Diferenciação hepatocítica de células-tronco mesenquimais da medula óssea humana

• Autor(a) principal: Penteado, F. C L [UNESP]
• Data de Publicação: 2007
• Outros Autores: Orellana, M. D., Fontes, A. M., Kashima, S., Covas, Dimas Tadeu
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/249; http://hdl.handle.net/11449/70132
• topic: Albumin; Cytokeratin 18; Hepatocyte differentiation; Immunodeficient mice; Liver damage; Mesenchymal stem cells; albumin; alpha fetoprotein; cytokeratin 18; animal cell; animal experiment; animal model; animal tissue; cell differentiation; cell isolation; cell migration; cell structure; controlled study; hematopoietic stem cell; human; human cell; immunofluorescence test; immunophenotyping; liver cell; liver injury; mesenchymal stem cell; mouse; nonhuman; normal human; paracrine signaling; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction

Some recent articles have reported that mesenchymal stem cells (MSCs) can be induced to express hepatocyte markers by transplanting them into animal models of liver damage, or by in vitro culture with growth factors and cytokines. In this study, the aim is to evaluate the behavior of MSCs subjected to induction of hepatocyte differentiation. The MSCs were isolated from the bone marrow of 4 normal donors, characterized and subjected to both in vitro and in vivo induction of hepatocyte differentiation. The in vitro induced cells showed morphological changes, acquiring hepatocyte-like features. However, the immunophenotype of these cells was not modified. The induced cells exhibited no increase in albumin, cytokeratin 18 or cytokeratin 19 transcripts, when analyzed by real-time RT-PCR. The expression of albumin, cytokeratin 18 and alpha fetoprotein was also unchanged, according to immunofluorescence tests. In vivo, the MSC demonstrated a potential to migrate to damaged liver tissue in immunodeficient mice. Taken together, the results suggest that bone marrow MSCs are incapable of in vitro differentiation into hepatocytes by the approach used here, but are capable of homing to damaged hepatic tissue in vivo, suggesting a role for them in the repair of the liver. This contribution to tissue repair could be associated with a paracrine effect exerted by these cells.