Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00056-019-00197-3 http://hdl.handle.net/11449/199553 |
Resumo: | Purpose: Orthodontic treatment is based on the principle of force application to teeth and subsequently to the surrounding tissues and periodontal cells. Sequestosome 1 (SQSTM1) is a well-known marker for autophagy, which is an important cellular mechanism of adaptation to stress. The aim of this study was to analyze whether biomechanical loading conditions regulate SQSTM1 in periodontal cells and tissues, thereby providing further information on the role of autophagy in orthodontic tooth movement. Methods: Periodontal ligament (PDL) fibroblasts were exposed to cyclic tensile strain of low magnitude (3%, CTSL), and the regulation of autophagy-associated targets was determined with an array-based approach. SQSTM1 was selected for further biomechanical loading experiments with dynamic and static tensile strain and assessed via real-time polymerase chain reaction (RT-PCR) and immunoblotting. Signaling pathways involved in SQSTM1 activation were analyzed by using specific inhibitors, including an autophagy inhibitor. Finally, SQSTM1 expression was analyzed in gingival biopsies and histological sections of rats in presence and absence of orthodontic forces. Results: Multiple autophagy-associated targets were regulated by CTSL in PDL fibroblasts. All biomechanical loading conditions tested increased the SQSTM1 expression significantly. Stimulatory effects of CTSL on SQSTM1 expression were diminished by inhibition of the c‑Jun N‑terminal kinase (JNK) pathway and of autophagy. Increased SQSTM1 levels after CTSL were confirmed by immunoblotting. Orthodontic force application also led to significantly elevated SQTSM1 levels in the gingiva and PDL of treated animals as compared to control. Conclusions: Our in vitro and in vivo findings provide evidence of a role of SQSTM1 and thereby autophagy in orthodontic tooth movement. |
id |
UNSP_92b6477b4c9cff61f0aa94adea8f2d0d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/199553 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loadingAutophagyMechanical stressOrthodontic tooth movementPeriodontal ligamentSequestosome 1Purpose: Orthodontic treatment is based on the principle of force application to teeth and subsequently to the surrounding tissues and periodontal cells. Sequestosome 1 (SQSTM1) is a well-known marker for autophagy, which is an important cellular mechanism of adaptation to stress. The aim of this study was to analyze whether biomechanical loading conditions regulate SQSTM1 in periodontal cells and tissues, thereby providing further information on the role of autophagy in orthodontic tooth movement. Methods: Periodontal ligament (PDL) fibroblasts were exposed to cyclic tensile strain of low magnitude (3%, CTSL), and the regulation of autophagy-associated targets was determined with an array-based approach. SQSTM1 was selected for further biomechanical loading experiments with dynamic and static tensile strain and assessed via real-time polymerase chain reaction (RT-PCR) and immunoblotting. Signaling pathways involved in SQSTM1 activation were analyzed by using specific inhibitors, including an autophagy inhibitor. Finally, SQSTM1 expression was analyzed in gingival biopsies and histological sections of rats in presence and absence of orthodontic forces. Results: Multiple autophagy-associated targets were regulated by CTSL in PDL fibroblasts. All biomechanical loading conditions tested increased the SQSTM1 expression significantly. Stimulatory effects of CTSL on SQSTM1 expression were diminished by inhibition of the c‑Jun N‑terminal kinase (JNK) pathway and of autophagy. Increased SQSTM1 levels after CTSL were confirmed by immunoblotting. Orthodontic force application also led to significantly elevated SQTSM1 levels in the gingiva and PDL of treated animals as compared to control. Conclusions: Our in vitro and in vivo findings provide evidence of a role of SQSTM1 and thereby autophagy in orthodontic tooth movement.Department of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstr. 17Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of BonnDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg UniversityDepartment of Orthodontics and Orofacial Orthopedics University of ErlangenInstitute of Human Genetics University Hospital of BonnDepartment of Diagnosis and Surgery School of Dentistry at Araraquara University Estadual Paulista—UNESPInstitute of Reconstructive Neurobiology Life and Brain Center University of BonnDepartment of Diagnosis and Surgery School of Dentistry at Araraquara University Estadual Paulista—UNESPUniversity of BonnUniversity Medical Center of the Johannes Gutenberg UniversityUniversity of ErlangenUniversity Hospital of BonnUniversidade Estadual Paulista (Unesp)Memmert, S.Nogueira, A. V.B.Damanaki, A.Nokhbehsaim, M.Rath-Deschner, B.Götz, W.Gölz, L.Cirelli, J. A. [UNESP]Till, A.Jäger, A.Deschner, J.2020-12-12T01:43:02Z2020-12-12T01:43:02Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10-21http://dx.doi.org/10.1007/s00056-019-00197-3Journal of Orofacial Orthopedics, v. 81, n. 1, p. 10-21, 2020.1434-5293http://hdl.handle.net/11449/19955310.1007/s00056-019-00197-32-s2.0-85073964041Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Orofacial Orthopedicsinfo:eu-repo/semantics/openAccess2021-10-23T08:05:18Zoai:repositorio.unesp.br:11449/199553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:26.019612Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
