Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2020.104193 http://hdl.handle.net/11449/199978 |
Resumo: | Salmonella Dublin is a strongly cattle-adapted serovar that has also been responsible for severe invasive infections in humans. Although invasive infections by non-typhoid Salmonella have increased in developed and in developing countries, in sub-Saharan Africa these infections have been frequently related to Salmonella Typhimurium strains from Sequence Type (ST) 313 that harbor a possible virulence marker, the bstA gene, broadly detected in S. Dublin strains. The aims of this study were to verify the frequency of bstA by PCR in 113 Salmonella Dublin strains isolated from humans (83) and animals (30) in Brazil and the expression by RT-PCR of bstA, sopE2 and fliC in six strains isolated from humans (4) and animals (2). Moreover, the invasion capacity in Caco-2 human epithelial cells and U937 human macrophages, plus in vivo virulence analysis in Galleria mellonella and the motility were verified for 20 S. Dublin strains isolated from humans (15) and animals (5). All studied strains presented the bstA gene. The relative expression rates ranged from 0.1 to 2.3 fold change for bstA and from no expression to 16.6 fold change for sopE2, while no expression was detected for fliC. The invasion in Caco-2 cells ranged from 54.0 to 88.9% and in U937 cells from 72.9 to 98.1% in the 20 strains studied. In addition, 17 strains presented a highly virulent profile in the G. mellonella model and 15 strains presented a non-motile profile. In conclusion, the presence and expression of bstA in the S. Dublin strains studied suggested that this gene may influence in the invasive characteristic of this serovar. The low expression of sopE2 in strains from human invasive cases suggested that its expression may not be a limiting factor to the invasion of S. Dublin strains. The absence of fliC expression and the low motility rates observed suggest that the flagella absence may favor the host immune system evasion by S. Dublin and the establishment of infection. Moreover, the high mortality rates observed in vivo in Galleria mellonella reinforce the pathogenic potential of S. Dublin strains. |
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Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in BrazilCell invasionGalleria mellonellaRT-PCRSalmonella DublinST313Salmonella Dublin is a strongly cattle-adapted serovar that has also been responsible for severe invasive infections in humans. Although invasive infections by non-typhoid Salmonella have increased in developed and in developing countries, in sub-Saharan Africa these infections have been frequently related to Salmonella Typhimurium strains from Sequence Type (ST) 313 that harbor a possible virulence marker, the bstA gene, broadly detected in S. Dublin strains. The aims of this study were to verify the frequency of bstA by PCR in 113 Salmonella Dublin strains isolated from humans (83) and animals (30) in Brazil and the expression by RT-PCR of bstA, sopE2 and fliC in six strains isolated from humans (4) and animals (2). Moreover, the invasion capacity in Caco-2 human epithelial cells and U937 human macrophages, plus in vivo virulence analysis in Galleria mellonella and the motility were verified for 20 S. Dublin strains isolated from humans (15) and animals (5). All studied strains presented the bstA gene. The relative expression rates ranged from 0.1 to 2.3 fold change for bstA and from no expression to 16.6 fold change for sopE2, while no expression was detected for fliC. The invasion in Caco-2 cells ranged from 54.0 to 88.9% and in U937 cells from 72.9 to 98.1% in the 20 strains studied. In addition, 17 strains presented a highly virulent profile in the G. mellonella model and 15 strains presented a non-motile profile. In conclusion, the presence and expression of bstA in the S. Dublin strains studied suggested that this gene may influence in the invasive characteristic of this serovar. The low expression of sopE2 in strains from human invasive cases suggested that its expression may not be a limiting factor to the invasion of S. Dublin strains. The absence of fliC expression and the low motility rates observed suggest that the flagella absence may favor the host immune system evasion by S. Dublin and the establishment of infection. Moreover, the high mortality rates observed in vivo in Galleria mellonella reinforce the pathogenic potential of S. Dublin strains.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP Departamento de Análises Clínicas Toxicológicas e Bromatológicas, Av. do Café, s/nFaculdade de Ciências Farmacêuticas - Câmpus de Araraquara - UNESP Departamento de Ciências Biológicas Rodovia Araraquara Jaú, Km 01, s/nFundação Oswaldo Cruz - FIOCRUZ, Av. Brasil, 4365, Pavilhão Rocha LimaInstituto Adolfo Lutz Centro de Bacteriologia, Av. Dr. Arnaldo, 351Faculdade de Ciências Farmacêuticas - Câmpus de Araraquara - UNESP Departamento de Ciências Biológicas Rodovia Araraquara Jaú, Km 01, s/nFAPESP: 2013/25191-3FAPESP: 2016/24716-3FAPESP: 2017/05756-7FAPESP: 2019/06947-6CNPq: CNPq 303475/2015-3CNPq: CNPq 304399/2018-3Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Fundação Oswaldo Cruz - FIOCRUZCentro de BacteriologiaVilela, Felipe PinheiroGomes, Carolina NogueiraPaziani, Mário HenriqueBraz, Vânia Santos [UNESP]Rodrigues, Dália dos PrazeresCosta, Renata GarciaTiba-Casas, Monique RibeiroKress, Márcia Regina von ZeskaFalcão, Juliana PfrimerCampioni, Fábio2020-12-12T01:54:19Z2020-12-12T01:54:19Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.meegid.2020.104193Infection, Genetics and Evolution, v. 80.1567-72571567-1348http://hdl.handle.net/11449/19997810.1016/j.meegid.2020.1041932-s2.0-85078250629Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection, Genetics and Evolutioninfo:eu-repo/semantics/openAccess2024-06-24T13:07:13Zoai:repositorio.unesp.br:11449/199978Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:14:38.541457Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
