llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.exger.2017.07.006 http://hdl.handle.net/11449/177035 |
Resumo: | During perimenopause, oxidative stress increases, which may result in disruption of bone turnover, and consequently in osteoporosis. The use of antioxidants may be an effective nutritional approach to reducing osteoporosis in this period of life. Mate tea (MT) (Ilex paraguariensis), a typical and inexpensive beverage consumed in the Brazilian south-east, Argentina and Uruguay, increases antioxidant defense. Our hypothesis was that MT would decrease oxidative stress and mitigate bone deterioration. To test this, we analyzed oxidative stress markers of bone turnover, and local and systemic markers of bone metabolism of rats during natural perimenopause. Female Wistar rats (aged 16 months) in proven perimenopause period received 20 mg/kg BW/day of mate tea, by gavage (PM + MT Group, n = 10) or water (PM Group, n = 10). Female rats aged 4 months (AD Group, n = 10) received water. The treatment period was four weeks. MT minimized the deterioration of rat microarchitecture, characterized by increase in the bone trabecular area, number of osteocytes and areal bone mineral density. These results were accompanied by a lower level of malondialdehyde, an oxidative stress marker, in femoral tissue homogenate. Plasmatic tartrate-resistant acid phosphatase, a typical osteoclastic function marker, decreases after treatment, indicating a decrease in osteoclastic function. MT also modified the immunostaining pattern of bone metabolism markers, decreasing the receptor activator of nuclear factor kappa-B ligant (RANKL), superoxide dismutase isoform 2 (SOD2) and increasing osteoprotegerin (OPG), a decoy receptor for the RANKL, which positively modulates bone mass. These results suggested MT was capable of decreasing bone resorption by inhibiting the osteoclastogenesis in a RANKL-dependent signaling pathway activated by oxidative stress. Taken together, the results indicated that MT minimized bone loss in perimenopause and this effect is at least partly due to the decrease in oxidative stress, confirming our hypothesis. |
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llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopauseBoneIlex paraguariensisOxidative stressPerimenopauseDuring perimenopause, oxidative stress increases, which may result in disruption of bone turnover, and consequently in osteoporosis. The use of antioxidants may be an effective nutritional approach to reducing osteoporosis in this period of life. Mate tea (MT) (Ilex paraguariensis), a typical and inexpensive beverage consumed in the Brazilian south-east, Argentina and Uruguay, increases antioxidant defense. Our hypothesis was that MT would decrease oxidative stress and mitigate bone deterioration. To test this, we analyzed oxidative stress markers of bone turnover, and local and systemic markers of bone metabolism of rats during natural perimenopause. Female Wistar rats (aged 16 months) in proven perimenopause period received 20 mg/kg BW/day of mate tea, by gavage (PM + MT Group, n = 10) or water (PM Group, n = 10). Female rats aged 4 months (AD Group, n = 10) received water. The treatment period was four weeks. MT minimized the deterioration of rat microarchitecture, characterized by increase in the bone trabecular area, number of osteocytes and areal bone mineral density. These results were accompanied by a lower level of malondialdehyde, an oxidative stress marker, in femoral tissue homogenate. Plasmatic tartrate-resistant acid phosphatase, a typical osteoclastic function marker, decreases after treatment, indicating a decrease in osteoclastic function. MT also modified the immunostaining pattern of bone metabolism markers, decreasing the receptor activator of nuclear factor kappa-B ligant (RANKL), superoxide dismutase isoform 2 (SOD2) and increasing osteoprotegerin (OPG), a decoy receptor for the RANKL, which positively modulates bone mass. These results suggested MT was capable of decreasing bone resorption by inhibiting the osteoclastogenesis in a RANKL-dependent signaling pathway activated by oxidative stress. Taken together, the results