Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/aob/mcz025 http://hdl.handle.net/11449/221324 |
Resumo: | Background and Aims: Juvenile-to-adult phase transition is marked by changes in leaf morphology, mostly due to the temporal development of the shoot apical meristem, a phenomenon known as heteroblasty. Sugars and microRNA-controlled modules are components of the heteroblastic process in Arabidopsis thaliana leaves. However, our understanding about their roles during phase-changing in other species, such as Passiflora edulis, remains limited. Unlike Arabidopsis, P. edulis (a semi-woody perennial climbing vine) undergoes remarkable changes in leaf morphology throughout juvenile-to-adult transition. Nonetheless, the underlying molecular mechanisms are unknown. Methods: Here we evaluated the molecular mechanisms underlying the heteroblastic process by analysing the temporal expression of microRNAs and targets in leaves as well as the leaf metabolome during P. edulis development. Key Results: Metabolic profiling revealed a unique composition of metabolites associated with leaf heteroblasty. Increasing levels of glucose and α-trehalose were observed during juvenile-to-adult phase transition. Accumulation of microRNA156 (miR156) correlated with juvenile leaf traits, whilst miR172 transcript accumulation was associated with leaf adult traits. Importantly, glucose may mediate adult leaf characteristics during de novo shoot organogenesis by modulating miR156-targeted PeSPL9 expression levels at early stages of shoot development. Conclusions: Altogether, our results suggest that specific sugars may act as co-regulators, along with two microRNAs, leading to leaf morphological modifications throughout juvenile-to-adult phase transition in P. edulis. |
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Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172de novo shoot organogenesisHeteroblastymicroRNAPassiflora edulissugarBackground and Aims: Juvenile-to-adult phase transition is marked by changes in leaf morphology, mostly due to the temporal development of the shoot apical meristem, a phenomenon known as heteroblasty. Sugars and microRNA-controlled modules are components of the heteroblastic process in Arabidopsis thaliana leaves. However, our understanding about their roles during phase-changing in other species, such as Passiflora edulis, remains limited. Unlike Arabidopsis, P. edulis (a semi-woody perennial climbing vine) undergoes remarkable changes in leaf morphology throughout juvenile-to-adult transition. Nonetheless, the underlying molecular mechanisms are unknown. Methods: Here we evaluated the molecular mechanisms underlying the heteroblastic process by analysing the temporal expression of microRNAs and targets in leaves as well as the leaf metabolome during P. edulis development. Key Results: Metabolic profiling revealed a unique composition of metabolites associated with leaf heteroblasty. Increasing levels of glucose and α-trehalose were observed during juvenile-to-adult phase transition. Accumulation of microRNA156 (miR156) correlated with juvenile leaf traits, whilst miR172 transcript accumulation was associated with leaf adult traits. Importantly, glucose may mediate adult leaf characteristics during de novo shoot organogenesis by modulating miR156-targeted PeSPL9 expression levels at early stages of shoot development. Conclusions: Altogether, our results suggest that specific sugars may act as co-regulators, along with two microRNAs, leading to leaf morphological modifications throughout juvenile-to-adult phase transition in P. edulis.Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada A Agropecuária (BIOAGRO) Universidade Federal de ViçosaUniversidade Estadual Do MaranhãoDepartamento de Biologia Vegetal Universidade Federal de ViçosaInstituto de Educação Agricultura e Ambiente Universidade Federal Do AmazonasEscola Superior de Agricultura Luiz de Queiroz Universidade de São PauloInstituto de Biociências Universidade Estadual de São PauloInstituto de Biociências Universidade Estadual de São PauloUniversidade Federal de Viçosa (UFV)Universidade Estadual Do MaranhãoUniversidade Federal Do AmazonasUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Silva, Priscila OBatista, DIego SCavalcanti, João Henrique FKoehler, Andréa DVieira, Lorena MFernandes, Amanda MBarrera-Rojas, Carlos Hernan [UNESP]Ribeiro, DImas MNogueira, Fabio T. SOtoni, Wagner C2022-04-28T19:27:27Z2022-04-28T19:27:27Z2019-07-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1191-1203http://dx.doi.org/10.1093/aob/mcz025Annals of Botany, v. 123, n. 7, p. 1191-1203, 2019.1095-82900305-7364http://hdl.handle.net/11449/22132410.1093/aob/mcz0252-s2.0-85069287904Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnnals of Botanyinfo:eu-repo/semantics/openAccess2022-04-28T19:27:27Zoai:repositorio.unesp.br:11449/221324Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:34:16.886433Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
