Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascitechnol.v36i2.18372 http://hdl.handle.net/11449/112885 |
Resumo: | Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 10(7) CFU mL(-1) by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30 degrees C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 10(9) CFU mL(-1) during fermentation time. |
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Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginateentrapment cellbatch fermentationBiopolymerZymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 10(7) CFU mL(-1) by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30 degrees C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 10(9) CFU mL(-1) during fermentation time.Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas Sao Jos, Dept Engn & Tecnol Alimentos, BR-15054000 Sao Paulo, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas Sao Jos, Dept Engn & Tecnol Alimentos, BR-15054000 Sao Paulo, BrazilUniv Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacaoUniversidade Estadual Paulista (Unesp)Queiroz Santos, Vidiany Aparecida [UNESP]Del Bianchi, Vanildo Luiz [UNESP]Garcia-Cruz, Crispin Humberto [UNESP]2014-12-03T13:11:08Z2014-12-03T13:11:08Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article349-354application/pdfhttp://dx.doi.org/10.4025/actascitechnol.v36i2.18372Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 36, n. 2, p. 349-354, 2014.1806-2563http://hdl.handle.net/11449/11288510.4025/actascitechnol.v36i2.18372WOS:000334270000022WOS000334270000022.pdf94418940115823503761109299906668Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum: Technology0.2310,168info:eu-repo/semantics/openAccess2023-12-22T06:21:56Zoai:repositorio.unesp.br:11449/112885Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:08.309924Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
spellingShingle |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate Queiroz Santos, Vidiany Aparecida [UNESP] entrapment cell batch fermentation Biopolymer |
title_short |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_full |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_fullStr |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_full_unstemmed |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
title_sort |
Effect of initial pH in levan production by Zymomonas mobilis immobilized in sodium alginate |
author |
Queiroz Santos, Vidiany Aparecida [UNESP] |
author_facet |
Queiroz Santos, Vidiany Aparecida [UNESP] Del Bianchi, Vanildo Luiz [UNESP] Garcia-Cruz, Crispin Humberto [UNESP] |
author_role |
author |
author2 |
Del Bianchi, Vanildo Luiz [UNESP] Garcia-Cruz, Crispin Humberto [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Queiroz Santos, Vidiany Aparecida [UNESP] Del Bianchi, Vanildo Luiz [UNESP] Garcia-Cruz, Crispin Humberto [UNESP] |
dc.subject.por.fl_str_mv |
entrapment cell batch fermentation Biopolymer |
topic |
entrapment cell batch fermentation Biopolymer |
description |
Zymomonas mobilis was immobilized using a cell suspension fixed to 8.6 x 10(7) CFU mL(-1) by spectrophotometry. This biomass was suspended in sodium alginate solution (3%) that was dropped with a hypodermic syringe into 0.2 M calcium chloride solution. Was test two initial pH of fermentation medium (4 and 5) and different sucrose concentrations 15, 20, 25, 30 and 35% at 30 degrees C, without stirring for 24, 48, 72 and 96 hours. The levan production to pH 4 was high in sucrose 25% for 24 (16.51 g L-1) and 48 (15.31 g L-1) hours. The best values obtained to pH 5 was in sucrose 35% during 48 (22.39 g L-1) and 96 (23.5 g L-1) hours, respectively. The maximum levan yield was 40.8% and 22.47% in sucrose 15% to pH 4 and 5, respectively. Substrate consumption to pH 4 was bigger in sucrose 15 (56.4%) and 20% (59.4%) and to pH 5 was in 25 (68.85%) and 35% (64.64%). In relation to immobilization efficiency, Zymomonas mobilis showed high adhesion and colonization in support, indicated by cell growth increased from 107 to 10(9) CFU mL(-1) during fermentation time. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:11:08Z 2014-12-03T13:11:08Z 2014-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascitechnol.v36i2.18372 Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 36, n. 2, p. 349-354, 2014. 1806-2563 http://hdl.handle.net/11449/112885 10.4025/actascitechnol.v36i2.18372 WOS:000334270000022 WOS000334270000022.pdf 9441894011582350 3761109299906668 |
url |
http://dx.doi.org/10.4025/actascitechnol.v36i2.18372 http://hdl.handle.net/11449/112885 |
identifier_str_mv |
Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 36, n. 2, p. 349-354, 2014. 1806-2563 10.4025/actascitechnol.v36i2.18372 WOS:000334270000022 WOS000334270000022.pdf 9441894011582350 3761109299906668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum: Technology 0.231 0,168 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
349-354 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao |
publisher.none.fl_str_mv |
Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129272677662720 |