indicated that MT minimized bone loss in perimenopause and this effect is at least partly due to the decrease in oxidative stress, confirming our hypothesis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas-SBFis São Paulo State University (Unesp) School of DentistryDepartment of Pathology and Clinical Propaedeutics São Paulo State University (Unesp) School of DentistryDepartment of Clinical Toxicological and Bromatological Analysis Faculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (USP)Department of Support Animal Production and Health São Paulo State University (Unesp) Veterinary Medicine SchoolDepartment of Physiology Health Basic Sciences Institute Federal University of Rio Grande do Sul (UFRGS)Department of Analytical Chemistry and Physical Chemistry -School of Pharmacy and Biochemistry University of Buenos AiresDepartment of Basic Sciences São Paulo State University (Unesp) School of DentistryPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicas-SBFis São Paulo State University (Unesp) School of DentistryDepartment of Pathology and Clinical Propaedeutics São Paulo State University (Unesp) School of DentistryDepartment of Support Animal Production and Health São Paulo State University (Unesp) Veterinary Medicine SchoolDepartment of Basic Sciences São Paulo State University (Unesp) School of DentistryUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Federal University of Rio Grande do Sul (UFRGS)University of Buenos AiresPereira, Camila Scacco [UNESP]Stringhetta-Garcia, Camila Tami [UNESP]da Silva Xavier, Lilian [UNESP]Tirapeli, Keny Gonçalves [UNESP]Pereira, Ariana Aparecida Ferreira [UNESP]Kayahara, GiselIi Mitsuy [UNESP]Tramarim, José Marcelo [UNESP]Crivelini, Marcelo Macedo [UNESP]Padovani, Karina StringhettaLeopoldino, Andréia MachadoLouzada, Mário Jefferson Quirino [UNESP]Belló-Klein, AdrianeLlesuy, Susana FranciscaErvolino, Edilson [UNESP]Dornelles, Rita Cássia Menegati [UNESP]Chaves-Neto, Antonio Hernandes [UNESP]Nakamune, Ana Cláudia de Melo Stevanato [UNESP]2018-12-11T17:23:34Z2018-12-11T17:23:34Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-152application/pdfhttp://dx.doi.org/10.1016/j.exger.2017.07.006Experimental Gerontology, v. 98, p. 148-152.1873-68150531-5565http://hdl.handle.net/11449/17703510.1016/j.exger.2017.07.0062-s2.0-850280700462-s2.0-85028070046.pdf4408095517346846954425748251267154359024227848890000-0003-4859-05830000-0003-0783-6612Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Gerontology1,450info:eu-repo/semantics/openAccess2024-06-24T14:52:02Zoai:repositorio.unesp.br:11449/177035Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:47:02.433687Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
title |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
spellingShingle |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause Pereira, Camila Scacco [UNESP] Bone Ilex paraguariensis Oxidative stress Perimenopause |
title_short |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
title_full |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
title_fullStr |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
title_full_unstemmed |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
title_sort |
llex paraguariensis decreases oxidative stress in bone and mitigates the damage in rats during perimenopause |
author |
Pereira, Camila Scacco [UNESP] |
author_facet |
Pereira, Camila Scacco [UNESP] Stringhetta-Garcia, Camila Tami [UNESP] da Silva Xavier, Lilian [UNESP] Tirapeli, Keny Gonçalves [UNESP] Pereira, Ariana Aparecida Ferreira [UNESP] Kayahara, GiselIi Mitsuy [UNESP] Tramarim, José Marcelo [UNESP] Crivelini, Marcelo Macedo [UNESP] Padovani, Karina Stringhetta Leopoldino, Andréia Machado Louzada, Mário Jefferson Quirino [UNESP] Belló-Klein, Adriane Llesuy, Susana Francisca Ervolino, Edilson [UNESP] Dornelles, Rita Cássia Menegati [UNESP] Chaves-Neto, Antonio Hernandes [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] |
author_role |
author |
author2 |