title |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
spellingShingle |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 Silva, Priscila O de novo shoot organogenesis Heteroblasty microRNA Passiflora edulis sugar |
title_short |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
title_full |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
title_fullStr |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
title_full_unstemmed |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
title_sort |
Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172 |
author |
Silva, Priscila O |
author_facet |
Silva, Priscila O Batista, DIego S Cavalcanti, João Henrique F Koehler, Andréa D Vieira, Lorena M Fernandes, Amanda M Barrera-Rojas, Carlos Hernan [UNESP] Ribeiro, DImas M Nogueira, Fabio T. S Otoni, Wagner C |
author_role |
author |
author2 |
Batista, DIego S Cavalcanti, João Henrique F Koehler, Andréa D Vieira, Lorena M Fernandes, Amanda M Barrera-Rojas, Carlos Hernan [UNESP] Ribeiro, DImas M Nogueira, Fabio T. S Otoni, Wagner C |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Viçosa (UFV) Universidade Estadual Do Maranhão Universidade Federal Do Amazonas Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, Priscila O Batista, DIego S Cavalcanti, João Henrique F Koehler, Andréa D Vieira, Lorena M Fernandes, Amanda M Barrera-Rojas, Carlos Hernan [UNESP] Ribeiro, DImas M Nogueira, Fabio T. S Otoni, Wagner C |
dc.subject.por.fl_str_mv |
de novo shoot organogenesis Heteroblasty microRNA Passiflora edulis sugar |
topic |
de novo shoot organogenesis Heteroblasty microRNA Passiflora edulis sugar |
description |
Background and Aims: Juvenile-to-adult phase transition is marked by changes in leaf morphology, mostly due to the temporal development of the shoot apical meristem, a phenomenon known as heteroblasty. Sugars and microRNA-controlled modules are components of the heteroblastic process in Arabidopsis thaliana leaves. However, our understanding about their roles during phase-changing in other species, such as Passiflora edulis, remains limited. Unlike Arabidopsis, P. edulis (a semi-woody perennial climbing vine) undergoes remarkable changes in leaf morphology throughout juvenile-to-adult transition. Nonetheless, the underlying molecular mechanisms are unknown. Methods: Here we evaluated the molecular mechanisms underlying the heteroblastic process by analysing the temporal expression of microRNAs and targets in leaves as well as the leaf metabolome during P. edulis development. Key Results: Metabolic profiling revealed a unique composition of metabolites associated with leaf heteroblasty. Increasing levels of glucose and α-trehalose were observed during juvenile-to-adult phase transition. Accumulation of microRNA156 (miR156) correlated with juvenile leaf traits, whilst miR172 transcript accumulation was associated with leaf adult traits. Importantly, glucose may mediate adult leaf characteristics during de novo shoot organogenesis by modulating miR156-targeted PeSPL9 expression levels at early stages of shoot development. Conclusions: Altogether, our results suggest that specific sugars may act as co-regulators, along with two microRNAs, leading to leaf morphological modifications throughout juvenile-to-adult phase transition in P. edulis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-08 2022-04-28T19:27:27Z 2022-04-28T19:27:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/aob/mcz025 Annals of Botany, v. 123, n. 7, p. 1191-1203, 2019. 1095-8290 0305-7364 http://hdl.handle.net/11449/221324 10.1093/aob/mcz025 2-s2.0-85069287904 |
url |
http://dx.doi.org/10.1093/aob/mcz025 http://hdl.handle.net/11449/221324 |
identifier_str_mv |
Annals of Botany, v. 123, n. 7, p. 1191-1203, 2019. 1095-8290 0305-7364 10.1093/aob/mcz025 2-s2.0-85069287904 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Annals of Botany |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1191-1203 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128534122594304 |