Sexual development dysgenesis in interspecific hybrids of Medaka fish
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-09314-6 http://hdl.handle.net/11449/223731 |
Resumo: | Fish are amongst vertebrates the group with the highest diversity of known sex-determining genes. Particularly, the genus Oryzias is a suitable taxon to understand how different sex determination genetic networks evolved in closely related species. Two closely related species, O. latipes and O. curvinotus, do not only share the same XX/XY sex chromosome system, but also the same male sex-determining gene, dmrt1bY. We performed whole mRNA transcriptomes and morphology analyses of the gonads of hybrids resulting from reciprocal crosses between O. latipes and O. curvinotus. XY male hybrids, presenting meiotic arrest and no production of sperm were sterile, and about 30% of the XY hybrids underwent male-to-female sex reversal. Both XX and XY hybrid females exhibited reduced fertility and developed ovotestis while aging. Transcriptome data showed that male-related genes are upregulated in the XX and XY female hybrids. The transcriptomes of both types of female and of the male gonads are characterized by upregulation of meiosis and germ cell differentiation genes. Differences in the parental species in the downstream pathways of sexual development could explain sex reversal, sterility, and the development of intersex gonads in the hybrids. We hypothesize that male-to-female sex reversal may be connected to a different development time between species at which dmrt1bY expression starts. Our results provide molecular clues for the proximate mechanisms of hybrid incompatibility and Haldane’s rule. |
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Sexual development dysgenesis in interspecific hybrids of Medaka fishFish are amongst vertebrates the group with the highest diversity of known sex-determining genes. Particularly, the genus Oryzias is a suitable taxon to understand how different sex determination genetic networks evolved in closely related species. Two closely related species, O. latipes and O. curvinotus, do not only share the same XX/XY sex chromosome system, but also the same male sex-determining gene, dmrt1bY. We performed whole mRNA transcriptomes and morphology analyses of the gonads of hybrids resulting from reciprocal crosses between O. latipes and O. curvinotus. XY male hybrids, presenting meiotic arrest and no production of sperm were sterile, and about 30% of the XY hybrids underwent male-to-female sex reversal. Both XX and XY hybrid females exhibited reduced fertility and developed ovotestis while aging. Transcriptome data showed that male-related genes are upregulated in the XX and XY female hybrids. The transcriptomes of both types of female and of the male gonads are characterized by upregulation of meiosis and germ cell differentiation genes. Differences in the parental species in the downstream pathways of sexual development could explain sex reversal, sterility, and the development of intersex gonads in the hybrids. We hypothesize that male-to-female sex reversal may be connected to a different development time between species at which dmrt1bY expression starts. Our results provide molecular clues for the proximate mechanisms of hybrid incompatibility and Haldane’s rule.Julius-Maximilians-Universität WürzburgDeutsche ForschungsgemeinschaftDepartment of Developmental Biochemistry Biocenter University of Wuerzburg, Am HublandReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences UNESPBiochemistry and Cell Biology Biocenter University of Wuerzburg, Am HublandUR 1037 Fish Physiology and Genomics INRAEDepartment of Chemistry and Biochemistry The Xiphophorus Genetic Stock Center Texas State UniversityReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences UNESPDeutsche Forschungsgemeinschaft: 10-1Deutsche Forschungsgemeinschaft: Scha408/12-1University of WuerzburgUniversidade Estadual Paulista (UNESP)INRAETexas State UniversityMartinez-Bengochea, A. L. [UNESP]Kneitz, S.Herpin, A.Nóbrega, R. H. [UNESP]Adolfi, M. C.Schartl, M.2022-04-28T19:52:47Z2022-04-28T19:52:47Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-09314-6Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/22373110.1038/s41598-022-09314-62-s2.0-85127274869Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2022-04-28T19:52:47Zoai:repositorio.unesp.br:11449/223731Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:30:23.036258Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
title |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
spellingShingle |
Sexual development dysgenesis in interspecific hybrids of Medaka fish Martinez-Bengochea, A. L. [UNESP] |
title_short |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
title_full |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
title_fullStr |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
title_full_unstemmed |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
title_sort |
Sexual development dysgenesis in interspecific hybrids of Medaka fish |
author |
Martinez-Bengochea, A. L. [UNESP] |
author_facet |
Martinez-Bengochea, A. L. [UNESP] Kneitz, S. Herpin, A. Nóbrega, R. H. [UNESP] Adolfi, M. C. Schartl, M. |
author_role |
author |
author2 |
Kneitz, S. Herpin, A. Nóbrega, R. H. [UNESP] Adolfi, M. C. Schartl, M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
University of Wuerzburg Universidade Estadual Paulista (UNESP) INRAE Texas State University |
dc.contributor.author.fl_str_mv |
Martinez-Bengochea, A. L. [UNESP] Kneitz, S. Herpin, A. Nóbrega, R. H. [UNESP] Adolfi, M. C. Schartl, M. |
description |
Fish are amongst vertebrates the group with the highest diversity of known sex-determining genes. Particularly, the genus Oryzias is a suitable taxon to understand how different sex determination genetic networks evolved in closely related species. Two closely related species, O. latipes and O. curvinotus, do not only share the same XX/XY sex chromosome system, but also the same male sex-determining gene, dmrt1bY. We performed whole mRNA transcriptomes and morphology analyses of the gonads of hybrids resulting from reciprocal crosses between O. latipes and O. curvinotus. XY male hybrids, presenting meiotic arrest and no production of sperm were sterile, and about 30% of the XY hybrids underwent male-to-female sex reversal. Both XX and XY hybrid females exhibited reduced fertility and developed ovotestis while aging. Transcriptome data showed that male-related genes are upregulated in the XX and XY female hybrids. The transcriptomes of both types of female and of the male gonads are characterized by upregulation of meiosis and germ cell differentiation genes. Differences in the parental species in the downstream pathways of sexual development could explain sex reversal, sterility, and the development of intersex gonads in the hybrids. We hypothesize that male-to-female sex reversal may be connected to a different development time between species at which dmrt1bY expression starts. Our results provide molecular clues for the proximate mechanisms of hybrid incompatibility and Haldane’s rule. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:47Z 2022-04-28T19:52:47Z 2022-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-09314-6 Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/223731 10.1038/s41598-022-09314-6 2-s2.0-85127274869 |
url |
http://dx.doi.org/10.1038/s41598-022-09314-6 http://hdl.handle.net/11449/223731 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-09314-6 2-s2.0-85127274869 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129526945808384 |