Specific lipolytic activity in fungus collected from sugarcane bagasse
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Publication Date: | 2021 |
Other Authors: | , , , |
Format: | Article |
Language: | por |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.17765/2176-9168.2021V14N2E8069 http://hdl.handle.net/11449/206362 |
Summary: | Lipases are enzymes which catalyze chemical reactions, especially lipidic bonds. Enzymes may be retrieved from lipolytic fungi which produce high rates of lipases from a carbon source. Fungus previously selected from sugarcane bagasse underwent submersed fermentation with extra-virgin olive oil as the carbon source. Broth produced by the fungus was analyzed by spectrophotometry with substrate p-NPB and results were analyzed at 5% level. Current assay identified fungus which was capable of growth in a mineral medium with sole lipid carbon source and quantifies specific activities of lipases by substrate p-NPB. Best rates of specific lipid activities of the three fungi were 8.004 U mg-1, 5.897 U mg-1, 5.077 U mg-1, for fungus Paecilomyces formosus (first isolate), Paecilomyces formosus (second isolate) and Aspergillus tubingensis, which are rates cited for the first time for the three fungi. When compared to other microorganisms in the literature, they are higher and more active to be used in industrial processes. |
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Specific lipolytic activity in fungus collected from sugarcane bagasseAtividade específica lipolítica em fungos coletados do bagaço de cana-de-açúcarAspergillus tubingensisLipasesMicrobiologyPaecilomyces formosusLipases are enzymes which catalyze chemical reactions, especially lipidic bonds. Enzymes may be retrieved from lipolytic fungi which produce high rates of lipases from a carbon source. Fungus previously selected from sugarcane bagasse underwent submersed fermentation with extra-virgin olive oil as the carbon source. Broth produced by the fungus was analyzed by spectrophotometry with substrate p-NPB and results were analyzed at 5% level. Current assay identified fungus which was capable of growth in a mineral medium with sole lipid carbon source and quantifies specific activities of lipases by substrate p-NPB. Best rates of specific lipid activities of the three fungi were 8.004 U mg-1, 5.897 U mg-1, 5.077 U mg-1, for fungus Paecilomyces formosus (first isolate), Paecilomyces formosus (second isolate) and Aspergillus tubingensis, which are rates cited for the first time for the three fungi. When compared to other microorganisms in the literature, they are higher and more active to be used in industrial processes.Instituto Federal de Educação Ciência e Tecnologia Goiano (IFG)Colégio Estadual Alfredo NasserUnespUnespCiência e Tecnologia Goiano (IFG)Colégio Estadual Alfredo NasserUniversidade Estadual Paulista (Unesp)Bessa, Dâmaris Hadassa Rangel Fonsecade Souza Castro, Carlos FredericoSouza, Amanda Oliveirade Menezes Filho, Antonio Carlos PereiraGonçalves, Mariana Costa Mello [UNESP]2021-06-25T10:30:47Z2021-06-25T10:30:47Z2021-03-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.17765/2176-9168.2021V14N2E8069Revista em Agronegocio e Meio Ambiente, v. 14, n. 2, 2021.2176-91681981-9951http://hdl.handle.net/11449/20636210.17765/2176-9168.2021V14N2E80692-s2.0-85106227423Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista em Agronegocio e Meio Ambienteinfo:eu-repo/semantics/openAccess2021-10-23T04:16:26Zoai:repositorio.unesp.br:11449/206362Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:45:37.650858Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Specific lipolytic activity in fungus collected from sugarcane bagasse Atividade específica lipolítica em fungos coletados do bagaço de cana-de-açúcar |
title |
Specific lipolytic activity in fungus collected from sugarcane bagasse |
spellingShingle |
Specific lipolytic activity in fungus collected from sugarcane bagasse Bessa, Dâmaris Hadassa Rangel Fonseca Aspergillus tubingensis Lipases Microbiology Paecilomyces formosus |
title_short |
Specific lipolytic activity in fungus collected from sugarcane bagasse |
title_full |
Specific lipolytic activity in fungus collected from sugarcane bagasse |
title_fullStr |
Specific lipolytic activity in fungus collected from sugarcane bagasse |
title_full_unstemmed |
Specific lipolytic activity in fungus collected from sugarcane bagasse |
title_sort |
Specific lipolytic activity in fungus collected from sugarcane bagasse |
author |
Bessa, Dâmaris Hadassa Rangel Fonseca |
author_facet |
Bessa, Dâmaris Hadassa Rangel Fonseca de Souza Castro, Carlos Frederico Souza, Amanda Oliveira de Menezes Filho, Antonio Carlos Pereira Gonçalves, Mariana Costa Mello [UNESP] |
author_role |
author |
author2 |
de Souza Castro, Carlos Frederico Souza, Amanda Oliveira de Menezes Filho, Antonio Carlos Pereira Gonçalves, Mariana Costa Mello [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Ciência e Tecnologia Goiano (IFG) Colégio Estadual Alfredo Nasser Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bessa, Dâmaris Hadassa Rangel Fonseca de Souza Castro, Carlos Frederico Souza, Amanda Oliveira de Menezes Filho, Antonio Carlos Pereira Gonçalves, Mariana Costa Mello [UNESP] |
dc.subject.por.fl_str_mv |
Aspergillus tubingensis Lipases Microbiology Paecilomyces formosus |
topic |
Aspergillus tubingensis Lipases Microbiology Paecilomyces formosus |
description |
Lipases are enzymes which catalyze chemical reactions, especially lipidic bonds. Enzymes may be retrieved from lipolytic fungi which produce high rates of lipases from a carbon source. Fungus previously selected from sugarcane bagasse underwent submersed fermentation with extra-virgin olive oil as the carbon source. Broth produced by the fungus was analyzed by spectrophotometry with substrate p-NPB and results were analyzed at 5% level. Current assay identified fungus which was capable of growth in a mineral medium with sole lipid carbon source and quantifies specific activities of lipases by substrate p-NPB. Best rates of specific lipid activities of the three fungi were 8.004 U mg-1, 5.897 U mg-1, 5.077 U mg-1, for fungus Paecilomyces formosus (first isolate), Paecilomyces formosus (second isolate) and Aspergillus tubingensis, which are rates cited for the first time for the three fungi. When compared to other microorganisms in the literature, they are higher and more active to be used in industrial processes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:30:47Z 2021-06-25T10:30:47Z 2021-03-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.17765/2176-9168.2021V14N2E8069 Revista em Agronegocio e Meio Ambiente, v. 14, n. 2, 2021. 2176-9168 1981-9951 http://hdl.handle.net/11449/206362 10.17765/2176-9168.2021V14N2E8069 2-s2.0-85106227423 |
url |
http://dx.doi.org/10.17765/2176-9168.2021V14N2E8069 http://hdl.handle.net/11449/206362 |
identifier_str_mv |
Revista em Agronegocio e Meio Ambiente, v. 14, n. 2, 2021. 2176-9168 1981-9951 10.17765/2176-9168.2021V14N2E8069 2-s2.0-85106227423 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista em Agronegocio e Meio Ambiente |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129355240439808 |