Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1519-6984.253436 http://hdl.handle.net/11449/218119 |
Resumo: | The in vitro sporulation of Didymella bryoniae is of great importance for studies that require pure inoculum and in large quantities. Thus, the objectives of this study were to identify the best condition for D. bryoniae sporulation combining different light spectra (UV-A or UV-B light, white light, and continuous dark), with distinct culture media (PDA, V8, ML, and PDAB) and, to evaluate fungus’ survivability stored at -20°C over time. The fungus samples were only able to sporulate when subjected to the UV-B light treatment, regardless of the culture medium. The highest appearance of spores conidium type was observed in the PDAB medium, and the lowest production occurred in the ML medium. Reproductive structures, such as perithecia and pycnidia, were observed in all culture media. However, there was considerable variation in the amount of each structure between the different culture media. The ML and V8 media showed a greater number of perithecia and the PDA and PDAB media presented a greater proportion of pycnidia compared to perithecia. The storage duration at -20°C did not affect mycelial growth or mycelial growth rate. In conclusion, the UV-B light is essential for D. bryoniae in vitro sporulation. Moreover, the culture medium composition influences the type of fungal structure produced, as well as spores’ size and quantity. Freezing at -20°C is an efficient technique that can be used to store D. bryoniae for at least five months without loss of viability. |
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Repositório Institucional da UNESP |
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Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperatureFornecendo inóculo para estudos de Didymella bryoniae: o efeito do espectro de luz e armazenamento em baixa temperaturafungi preservationgummy stem blightsporulationUV-B lightPreservação de fungoscrestamento gomoso do cauleesporulaçãoLuz UV-BThe in vitro sporulation of Didymella bryoniae is of great importance for studies that require pure inoculum and in large quantities. Thus, the objectives of this study were to identify the best condition for D. bryoniae sporulation combining different light spectra (UV-A or UV-B light, white light, and continuous dark), with distinct culture media (PDA, V8, ML, and PDAB) and, to evaluate fungus’ survivability stored at -20°C over time. The fungus samples were only able to sporulate when subjected to the UV-B light treatment, regardless of the culture medium. The highest appearance of spores conidium type was observed in the PDAB medium, and the lowest production occurred in the ML medium. Reproductive structures, such as perithecia and pycnidia, were observed in all culture media. However, there was considerable variation in the amount of each structure between the different culture media. The ML and V8 media showed a greater number of perithecia and the PDA and PDAB media presented a greater proportion of pycnidia compared to perithecia. The storage duration at -20°C did not affect mycelial growth or mycelial growth rate. In conclusion, the UV-B light is essential for D. bryoniae in vitro sporulation. Moreover, the culture medium composition influences the type of fungal structure produced, as well as spores’ size and quantity. Freezing at -20°C is an efficient technique that can be used to store D. bryoniae for at least five months without loss of viability.A esporulação de Didymella bryoniae in vitro é de grande importância para estudos que requerem inóculo puro e em grandes quantidades. Assim, os objetivos deste estudo foram identificar a melhor condição para esporulação de D. bryoniae combinando diferentes espectros de luz (luz UV-A ou UV-B, luz branca e escuro contínuo) com distintos meios de cultura (PDA, V8, ML e PDAB) e, avaliar a sobrevivência do fungo armazenado a -20°C ao longo do tempo. As amostras de fungo só esporularam quando submetidas ao tratamento com luz UV-B, independentemente do meio de cultura. Maior aparecimento de esporos do tipo conídio foi observado no meio PDAB, e a menor produção ocorreu no meio ML. Estruturas reprodutivas, como peritécios e picnídeos, foram observadas em todos os meios de cultura. No entanto, houve uma variação considerável na quantidade de cada estrutura entre os diferentes meios de cultura. Os meios ML e V8 apresentaram maior número de peritécios e os meios PDA e PDAB apresentaram maior proporção de picnídeos em relação aos peritécios. A duração do armazenamento a -20°C não afetou o crescimento micelial ou a taxa de crescimento micelial. Em conclusão, a luz UV-B é essencial para a esporulação de D. bryoniae in vitro. Além disso, a composição do meio de cultura influencia o tipo de estrutura fúngica produzida, bem como o tamanho e a quantidade dos esporos. O congelamento a -20°C é uma técnica eficiente que pode ser usada para armazenar D. bryoniae por pelo menos cinco meses sem perda de viabilidadeCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasInstituto