Function of cryopreserved cat ovarian tissue after autotransplantation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ani9121065 http://hdl.handle.net/11449/199773 |
Resumo: | The aim of this study was to assess a slow-freezing protocol of cat ovarian tissue cryopreservation using autotransplantation. Four adult queens were ovariohysterectomized and the ovaries were fragmented and cryopreserved. After one week, the grafts were thawed and autografted to the subcutaneous tissue of the dorsal neck of each queen, then randomly removed after 7, 14, 28, 49, and 63 days after transplantation. Percentages of morphologically normal primordial and growing follicles (MNFs) were 88% and 97%, respectively, in fresh tissue samples (fresh controls), and 74% and 100%, respectively, immediately after thawing (cryo D0). No MNFs were found after 49 days of transplantation. In both fresh control and cryo D0 fragments, granulosa cells were frequently in proliferation. Two morphologically normal antral follicles were detected in one queen on Day 28 post-transplantation. Connective tissue fibers increased, suggesting replacement of active ovarian cortex by fibrous tissue. Tissue vascularization was observed at 7 days after grafting, and wide blood vessels were clearly visible on Days 49 and 63. In conclusion, although follicular survival was low after cryopreservation and grafting of cat ovarian tissue, follicles were able to develop up to the antral stage, which is an encouraging outcome. |
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Function of cryopreserved cat ovarian tissue after autotransplantationAntral follicleCryopreservationFelidsOvaryTransplantThe aim of this study was to assess a slow-freezing protocol of cat ovarian tissue cryopreservation using autotransplantation. Four adult queens were ovariohysterectomized and the ovaries were fragmented and cryopreserved. After one week, the grafts were thawed and autografted to the subcutaneous tissue of the dorsal neck of each queen, then randomly removed after 7, 14, 28, 49, and 63 days after transplantation. Percentages of morphologically normal primordial and growing follicles (MNFs) were 88% and 97%, respectively, in fresh tissue samples (fresh controls), and 74% and 100%, respectively, immediately after thawing (cryo D0). No MNFs were found after 49 days of transplantation. In both fresh control and cryo D0 fragments, granulosa cells were frequently in proliferation. Two morphologically normal antral follicles were detected in one queen on Day 28 post-transplantation. Connective tissue fibers increased, suggesting replacement of active ovarian cortex by fibrous tissue. Tissue vascularization was observed at 7 days after grafting, and wide blood vessels were clearly visible on Days 49 and 63. In conclusion, although follicular survival was low after cryopreservation and grafting of cat ovarian tissue, follicles were able to develop up to the antral stage, which is an encouraging outcome.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Wallonie-Bruxelles InternationalDepartamento de Ciências Fisiológicas Instituto de Ciências Biológicas Campus Universitário Darcy Ribeiro Universidade de Brasília, Distrito FederalInstitut de Recherche Expérimentale et Clinique Pôle de Recherche en Gynécologie Université Catholique de Louvain, Avenue Mounier, 52 bte B1.52.02Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265Instituto Federal de Educação Ciência e Tecnologia do Amazonas Campus Manaus Zona Leste-IFAM/CMZL, Avenida Cosme Ferreira, 8045Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265CAPES: #013/14Wallonie-Bruxelles International: #013/14CAPES: 001Universidade de Brasília (UnB)Université Catholique de LouvainUniversidade Estadual Paulista (Unesp)Ciência e Tecnologia do AmazonasVilela, Janice M. V.Leonel, Ellen C. R. [UNESP]Gonçalves, Liudimila P.Paiva, Raísa E. G.Amaral, Rodrigo S.Amorim, Christiani A.Lucci, Carolina M.2020-12-12T01:48:54Z2020-12-12T01:48:54Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani9121065Animals, v. 9, n. 12, 2019.2076-2615http://hdl.handle.net/11449/19977310.3390/ani91210652-s2.0-85076113242Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2021-10-23T09:41:37Zoai:repositorio.unesp.br:11449/199773Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:52:07.148973Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Function of cryopreserved cat ovarian tissue after autotransplantation |
title |
Function of cryopreserved cat ovarian tissue after autotransplantation |
spellingShingle |
Function of cryopreserved cat ovarian tissue after autotransplantation Vilela, Janice M. V. Antral follicle Cryopreservation Felids Ovary Transplant |
title_short |
Function of cryopreserved cat ovarian tissue after autotransplantation |
title_full |
Function of cryopreserved cat ovarian tissue after autotransplantation |
title_fullStr |
Function of cryopreserved cat ovarian tissue after autotransplantation |
title_full_unstemmed |
Function of cryopreserved cat ovarian tissue after autotransplantation |
title_sort |
Function of cryopreserved cat ovarian tissue after autotransplantation |
author |
Vilela, Janice M. V. |
author_facet |
Vilela, Janice M. V. Leonel, Ellen C. R. [UNESP] Gonçalves, Liudimila P. Paiva, Raísa E. G. Amaral, Rodrigo S. Amorim, Christiani A. Lucci, Carolina M. |
author_role |
author |
author2 |
Leonel, Ellen C. R. [UNESP] Gonçalves, Liudimila P. Paiva, Raísa E. G. Amaral, Rodrigo S. Amorim, Christiani A. Lucci, Carolina M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Brasília (UnB) Université Catholique de Louvain Universidade Estadual Paulista (Unesp) Ciência e Tecnologia do Amazonas |
dc.contributor.author.fl_str_mv |
Vilela, Janice M. V. Leonel, Ellen C. R. [UNESP] Gonçalves, Liudimila P. Paiva, Raísa E. G. Amaral, Rodrigo S. Amorim, Christiani A. Lucci, Carolina M. |
dc.subject.por.fl_str_mv |
Antral follicle Cryopreservation Felids Ovary Transplant |
topic |
Antral follicle Cryopreservation Felids Ovary Transplant |
description |
The aim of this study was to assess a slow-freezing protocol of cat ovarian tissue cryopreservation using autotransplantation. Four adult queens were ovariohysterectomized and the ovaries were fragmented and cryopreserved. After one week, the grafts were thawed and autografted to the subcutaneous tissue of the dorsal neck of each queen, then randomly removed after 7, 14, 28, 49, and 63 days after transplantation. Percentages of morphologically normal primordial and growing follicles (MNFs) were 88% and 97%, respectively, in fresh tissue samples (fresh controls), and 74% and 100%, respectively, immediately after thawing (cryo D0). No MNFs were found after 49 days of transplantation. In both fresh control and cryo D0 fragments, granulosa cells were frequently in proliferation. Two morphologically normal antral follicles were detected in one queen on Day 28 post-transplantation. Connective tissue fibers increased, suggesting replacement of active ovarian cortex by fibrous tissue. Tissue vascularization was observed at 7 days after grafting, and wide blood vessels were clearly visible on Days 49 and 63. In conclusion, although follicular survival was low after cryopreservation and grafting of cat ovarian tissue, follicles were able to develop up to the antral stage, which is an encouraging outcome. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-12T01:48:54Z 2020-12-12T01:48:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ani9121065 Animals, v. 9, n. 12, 2019. 2076-2615 http://hdl.handle.net/11449/199773 10.3390/ani9121065 2-s2.0-85076113242 |
url |
http://dx.doi.org/10.3390/ani9121065 http://hdl.handle.net/11449/199773 |
identifier_str_mv |
Animals, v. 9, n. 12, 2019. 2076-2615 10.3390/ani9121065 2-s2.0-85076113242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128575947145216 |