Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2022.103150 http://hdl.handle.net/11449/247812 |
Resumo: | Curcumin has been used as a photosensitizer (PS) for antimicrobial photodynamic chemotherapy (PACT). However, its low solubility, instability, and poor bioavailability challenge its in vivo application. This study aimed to synthesize curcumin-loaded polymeric nanoparticles (curcumin-NP) and determine their antimicrobial and cytotoxic effects. Nanoparticles (NP) were synthesized using polycaprolactone (PCL) as a polymer by the nanoprecipitation method. Curcumin-NP was characterized by particle size, polydispersity index and zeta potential, scanning electron microscopy, and curcumin encapsulation efficiency (EE). Curcumin-NP was compared to free curcumin solubilized in 10% DMSO as photosensitizers for PACT in single and multispecies Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis biofilms. Chlorhexidine 0.12% (CHX) and ultrapure water were used as positive and negative controls. The cytotoxic effect of curcumin-NP was evaluated on human periodontal ligament fibroblast cells (HPLF). Data were analyzed by ANOVA (α=0.05). Curcumin-NP exhibited homogeneity and stability in solution, small particle size, and 67.5% EE of curcumin. Curcumin-NP presented reduced antibiofilm activity at 500 µg/ml, although in planktonic cultures it showed inhibitory and bactericidal effect. Curcumin-NP and curcumin with and without photoactivation were not cytotoxic to HPLF cells. Curcumin-NP has antimicrobial and antibiofilm properties, with better effects when associated with blue light, being a promising therapy for preventing and treating peri-implant diseases. |
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Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilmCurcuminFusobacterium nucleatumNanoparticlePhoto-inactivationPhotodynamic TherapyPorphyromonas gingivalisStreptococcus oralisCurcumin has been used as a photosensitizer (PS) for antimicrobial photodynamic chemotherapy (PACT). However, its low solubility, instability, and poor bioavailability challenge its in vivo application. This study aimed to synthesize curcumin-loaded polymeric nanoparticles (curcumin-NP) and determine their antimicrobial and cytotoxic effects. Nanoparticles (NP) were synthesized using polycaprolactone (PCL) as a polymer by the nanoprecipitation method. Curcumin-NP was characterized by particle size, polydispersity index and zeta potential, scanning electron microscopy, and curcumin encapsulation efficiency (EE). Curcumin-NP was compared to free curcumin solubilized in 10% DMSO as photosensitizers for PACT in single and multispecies Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis biofilms. Chlorhexidine 0.12% (CHX) and ultrapure water were used as positive and negative controls. The cytotoxic effect of curcumin-NP was evaluated on human periodontal ligament fibroblast cells (HPLF). Data were analyzed by ANOVA (α=0.05). Curcumin-NP exhibited homogeneity and stability in solution, small particle size, and 67.5% EE of curcumin. Curcumin-NP presented reduced antibiofilm activity at 500 µg/ml, although in planktonic cultures it showed inhibitory and bactericidal effect. Curcumin-NP and curcumin with and without photoactivation were not cytotoxic to HPLF cells. Curcumin-NP has antimicrobial and antibiofilm properties, with better effects when associated with blue light, being a promising therapy for preventing and treating peri-implant diseases.Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Biomedical Sciences and Comprehensive Care Indiana University School of DentistryDepartment of Drugs and Pharmaceuticals School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), São PauloAmerican Dental Association Science and Research Institute, 211 E. Chicago AveDepartment of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Drugs and Pharmaceuticals School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), São PauloUniversidade Estadual Paulista (UNESP)Indiana University School of DentistryAmerican Dental Association Science and Research InstituteTonon, Caroline Coradi [UNESP]Panariello, BeatrizChorilli, Marlus [UNESP]Spolidorio, Denise Madalena Palomari [UNESP]Duarte, Simone2023-07-29T13:26:30Z2023-07-29T13:26:30Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2022.103150Photodiagnosis and Photodynamic Therapy, v. 40.1873-15971572-1000http://hdl.handle.net/11449/24781210.1016/j.pdpdt.2022.1031502-s2.0-85140880819Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2024-06-24T13:46:32Zoai:repositorio.unesp.br:11449/247812Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:47:33.276687Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
title |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
spellingShingle |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm Tonon, Caroline Coradi [UNESP] Curcumin Fusobacterium nucleatum Nanoparticle Photo-inactivation Photodynamic Therapy Porphyromonas gingivalis Streptococcus oralis |
title_short |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
title_full |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
title_fullStr |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
title_full_unstemmed |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
title_sort |
Effect of curcumin-loaded photoactivatable polymeric nanoparticle on peri-implantitis-related biofilm |
author |
Tonon, Caroline Coradi [UNESP] |
author_facet |
Tonon, Caroline Coradi [UNESP] Panariello, Beatriz Chorilli, Marlus [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Duarte, Simone |
author_role |
author |
author2 |
Panariello, Beatriz Chorilli, Marlus [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Duarte, Simone |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Indiana University School of Dentistry American Dental Association Science and Research Institute |
dc.contributor.author.fl_str_mv |
Tonon, Caroline Coradi [UNESP] Panariello, Beatriz Chorilli, Marlus [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Duarte, Simone |
dc.subject.por.fl_str_mv |
Curcumin Fusobacterium nucleatum Nanoparticle Photo-inactivation Photodynamic Therapy Porphyromonas gingivalis Streptococcus oralis |
topic |
Curcumin Fusobacterium nucleatum Nanoparticle Photo-inactivation Photodynamic Therapy Porphyromonas gingivalis Streptococcus oralis |
description |
Curcumin has been used as a photosensitizer (PS) for antimicrobial photodynamic chemotherapy (PACT). However, its low solubility, instability, and poor bioavailability challenge its in vivo application. This study aimed to synthesize curcumin-loaded polymeric nanoparticles (curcumin-NP) and determine their antimicrobial and cytotoxic effects. Nanoparticles (NP) were synthesized using polycaprolactone (PCL) as a polymer by the nanoprecipitation method. Curcumin-NP was characterized by particle size, polydispersity index and zeta potential, scanning electron microscopy, and curcumin encapsulation efficiency (EE). Curcumin-NP was compared to free curcumin solubilized in 10% DMSO as photosensitizers for PACT in single and multispecies Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis biofilms. Chlorhexidine 0.12% (CHX) and ultrapure water were used as positive and negative controls. The cytotoxic effect of curcumin-NP was evaluated on human periodontal ligament fibroblast cells (HPLF). Data were analyzed by ANOVA (α=0.05). Curcumin-NP exhibited homogeneity and stability in solution, small particle size, and 67.5% EE of curcumin. Curcumin-NP presented reduced antibiofilm activity at 500 µg/ml, although in planktonic cultures it showed inhibitory and bactericidal effect. Curcumin-NP and curcumin with and without photoactivation were not cytotoxic to HPLF cells. Curcumin-NP has antimicrobial and antibiofilm properties, with better effects when associated with blue light, being a promising therapy for preventing and treating peri-implant diseases. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:26:30Z 2023-07-29T13:26:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2022.103150 Photodiagnosis and Photodynamic Therapy, v. 40. 1873-1597 1572-1000 http://hdl.handle.net/11449/247812 10.1016/j.pdpdt.2022.103150 2-s2.0-85140880819 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2022.103150 http://hdl.handle.net/11449/247812 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 40. 1873-1597 1572-1000 10.1016/j.pdpdt.2022.103150 2-s2.0-85140880819 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129462408052736 |