Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2018.04.008 http://hdl.handle.net/11449/166196 |
Resumo: | Bovine periodontitis is a progressive and purulent infection associated with an anaerobic subgingival biofilm, which induces irreversible damage to the dentition of affected animals. The aetiopathogenesis of the disease is unclear and treatment and control of the disease process in cattle are almost unknown. The aim of this study was to investigate the innate immune response by quantifying expression of Toll-like receptor (TLR) and cytokine genes in gingival tissue samples from cattle with and without periodontitis. Postmortem biopsies of gingival tissues were collected from 20 cattle with periodontitis and 20 cattle with no clinical signs of periodontal lesions. Tissue expression of TLR2, TLR4, TNF-alpha, IFN-gamma, IL-1 beta and IL-4 genes were determined using quantitative real-time PCR. Statistically significant increases in mRNA levels encoding TLR2 (p = 0.025), TLR4 (p = 0.037), TNF-alpha (p = 0.025), IFN-gamma (p = 0.014), IL-1 beta (p < 0.001) and IL-4 (p = 0.014) were observed in animals with periodontitis when compared to periodontally healthy animals. Increased levels of TLRs and inflammatory cytokines in periodontal tissue indicate an induction of the innate immune response of cattle and suggest that a substantial microbial challenge may be involved in the aetiopathogenesis of bovine periodontitis. |
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Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral healthBovinePeriodontitisToll-like receptorsCytokinesInnate immune responseBovine periodontitis is a progressive and purulent infection associated with an anaerobic subgingival biofilm, which induces irreversible damage to the dentition of affected animals. The aetiopathogenesis of the disease is unclear and treatment and control of the disease process in cattle are almost unknown. The aim of this study was to investigate the innate immune response by quantifying expression of Toll-like receptor (TLR) and cytokine genes in gingival tissue samples from cattle with and without periodontitis. Postmortem biopsies of gingival tissues were collected from 20 cattle with periodontitis and 20 cattle with no clinical signs of periodontal lesions. Tissue expression of TLR2, TLR4, TNF-alpha, IFN-gamma, IL-1 beta and IL-4 genes were determined using quantitative real-time PCR. Statistically significant increases in mRNA levels encoding TLR2 (p = 0.025), TLR4 (p = 0.037), TNF-alpha (p = 0.025), IFN-gamma (p = 0.014), IL-1 beta (p < 0.001) and IL-4 (p = 0.014) were observed in animals with periodontitis when compared to periodontally healthy animals. Increased levels of TLRs and inflammatory cytokines in periodontal tissue indicate an induction of the innate immune response of cattle and suggest that a substantial microbial challenge may be involved in the aetiopathogenesis of bovine periodontitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Vet Med, Dept Anim Hlth & Prod, Sao Paulo, BrazilUniv Glasgow, Sch Dent, Oral Sci Res Grp, Glasgow, Lanark, ScotlandUniv Glasgow, Sch Vet Med, Glasgow, Lanark, ScotlandUniv Estadual Paulista, Fac Vet Med, Dept Anim Hlth & Prod, Sao Paulo, BrazilFAPESP: FAPESP2015/06917-9Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ GlasgowBorsanelli, Ana C. [UNESP]Lappin, David F.Viora, LorenzoKing, GeorgeBennett, DavidDutra, Iveraldo S. [UNESP]Riggio, Marcello P.2018-11-29T18:48:45Z2018-11-29T18:48:45Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article439-443application/pdfhttp://dx.doi.org/10.1016/j.rvsc.2018.04.008Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 118, p. 439-443, 2018.0034-5288http://hdl.handle.net/11449/16619610.1016/j.rvsc.2018.04.008WOS:000435059400057WOS000435059400057.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch In Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-09-04T19:16:19Zoai:repositorio.unesp.br:11449/166196Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
title |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
spellingShingle |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health Borsanelli, Ana C. [UNESP] Bovine Periodontitis Toll-like receptors Cytokines Innate immune response |
title_short |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
title_full |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
title_fullStr |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
title_full_unstemmed |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
title_sort |
Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health |
author |
Borsanelli, Ana C. [UNESP] |
author_facet |
Borsanelli, Ana C. [UNESP] Lappin, David F. Viora, Lorenzo King, George Bennett, David Dutra, Iveraldo S. [UNESP] Riggio, Marcello P. |
author_role |
author |
author2 |
Lappin, David F. Viora, Lorenzo King, George Bennett, David Dutra, Iveraldo S. [UNESP] Riggio, Marcello P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Glasgow |
dc.contributor.author.fl_str_mv |
Borsanelli, Ana C. [UNESP] Lappin, David F. Viora, Lorenzo King, George Bennett, David Dutra, Iveraldo S. [UNESP] Riggio, Marcello P. |
dc.subject.por.fl_str_mv |
Bovine Periodontitis Toll-like receptors Cytokines Innate immune response |
topic |
Bovine Periodontitis Toll-like receptors Cytokines Innate immune response |
description |
Bovine periodontitis is a progressive and purulent infection associated with an anaerobic subgingival biofilm, which induces irreversible damage to the dentition of affected animals. The aetiopathogenesis of the disease is unclear and treatment and control of the disease process in cattle are almost unknown. The aim of this study was to investigate the innate immune response by quantifying expression of Toll-like receptor (TLR) and cytokine genes in gingival tissue samples from cattle with and without periodontitis. Postmortem biopsies of gingival tissues were collected from 20 cattle with periodontitis and 20 cattle with no clinical signs of periodontal lesions. Tissue expression of TLR2, TLR4, TNF-alpha, IFN-gamma, IL-1 beta and IL-4 genes were determined using quantitative real-time PCR. Statistically significant increases in mRNA levels encoding TLR2 (p = 0.025), TLR4 (p = 0.037), TNF-alpha (p = 0.025), IFN-gamma (p = 0.014), IL-1 beta (p < 0.001) and IL-4 (p = 0.014) were observed in animals with periodontitis when compared to periodontally healthy animals. Increased levels of TLRs and inflammatory cytokines in periodontal tissue indicate an induction of the innate immune response of cattle and suggest that a substantial microbial challenge may be involved in the aetiopathogenesis of bovine periodontitis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-29T18:48:45Z 2018-11-29T18:48:45Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2018.04.008 Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 118, p. 439-443, 2018. 0034-5288 http://hdl.handle.net/11449/166196 10.1016/j.rvsc.2018.04.008 WOS:000435059400057 WOS000435059400057.pdf |
url |
http://dx.doi.org/10.1016/j.rvsc.2018.04.008 http://hdl.handle.net/11449/166196 |
identifier_str_mv |
Research In Veterinary Science. Oxford: Elsevier Sci Ltd, v. 118, p. 439-443, 2018. 0034-5288 10.1016/j.rvsc.2018.04.008 WOS:000435059400057 WOS000435059400057.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research In Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
439-443 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021421176324096 |