title |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
spellingShingle |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil Vilela, Felipe Pinheiro Cell invasion Galleria mellonella RT-PCR Salmonella Dublin ST313 |
title_short |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
title_full |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
title_fullStr |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
title_full_unstemmed |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
title_sort |
Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil |
author |
Vilela, Felipe Pinheiro |
author_facet |
Vilela, Felipe Pinheiro Gomes, Carolina Nogueira Paziani, Mário Henrique Braz, Vânia Santos [UNESP] Rodrigues, Dália dos Prazeres Costa, Renata Garcia Tiba-Casas, Monique Ribeiro Kress, Márcia Regina von Zeska Falcão, Juliana Pfrimer Campioni, Fábio |
author_role |
author |
author2 |
Gomes, Carolina Nogueira Paziani, Mário Henrique Braz, Vânia Santos [UNESP] Rodrigues, Dália dos Prazeres Costa, Renata Garcia Tiba-Casas, Monique Ribeiro Kress, Márcia Regina von Zeska Falcão, Juliana Pfrimer Campioni, Fábio |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Fundação Oswaldo Cruz - FIOCRUZ Centro de Bacteriologia |
dc.contributor.author.fl_str_mv |
Vilela, Felipe Pinheiro Gomes, Carolina Nogueira Paziani, Mário Henrique Braz, Vânia Santos [UNESP] Rodrigues, Dália dos Prazeres Costa, Renata Garcia Tiba-Casas, Monique Ribeiro Kress, Márcia Regina von Zeska Falcão, Juliana Pfrimer Campioni, Fábio |
dc.subject.por.fl_str_mv |
Cell invasion Galleria mellonella RT-PCR Salmonella Dublin ST313 |
topic |
Cell invasion Galleria mellonella RT-PCR Salmonella Dublin ST313 |
description |
Salmonella Dublin is a strongly cattle-adapted serovar that has also been responsible for severe invasive infections in humans. Although invasive infections by non-typhoid Salmonella have increased in developed and in developing countries, in sub-Saharan Africa these infections have been frequently related to Salmonella Typhimurium strains from Sequence Type (ST) 313 that harbor a possible virulence marker, the bstA gene, broadly detected in S. Dublin strains. The aims of this study were to verify the frequency of bstA by PCR in 113 Salmonella Dublin strains isolated from humans (83) and animals (30) in Brazil and the expression by RT-PCR of bstA, sopE2 and fliC in six strains isolated from humans (4) and animals (2). Moreover, the invasion capacity in Caco-2 human epithelial cells and U937 human macrophages, plus in vivo virulence analysis in Galleria mellonella and the motility were verified for 20 S. Dublin strains isolated from humans (15) and animals (5). All studied strains presented the bstA gene. The relative expression rates ranged from 0.1 to 2.3 fold change for bstA and from no expression to 16.6 fold change for sopE2, while no expression was detected for fliC. The invasion in Caco-2 cells ranged from 54.0 to 88.9% and in U937 cells from 72.9 to 98.1% in the 20 strains studied. In addition, 17 strains presented a highly virulent profile in the G. mellonella model and 15 strains presented a non-motile profile. In conclusion, the presence and expression of bstA in the S. Dublin strains studied suggested that this gene may influence in the invasive characteristic of this serovar. The low expression of sopE2 in strains from human invasive cases suggested that its expression may not be a limiting factor to the invasion of S. Dublin strains. The absence of fliC expression and the low motility rates observed suggest that the flagella absence may favor the host immune system evasion by S. Dublin and the establishment of infection. Moreover, the high mortality rates observed in vivo in Galleria mellonella reinforce the pathogenic potential of S. Dublin strains. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:54:19Z 2020-12-12T01:54:19Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2020.104193 Infection, Genetics and Evolution, v. 80. 1567-7257 1567-1348 http://hdl.handle.net/11449/199978 10.1016/j.meegid.2020.104193 2-s2.0-85078250629 |
url |
http://dx.doi.org/10.1016/j.meegid.2020.104193 http://hdl.handle.net/11449/199978 |
identifier_str_mv |
Infection, Genetics and Evolution, v. 80. 1567-7257 1567-1348 10.1016/j.meegid.2020.104193 2-s2.0-85078250629 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection, Genetics and Evolution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128486483689472 |