Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2017.08.004 http://hdl.handle.net/11449/163403 |
Resumo: | Objectives: The present study evaluated the trans-dentinal effect of light emitting diodes (LEDs) irradiation on the metabolism of odontoblast-like cells. Methods: Seventy-two dentin discs (0.2 mm thick) were obtained from human molar teeth. MDPC-23 cells (20,000 cells/disc) were seeded on the pulpal side of the discs using DMEM, supplemented with 10% fetal bovine serum (FBS). After 12 h, the culture medium was replaced with DMEM containing 0.5% FBS. After additional 12 h, blue (455 +/- 10 nm) or red (630 +/- 10 nm) LEDs were used at irradiances of 80 and 40 mW/cm(2), respectively, to irradiate the occlusal side of the discs. The energy doses were fixed at 2 or 4 J/cm(2). Cell viability, alkaline phosphatase activity (ALP), total protein production and collagen synthesis were evaluated 72 h after irradiation. Data were submitted to Kruskal-Wallis and Mann-Whitney tests (alpha = 0.05). Results: Red light promoted proliferative effects at the energy dose of 4 J/cm(2) Conversely, cell cultures irradiated with 2 J/cm(2) emitted by the blue light showed reduced viability. ALP production was stimulated by red light in comparison with blue light at 4 J/cm(2). Total protein production was reduced after exposure to blue light at 4 J/cm(2), while no effect was observed on collagen production. Conclusions: Irradiation with red LED at 4 J/cm(2) bio-stimulated the viability of odontoblast-like cells, whilst blue light had unfavorable effects on the cellular metabolism. |
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Repositório Institucional da UNESP |
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Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LEDphototerapylight emitting diodesodontoblastsdentindental pulpObjectives: The present study evaluated the trans-dentinal effect of light emitting diodes (LEDs) irradiation on the metabolism of odontoblast-like cells. Methods: Seventy-two dentin discs (0.2 mm thick) were obtained from human molar teeth. MDPC-23 cells (20,000 cells/disc) were seeded on the pulpal side of the discs using DMEM, supplemented with 10% fetal bovine serum (FBS). After 12 h, the culture medium was replaced with DMEM containing 0.5% FBS. After additional 12 h, blue (455 +/- 10 nm) or red (630 +/- 10 nm) LEDs were used at irradiances of 80 and 40 mW/cm(2), respectively, to irradiate the occlusal side of the discs. The energy doses were fixed at 2 or 4 J/cm(2). Cell viability, alkaline phosphatase activity (ALP), total protein production and collagen synthesis were evaluated 72 h after irradiation. Data were submitted to Kruskal-Wallis and Mann-Whitney tests (alpha = 0.05). Results: Red light promoted proliferative effects at the energy dose of 4 J/cm(2) Conversely, cell cultures irradiated with 2 J/cm(2) emitted by the blue light showed reduced viability. ALP production was stimulated by red light in comparison with blue light at 4 J/cm(2). Total protein production was reduced after exposure to blue light at 4 J/cm(2), while no effect was observed on collagen production. Conclusions: Irradiation with red LED at 4 J/cm(2) bio-stimulated the viability of odontoblast-like cells, whilst blue light had unfavorable effects on the cellular metabolism.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Paraiba, Sch Dent, Cidade Univ S-N, Joao Pessoa, Paraiba, BrazilSao Paulo State Univ, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Rua Humaita 1680, Araraquara, SP, BrazilUniv Fed Uberlandia, Sch Dent, Dept Pediat Dent, Joao Naves de Avila Ave 2121, Uberlandia, MG, BrazilSao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Rua Humaita 1680, Araraquara, SP, BrazilSao Paulo State Univ, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Rua Humaita 1680, Araraquara, SP, BrazilSao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, Rua Humaita 1680, Araraquara, SP, BrazilFAPESP: 2012/17552-2Elsevier B.V.Univ Fed ParaibaUniversidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)Dantas de Almeida, Leopoldina de FatimaBasso, Fernanda Goncalves [UNESP]Silveira Turrioni, Ana Paulade-Souza-Costa, Carlos Alberto [UNESP]Hebling, Josimeri [UNESP]2018-11-26T17:41:53Z2018-11-26T17:41:53Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article258-264application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2017.08.004Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 83, p. 258-264, 2017.0003-9969http://hdl.handle.net/11449/16340310.1016/j.archoralbio.2017.08.004WOS:000413280900038WOS000413280900038.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives Of Oral Biology0,752info:eu-repo/semantics/openAccess2023-10-10T06:03:07Zoai:repositorio.unesp.br:11449/163403Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:30:06.761319Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
