Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s13071-022-05219-5 http://hdl.handle.net/11449/223726 |
Resumo: | Background: In Brazil, malaria is concentrated in the Amazon Basin, where more than 99% of the annual cases are reported. The main goal of this study was to investigate the population structure and genetic association of the biting behavior of Nyssorhynchus (also known as Anopheles) darlingi, the major malaria vector in the Amazon region of Brazil, using low-coverage genomic sequencing data. Methods: Samples were collected in the municipality of Mâncio Lima, Acre state, Brazil between 2016 and 2017. Different approaches using genotype imputation and no gene imputation for data treatment and low-coverage sequencing genotyping were performed. After the samples were genotyped, population stratification analysis was performed. Results: Weak but statistically significant stratification signatures were identified between subpopulations separated by distances of approximately 2–3 km. Genome-wide association studies (GWAS) were performed to compare indoor/outdoor biting behavior and blood-seeking at dusk/dawn. A statistically significant association was observed between biting behavior and single nucleotide polymorphism (SNP) markers adjacent to the gene associated with cytochrome P450 (CYP) 4H14, which is associated with insecticide resistance. A statistically significant association between blood-seeking periodicity and SNP markers adjacent to genes associated with the circadian cycle was also observed. Conclusion: The data presented here suggest that low-coverage whole-genome sequencing with adequate processing is a powerful tool to genetically characterize vector populations at a microgeographic scale in malaria transmission areas, as well as for use in GWAS. Female mosquitoes entering houses to take a blood meal may be related to a specific CYP4H14 allele, and female timing of blood-seeking is related to circadian rhythm genes. Graphical Abstract: [Figure not available: see fulltext.] |
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Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behaviorBackground: In Brazil, malaria is concentrated in the Amazon Basin, where more than 99% of the annual cases are reported. The main goal of this study was to investigate the population structure and genetic association of the biting behavior of Nyssorhynchus (also known as Anopheles) darlingi, the major malaria vector in the Amazon region of Brazil, using low-coverage genomic sequencing data. Methods: Samples were collected in the municipality of Mâncio Lima, Acre state, Brazil between 2016 and 2017. Different approaches using genotype imputation and no gene imputation for data treatment and low-coverage sequencing genotyping were performed. After the samples were genotyped, population stratification analysis was performed. Results: Weak but statistically significant stratification signatures were identified between subpopulations separated by distances of approximately 2–3 km. Genome-wide association studies (GWAS) were performed to compare indoor/outdoor biting behavior and blood-seeking at dusk/dawn. A statistically significant association was observed between biting behavior and single nucleotide polymorphism (SNP) markers adjacent to the gene associated with cytochrome P450 (CYP) 4H14, which is associated with insecticide resistance. A statistically significant association between blood-seeking periodicity and SNP markers adjacent to genes associated with the circadian cycle was also observed. Conclusion: The data presented here suggest that low-coverage whole-genome sequencing with adequate processing is a powerful tool to genetically characterize vector populations at a microgeographic scale in malaria transmission areas, as well as for use in GWAS. Female mosquitoes entering houses to take a blood meal may be related to a specific CYP4H14 allele, and female timing of blood-seeking is related to circadian rhythm genes. Graphical Abstract: [Figure not available: see fulltext.]