perforans). Initially, cell suspensions from 11 Xanthomonas spp. isolates were infiltrated into the leaves to assess their ability to cause a hypersensitivity response (HR) and the incompatible ones had their flagellin purified. The flagellin of the isolates were first applied at different concentrations, via infiltration and spraying. The pathogen, X. perforans, was inoculated after 24 h, to assess whether there would be any harmful reaction. No harmful reaction was observed in any treatment. Then, a second experiment was conducted to assess the severity of all isolates, at a concentration of 8.35 μg · ml–1, via spraying, infiltration, and soil. The greatest reduction in Area Under the Disease Progress Curve (AUDPC) was observed in the treatment with XapRR, applied via spraying. Thus, prospecting for elicitors is the first step in developing a product for agricultural use. The flagellin elicitor of XapRR is promising and capable of producing these molecules on a large scale. |
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Prospecting of pathogen-derived elicitors for the control of tomato bacterial spotHypersensitivity responseIncompatibilityPhytopathogenic bacteriaXanthomonas euvesicatoria pv. perforansPlants can recognize molecules derived from pathogens and trigger systemic acquired resistance (SAR). In phytopathogenic bacteria, elicitors are constituent components of cellular structures, such as flagellin. We sought to select structural components of Xanthomonas spp. incompatible with tomato, aiming to control bacterial spot (Xanthomonas perforans). Initially, cell suspensions from 11 Xanthomonas spp. isolates were infiltrated into the leaves to assess their ability to cause a hypersensitivity response (HR) and the incompatible ones had their flagellin purified. The flagellin of the isolates were first applied at different concentrations, via infiltration and spraying. The pathogen, X. perforans, was inoculated after 24 h, to assess whether there would be any harmful reaction. No harmful reaction was observed in any treatment. Then, a second experiment was conducted to assess the severity of all isolates, at a concentration of 8.35 μg · ml–1, via spraying, infiltration, and soil. The greatest reduction in Area Under the Disease Progress Curve (AUDPC) was observed in the treatment with XapRR, applied via spraying. Thus, prospecting for elicitors is the first step in developing a product for agricultural use. The flagellin elicitor of XapRR is promising and capable of producing these molecules on a large scale.Faculdade de Ciências Agronômicas Universidade Estadual Paulita “Júlio de Mesquita Filho”Fitopatologia Embrapa Meio Ambiente, JaguariúnaFaculdade de Ciências Agronômicas Universidade Estadual Paulita “Júlio de Mesquita Filho”Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Simões, Camila Tonelotti [UNESP]Carvalho, Valdeir Nunes [UNESP]de Almeida Halfeld-Vieira, Bernardo2022-04-28T19:40:58Z2022-04-28T19:40:58Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article183-188http://dx.doi.org/10.24425/jppr.2021.137029Journal of Plant Protection Research, v. 61, n. 2, p. 183-188, 2021.1899-007X1427-4345http://hdl.handle.net/11449/22185610.24425/jppr.2021.1370292-s2.0-85108832718Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Protection Researchinfo:eu-repo/semantics/openAccess2022-04-28T19:40:58Zoai:repositorio.unesp.br:11449/221856Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:33:37.667665Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
title |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
spellingShingle |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot Simões, Camila Tonelotti [UNESP] Hypersensitivity response Incompatibility Phytopathogenic bacteria Xanthomonas euvesicatoria pv. perforans |
title_short |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
title_full |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
title_fullStr |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
title_full_unstemmed |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
title_sort |
Prospecting of pathogen-derived elicitors for the control of tomato bacterial spot |
author |
Simões, Camila Tonelotti [UNESP] |
author_facet |
Simões, Camila Tonelotti [UNESP] Carvalho, Valdeir Nunes [UNESP] de Almeida Halfeld-Vieira, Bernardo |
author_role |
author |
author2 |
Carvalho, Valdeir Nunes [UNESP] de Almeida Halfeld-Vieira, Bernardo |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Simões, Camila Tonelotti [UNESP] Carvalho, Valdeir Nunes [UNESP] de Almeida Halfeld-Vieira, Bernardo |
dc.subject.por.fl_str_mv |
Hypersensitivity response Incompatibility Phytopathogenic bacteria Xanthomonas euvesicatoria pv. perforans |
topic |
Hypersensitivity response Incompatibility Phytopathogenic bacteria Xanthomonas euvesicatoria pv. perforans |
description |
Plants can recognize molecules derived from pathogens and trigger systemic acquired resistance (SAR). In phytopathogenic bacteria, elicitors are constituent components of cellular structures, such as flagellin. We sought to select structural components of Xanthomonas spp. incompatible with tomato, aiming to control bacterial spot (Xanthomonas perforans). Initially, cell suspensions from 11 Xanthomonas spp. isolates were infiltrated into the leaves to assess their ability to cause a hypersensitivity response (HR) and the incompatible ones had their flagellin purified. The flagellin of the isolates were first applied at different concentrations, via infiltration and spraying. The pathogen, X. perforans, was inoculated after 24 h, to assess whether there would be any harmful reaction. No harmful reaction was observed in any treatment. Then, a second experiment was conducted to assess the severity of all isolates, at a concentration of 8.35 μg · ml–1, via spraying, infiltration, and soil. The greatest reduction in Area Under the Disease Progress Curve (AUDPC) was observed in the treatment with XapRR, applied via spraying. Thus, prospecting for elicitors is the first step in developing a product for agricultural use. The flagellin elicitor of XapRR is promising and capable of producing these molecules on a large scale. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:40:58Z 2022-04-28T19:40:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.24425/jppr.2021.137029 Journal of Plant Protection Research, v. 61, n. 2, p. 183-188, 2021. 1899-007X 1427-4345 http://hdl.handle.net/11449/221856 10.24425/jppr.2021.137029 2-s2.0-85108832718 |
url |
http://dx.doi.org/10.24425/jppr.2021.137029 http://hdl.handle.net/11449/221856 |
identifier_str_mv |
Journal of Plant Protection Research, v. 61, n. 2, p. 183-188, 2021. 1899-007X 1427-4345 10.24425/jppr.2021.137029 2-s2.0-85108832718 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Plant Protection Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
183-188 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128247287775232 |