title |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
spellingShingle |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED Dantas de Almeida, Leopoldina de Fatima phototerapy light emitting diodes odontoblasts dentin dental pulp |
title_short |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
title_full |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
title_fullStr |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
title_full_unstemmed |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
title_sort |
Metabolism of Odontoblast-like cells submitted to transdentinal irradiation with blue and red LED |
author |
Dantas de Almeida, Leopoldina de Fatima |
author_facet |
Dantas de Almeida, Leopoldina de Fatima Basso, Fernanda Goncalves [UNESP] Silveira Turrioni, Ana Paula de-Souza-Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author_role |
author |
author2 |
Basso, Fernanda Goncalves [UNESP] Silveira Turrioni, Ana Paula de-Souza-Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Paraiba Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Dantas de Almeida, Leopoldina de Fatima Basso, Fernanda Goncalves [UNESP] Silveira Turrioni, Ana Paula de-Souza-Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
dc.subject.por.fl_str_mv |
phototerapy light emitting diodes odontoblasts dentin dental pulp |
topic |
phototerapy light emitting diodes odontoblasts dentin dental pulp |
description |
Objectives: The present study evaluated the trans-dentinal effect of light emitting diodes (LEDs) irradiation on the metabolism of odontoblast-like cells. Methods: Seventy-two dentin discs (0.2 mm thick) were obtained from human molar teeth. MDPC-23 cells (20,000 cells/disc) were seeded on the pulpal side of the discs using DMEM, supplemented with 10% fetal bovine serum (FBS). After 12 h, the culture medium was replaced with DMEM containing 0.5% FBS. After additional 12 h, blue (455 +/- 10 nm) or red (630 +/- 10 nm) LEDs were used at irradiances of 80 and 40 mW/cm(2), respectively, to irradiate the occlusal side of the discs. The energy doses were fixed at 2 or 4 J/cm(2). Cell viability, alkaline phosphatase activity (ALP), total protein production and collagen synthesis were evaluated 72 h after irradiation. Data were submitted to Kruskal-Wallis and Mann-Whitney tests (alpha = 0.05). Results: Red light promoted proliferative effects at the energy dose of 4 J/cm(2) Conversely, cell cultures irradiated with 2 J/cm(2) emitted by the blue light showed reduced viability. ALP production was stimulated by red light in comparison with blue light at 4 J/cm(2). Total protein production was reduced after exposure to blue light at 4 J/cm(2), while no effect was observed on collagen production. Conclusions: Irradiation with red LED at 4 J/cm(2) bio-stimulated the viability of odontoblast-like cells, whilst blue light had unfavorable effects on the cellular metabolism. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-11-26T17:41:53Z 2018-11-26T17:41:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2017.08.004 Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 83, p. 258-264, 2017. 0003-9969 http://hdl.handle.net/11449/163403 10.1016/j.archoralbio.2017.08.004 WOS:000413280900038 WOS000413280900038.pdf |
url |
http://dx.doi.org/10.1016/j.archoralbio.2017.08.004 http://hdl.handle.net/11449/163403 |
identifier_str_mv |
Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 83, p. 258-264, 2017. 0003-9969 10.1016/j.archoralbio.2017.08.004 WOS:000413280900038 WOS000413280900038.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives Of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
258-264 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128369604165632 |