Stringhetta-Garcia, Camila Tami [UNESP] da Silva Xavier, Lilian [UNESP] Tirapeli, Keny Gonçalves [UNESP] Pereira, Ariana Aparecida Ferreira [UNESP] Kayahara, GiselIi Mitsuy [UNESP] Tramarim, José Marcelo [UNESP] Crivelini, Marcelo Macedo [UNESP] Padovani, Karina Stringhetta Leopoldino, Andréia Machado Louzada, Mário Jefferson Quirino [UNESP] Belló-Klein, Adriane Llesuy, Susana Francisca Ervolino, Edilson [UNESP] Dornelles, Rita Cássia Menegati [UNESP] Chaves-Neto, Antonio Hernandes [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Federal University of Rio Grande do Sul (UFRGS) University of Buenos Aires |
dc.contributor.author.fl_str_mv |
Pereira, Camila Scacco [UNESP] Stringhetta-Garcia, Camila Tami [UNESP] da Silva Xavier, Lilian [UNESP] Tirapeli, Keny Gonçalves [UNESP] Pereira, Ariana Aparecida Ferreira [UNESP] Kayahara, GiselIi Mitsuy [UNESP] Tramarim, José Marcelo [UNESP] Crivelini, Marcelo Macedo [UNESP] Padovani, Karina Stringhetta Leopoldino, Andréia Machado Louzada, Mário Jefferson Quirino [UNESP] Belló-Klein, Adriane Llesuy, Susana Francisca Ervolino, Edilson [UNESP] Dornelles, Rita Cássia Menegati [UNESP] Chaves-Neto, Antonio Hernandes [UNESP] Nakamune, Ana Cláudia de Melo Stevanato [UNESP] |
dc.subject.por.fl_str_mv |
Bone Ilex paraguariensis Oxidative stress Perimenopause |
topic |
Bone Ilex paraguariensis Oxidative stress Perimenopause |
description |
During perimenopause, oxidative stress increases, which may result in disruption of bone turnover, and consequently in osteoporosis. The use of antioxidants may be an effective nutritional approach to reducing osteoporosis in this period of life. Mate tea (MT) (Ilex paraguariensis), a typical and inexpensive beverage consumed in the Brazilian south-east, Argentina and Uruguay, increases antioxidant defense. Our hypothesis was that MT would decrease oxidative stress and mitigate bone deterioration. To test this, we analyzed oxidative stress markers of bone turnover, and local and systemic markers of bone metabolism of rats during natural perimenopause. Female Wistar rats (aged 16 months) in proven perimenopause period received 20 mg/kg BW/day of mate tea, by gavage (PM + MT Group, n = 10) or water (PM Group, n = 10). Female rats aged 4 months (AD Group, n = 10) received water. The treatment period was four weeks. MT minimized the deterioration of rat microarchitecture, characterized by increase in the bone trabecular area, number of osteocytes and areal bone mineral density. These results were accompanied by a lower level of malondialdehyde, an oxidative stress marker, in femoral tissue homogenate. Plasmatic tartrate-resistant acid phosphatase, a typical osteoclastic function marker, decreases after treatment, indicating a decrease in osteoclastic function. MT also modified the immunostaining pattern of bone metabolism markers, decreasing the receptor activator of nuclear factor kappa-B ligant (RANKL), superoxide dismutase isoform 2 (SOD2) and increasing osteoprotegerin (OPG), a decoy receptor for the RANKL, which positively modulates bone mass. These results suggested MT was capable of decreasing bone resorption by inhibiting the osteoclastogenesis in a RANKL-dependent signaling pathway activated by oxidative stress. Taken together, the results indicated that MT minimized bone loss in perimenopause and this effect is at least partly due to the decrease in oxidative stress, confirming our hypothesis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-12-11T17:23:34Z 2018-12-11T17:23:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.exger.2017.07.006 Experimental Gerontology, v. 98, p. 148-152. 1873-6815 0531-5565 http://hdl.handle.net/11449/177035 10.1016/j.exger.2017.07.006 2-s2.0-85028070046 2-s2.0-85028070046.pdf 4408095517346846 9544257482512671 5435902422784889 0000-0003-4859-0583 0000-0003-0783-6612 |
url |
http://dx.doi.org/10.1016/j.exger.2017.07.006 http://hdl.handle.net/11449/177035 |
identifier_str_mv |
Experimental Gerontology, v. 98, p. 148-152. 1873-6815 0531-5565 10.1016/j.exger.2017.07.006 2-s2.0-85028070046 2-s2.0-85028070046.pdf 4408095517346846 9544257482512671 5435902422784889 0000-0003-4859-0583 0000-0003-0783-6612 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental Gerontology 1,450 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
148-152 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129357795819520 |