title |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
spellingShingle |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading Memmert, S. Autophagy Mechanical stress Orthodontic tooth movement Periodontal ligament Sequestosome 1 |
title_short |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
title_full |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
title_fullStr |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
title_full_unstemmed |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
title_sort |
Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading |
author |
Memmert, S. |
author_facet |
Memmert, S. Nogueira, A. V.B. Damanaki, A. Nokhbehsaim, M. Rath-Deschner, B. Götz, W. Gölz, L. Cirelli, J. A. [UNESP] Till, A. Jäger, A. Deschner, J. |
author_role |
author |
author2 |
Nogueira, A. V.B. Damanaki, A. Nokhbehsaim, M. Rath-Deschner, B. Götz, W. Gölz, L. Cirelli, J. A. [UNESP] Till, A. Jäger, A. Deschner, J. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Bonn University Medical Center of the Johannes Gutenberg University University of Erlangen University Hospital of Bonn Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Memmert, S. Nogueira, A. V.B. Damanaki, A. Nokhbehsaim, M. Rath-Deschner, B. Götz, W. Gölz, L. Cirelli, J. A. [UNESP] Till, A. Jäger, A. Deschner, J. |
dc.subject.por.fl_str_mv |
Autophagy Mechanical stress Orthodontic tooth movement Periodontal ligament Sequestosome 1 |
topic |
Autophagy Mechanical stress Orthodontic tooth movement Periodontal ligament Sequestosome 1 |
description |
Purpose: Orthodontic treatment is based on the principle of force application to teeth and subsequently to the surrounding tissues and periodontal cells. Sequestosome 1 (SQSTM1) is a well-known marker for autophagy, which is an important cellular mechanism of adaptation to stress. The aim of this study was to analyze whether biomechanical loading conditions regulate SQSTM1 in periodontal cells and tissues, thereby providing further information on the role of autophagy in orthodontic tooth movement. Methods: Periodontal ligament (PDL) fibroblasts were exposed to cyclic tensile strain of low magnitude (3%, CTSL), and the regulation of autophagy-associated targets was determined with an array-based approach. SQSTM1 was selected for further biomechanical loading experiments with dynamic and static tensile strain and assessed via real-time polymerase chain reaction (RT-PCR) and immunoblotting. Signaling pathways involved in SQSTM1 activation were analyzed by using specific inhibitors, including an autophagy inhibitor. Finally, SQSTM1 expression was analyzed in gingival biopsies and histological sections of rats in presence and absence of orthodontic forces. Results: Multiple autophagy-associated targets were regulated by CTSL in PDL fibroblasts. All biomechanical loading conditions tested increased the SQSTM1 expression significantly. Stimulatory effects of CTSL on SQSTM1 expression were diminished by inhibition of the c‑Jun N‑terminal kinase (JNK) pathway and of autophagy. Increased SQSTM1 levels after CTSL were confirmed by immunoblotting. Orthodontic force application also led to significantly elevated SQTSM1 levels in the gingiva and PDL of treated animals as compared to control. Conclusions: Our in vitro and in vivo findings provide evidence of a role of SQSTM1 and thereby autophagy in orthodontic tooth movement. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:43:02Z 2020-12-12T01:43:02Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00056-019-00197-3 Journal of Orofacial Orthopedics, v. 81, n. 1, p. 10-21, 2020. 1434-5293 http://hdl.handle.net/11449/199553 10.1007/s00056-019-00197-3 2-s2.0-85073964041 |
url |
http://dx.doi.org/10.1007/s00056-019-00197-3 http://hdl.handle.net/11449/199553 |
identifier_str_mv |
Journal of Orofacial Orthopedics, v. 81, n. 1, p. 10-21, 2020. 1434-5293 10.1007/s00056-019-00197-3 2-s2.0-85073964041 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Orofacial Orthopedics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10-21 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129101172572160 |