Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens11070749 http://hdl.handle.net/11449/241281 |
Resumo: | Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoG Bartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 105 copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats. |
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Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from PortugalBartonella clarridgeiaeBartonella henselaecat scratch diseasehostsPortugalticksBartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoG Bartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 105 copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para a Ciência e a TecnologiaInstituto de Higiene e Medicina Tropical Universidade Nova de Lisboa (IHMT-UNL), Rua da Junqueira 100Escola de Ciências da Vida Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição 1155, PRGlobal Health and Tropical Medicine Instituto de Higiene e Medicina Tropical Universidade Nova de Lisboa (GHTM-IHMT-UNL), Rua da Junqueira 100Departamento de Patologia Veterinária Universidade Estadual Paulista (FCAV-UNESP), Via de Acesso Paulo Donato Castellane, s/n, SPDepartamento de Patologia Veterinária Universidade Estadual Paulista (FCAV-UNESP), Via de Acesso Paulo Donato Castellane, s/n, SPCNPq: 303701/2021-8Fundação para a Ciência e a Tecnologia: PTDC/CVT-CVT/29073/2017Universidade Nova de Lisboa (IHMT-UNL)Pontifícia Universidade Católica do ParanáUniversidade Nova de Lisboa (GHTM-IHMT-UNL)Universidade Estadual Paulista (UNESP)Torrejón, EstefaniaSanches, Gustavo SeronMoerbeck, LeonardoSantos, LeniraAndré, Marcos Rogério [UNESP]Domingos, AnaAntunes, Sandra2023-03-01T20:54:59Z2023-03-01T20:54:59Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens11070749Pathogens, v. 11, n. 7, 2022.2076-0817http://hdl.handle.net/11449/24128110.3390/pathogens110707492-s2.0-85133521825Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2024-06-07T13:01:32Zoai:repositorio.unesp.br:11449/241281Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:40:31.210951Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
title |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
spellingShingle |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal Torrejón, Estefania Bartonella clarridgeiae Bartonella henselae cat scratch disease hosts Portugal ticks |
title_short |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
title_full |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
title_fullStr |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
title_full_unstemmed |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
title_sort |
Molecular Survey of Bartonella Species in Stray Cats and Dogs, Humans, and Questing Ticks from Portugal |
author |
Torrejón, Estefania |
author_facet |
Torrejón, Estefania Sanches, Gustavo Seron Moerbeck, Leonardo Santos, Lenira André, Marcos Rogério [UNESP] Domingos, Ana Antunes, Sandra |
author_role |
author |
author2 |
Sanches, Gustavo Seron Moerbeck, Leonardo Santos, Lenira André, Marcos Rogério [UNESP] Domingos, Ana Antunes, Sandra |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Nova de Lisboa (IHMT-UNL) Pontifícia Universidade Católica do Paraná Universidade Nova de Lisboa (GHTM-IHMT-UNL) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Torrejón, Estefania Sanches, Gustavo Seron Moerbeck, Leonardo Santos, Lenira André, Marcos Rogério [UNESP] Domingos, Ana Antunes, Sandra |
dc.subject.por.fl_str_mv |
Bartonella clarridgeiae Bartonella henselae cat scratch disease hosts Portugal ticks |
topic |
Bartonella clarridgeiae Bartonella henselae cat scratch disease hosts Portugal ticks |
description |
Bartonella spp. comprises emergent and re-emergent fastidious Gram-negative bacteria with worldwide distribution. Cats are the main reservoir hosts for Bartonella henselae and dogs represent opportunistic hosts for the bacteria. Even though ticks may also play a role in transmission, their competence as vectors for Bartonella spp. has not been totally understood. Considering only a few studies had a focus on screening Bartonella in animals, humans and ectoparasites in Portugal, this study aimed to address the molecular occurrence of Bartonella sp. in 123 stray cats, 25 stray dogs, 30 humans from Lisbon and 236 questing ticks within the country. Using a qPCR targeting the nuoG gene, it was possible to detect Bartonella sp. DNA on 20.32% of cat samples (25/123). From these positive samples, 13 sequences were characterized as B. henselae, 11 as B. clarridgeiae and 1 presented co-infection with both species. The absolute quantification of nuoG Bartonella DNA in sampled cats ranged from 2.78 × 10 to 1.03 × 105 copies/µL. The sampled dogs, humans and ticks were negative. These results showed that B. henselae and B. clarridgeiae are circulating in stray cats from Lisbon. Additional and more extended studies should be conducted to determine the impact of such infections on humans, particularly those in constant and direct contact with cats. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T20:54:59Z 2023-03-01T20:54:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens11070749 Pathogens, v. 11, n. 7, 2022. 2076-0817 http://hdl.handle.net/11449/241281 10.3390/pathogens11070749 2-s2.0-85133521825 |
url |
http://dx.doi.org/10.3390/pathogens11070749 http://hdl.handle.net/11449/241281 |
identifier_str_mv |
Pathogens, v. 11, n. 7, 2022. 2076-0817 10.3390/pathogens11070749 2-s2.0-85133521825 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128398469365760 |