Taquaritinguense de Ensino Superior “Dr. Aristides de Carvalho Schlobach” – ITESUniversity of Alberta, Department of AgriculturalUniversidade Estadual Paulista, Faculdade de Ciências Agrárias e VeterináriasCAPES: 001Instituto Internacional de EcologiaUniversidade Estadual Paulista (UNESP)Instituto Taquaritinguense de Ensino Superior “Dr. Aristides de Carvalho Schlobach” – ITESUniversity of AlbertaVirtuoso, M. C. S. [UNESP]Silva, E. H. C.Silva, E. M. [UNESP]Valente, T. S. [UNESP]Vargas, P. F. [UNESP]Braz, L. T. [UNESP]Panizzi, R. C. [UNESP]2022-04-28T16:55:57Z2022-04-28T16:55:57Z2021-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1519-6984.253436Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2021.1519-69841678-4375http://hdl.handle.net/11449/21811910.1590/1519-6984.253436S1519-69842024000100158S1519-69842024000100158.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2023-11-19T06:15:33Zoai:repositorio.unesp.br:11449/218119Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:10:14.349999Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature Fornecendo inóculo para estudos de Didymella bryoniae: o efeito do espectro de luz e armazenamento em baixa temperatura |
title |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature |
spellingShingle |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature Virtuoso, M. C. S. [UNESP] fungi preservation gummy stem blight sporulation UV-B light Preservação de fungos crestamento gomoso do caule esporulação Luz UV-B |
title_short |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature |
title_full |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature |
title_fullStr |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature |
title_full_unstemmed |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature |
title_sort |
Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature |
author |
Virtuoso, M. C. S. [UNESP] |
author_facet |
Virtuoso, M. C. S. [UNESP] Silva, E. H. C. Silva, E. M. [UNESP] Valente, T. S. [UNESP] Vargas, P. F. [UNESP] Braz, L. T. [UNESP] Panizzi, R. C. [UNESP] |
author_role |
author |
author2 |
Silva, E. H. C. Silva, E. M. [UNESP] Valente, T. S. [UNESP] Vargas, P. F. [UNESP] Braz, L. T. [UNESP] Panizzi, R. C. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto Taquaritinguense de Ensino Superior “Dr. Aristides de Carvalho Schlobach” – ITES University of Alberta |
dc.contributor.author.fl_str_mv |
Virtuoso, M. C. S. [UNESP] Silva, E. H. C. Silva, E. M. [UNESP] Valente, T. S. [UNESP] Vargas, P. F. [UNESP] Braz, L. T. [UNESP] Panizzi, R. C. [UNESP] |
dc.subject.por.fl_str_mv |
fungi preservation gummy stem blight sporulation UV-B light Preservação de fungos crestamento gomoso do caule esporulação Luz UV-B |
topic |
fungi preservation gummy stem blight sporulation UV-B light Preservação de fungos crestamento gomoso do caule esporulação Luz UV-B |
description |
The in vitro sporulation of Didymella bryoniae is of great importance for studies that require pure inoculum and in large quantities. Thus, the objectives of this study were to identify the best condition for D. bryoniae sporulation combining different light spectra (UV-A or UV-B light, white light, and continuous dark), with distinct culture media (PDA, V8, ML, and PDAB) and, to evaluate fungus’ survivability stored at -20°C over time. The fungus samples were only able to sporulate when subjected to the UV-B light treatment, regardless of the culture medium. The highest appearance of spores conidium type was observed in the PDAB medium, and the lowest production occurred in the ML medium. Reproductive structures, such as perithecia and pycnidia, were observed in all culture media. However, there was considerable variation in the amount of each structure between the different culture media. The ML and V8 media showed a greater number of perithecia and the PDA and PDAB media presented a greater proportion of pycnidia compared to perithecia. The storage duration at -20°C did not affect mycelial growth or mycelial growth rate. In conclusion, the UV-B light is essential for D. bryoniae in vitro sporulation. Moreover, the culture medium composition influences the type of fungal structure produced, as well as spores’ size and quantity. Freezing at -20°C is an efficient technique that can be used to store D. bryoniae for at least five months without loss of viability. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-20 2022-04-28T16:55:57Z 2022-04-28T16:55:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1519-6984.253436 Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2021. 1519-6984 1678-4375 http://hdl.handle.net/11449/218119 10.1590/1519-6984.253436 S1519-69842024000100158 S1519-69842024000100158.pdf |
url |
http://dx.doi.org/10.1590/1519-6984.253436 http://hdl.handle.net/11449/218119 |
identifier_str_mv |
Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 84, p. -, 2021. 1519-6984 1678-4375 10.1590/1519-6984.253436 S1519-69842024000100158 S1519-69842024000100158.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128904286699520 |