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fogarty International CenterSao Paulo State University (UNESP)Nucleo de Medicina Tropical Universidade de BrasíliaLaboratorio ICEMR-Amazonia Laboratorios de Investigación Y Desarrollo Facultad de Ciencias Y Filosofia Universidad Peruana Cayetano HerediaFacultad de Salud Pública Universidad Peruana Cayetano HerediaDepartment of Immunology and Infection London School of Hygiene and Tropical MedicineDepartamento de Ciencias Celulares Y Moleculares Facultad de Ciencias Y Filosofía Universidad Peruana Cayetano HerediaInstituto de Medicina Tropical Alexander Von Humboldt Universidad Peruana Cayetano HerediaSection of Infectious Diseases Department of Internal Medicine Yale School of MedicineWadsworth Center New York State Department of HealthDepartment of Biomedical Sciences School of Public Health State University of New York at AlbanySao Paulo State University (UNESP)FAPESP: 2018/07406-6Fogarty International Center: D43 TW007120Universidade Estadual Paulista (UNESP)Universidade de Brasília (UnB)Universidad Peruana Cayetano HerediaLondon School of Hygiene and Tropical MedicineYale School of MedicineNew York State Department of HealthState University of New York at AlbanyAlvarez, Marcus Vinicius Niz [UNESP]Alonso, Diego Peres [UNESP]Kadri, Samir Moura [UNESP]Rufalco-Moutinho, PauloBernardes, Isabella Ariadne Ferrari [UNESP]de Mello, Ana Carolina Florindo [UNESP]Souto, Ana Carolina [UNESP]Carrasco-Escobar, GabrielMoreno, MartaGamboa, DioniciaVinetz, Joseph M.Conn, Jan E.Ribolla, Paulo E. M. [UNESP]2022-04-28T19:52:46Z2022-04-28T19:52:46Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s13071-022-05219-5Parasites and Vectors, v. 15, n. 1, 2022.1756-3305http://hdl.handle.net/11449/22372610.1186/s13071-022-05219-52-s2.0-85127246482Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasites and Vectorsinfo:eu-repo/semantics/openAccess2022-04-28T19:52:46Zoai:repositorio.unesp.br:11449/223726Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:16:34.123651Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
title |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
spellingShingle |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior Alvarez, Marcus Vinicius Niz [UNESP] |
title_short |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
title_full |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
title_fullStr |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
title_full_unstemmed |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
title_sort |
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior |
author |
Alvarez, Marcus Vinicius Niz [UNESP] |
author_facet |
Alvarez, Marcus Vinicius Niz [UNESP] Alonso, Diego Peres [UNESP] Kadri, Samir Moura [UNESP] Rufalco-Moutinho, Paulo Bernardes, Isabella Ariadne Ferrari [UNESP] de Mello, Ana Carolina Florindo [UNESP] Souto, Ana Carolina [UNESP] Carrasco-Escobar, Gabriel Moreno, Marta Gamboa, Dionicia Vinetz, Joseph M. Conn, Jan E. Ribolla, Paulo E. M. [UNESP] |
author_role |
author |
author2 |
Alonso, Diego Peres [UNESP] Kadri, Samir Moura [UNESP] Rufalco-Moutinho, Paulo Bernardes, Isabella Ariadne Ferrari [UNESP] de Mello, Ana Carolina Florindo [UNESP] Souto, Ana Carolina [UNESP] Carrasco-Escobar, Gabriel Moreno, Marta Gamboa, Dionicia Vinetz, Joseph M. Conn, Jan E. Ribolla, Paulo E. M. [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de Brasília (UnB) Universidad Peruana Cayetano Heredia London School of Hygiene and Tropical Medicine Yale School of Medicine New York State Department of Health State University of New York at Albany |
dc.contributor.author.fl_str_mv |
Alvarez, Marcus Vinicius Niz [UNESP] Alonso, Diego Peres [UNESP] Kadri, Samir Moura [UNESP] Rufalco-Moutinho, Paulo Bernardes, Isabella Ariadne Ferrari [UNESP] de Mello, Ana Carolina Florindo [UNESP] Souto, Ana Carolina [UNESP] Carrasco-Escobar, Gabriel Moreno, Marta Gamboa, Dionicia Vinetz, Joseph M. Conn, Jan E. Ribolla, Paulo E. M. [UNESP] |
description |
Background: In Brazil, malaria is concentrated in the Amazon Basin, where more than 99% of the annual cases are reported. The main goal of this study was to investigate the population structure and genetic association of the biting behavior of Nyssorhynchus (also known as Anopheles) darlingi, the major malaria vector in the Amazon region of Brazil, using low-coverage genomic sequencing data. Methods: Samples were collected in the municipality of Mâncio Lima, Acre state, Brazil between 2016 and 2017. Different approaches using genotype imputation and no gene imputation for data treatment and low-coverage sequencing genotyping were performed. After the samples were genotyped, population stratification analysis was performed. Results: Weak but statistically significant stratification signatures were identified between subpopulations separated by distances of approximately 2–3 km. Genome-wide association studies (GWAS) were performed to compare indoor/outdoor biting behavior and blood-seeking at dusk/dawn. A statistically significant association was observed between biting behavior and single nucleotide polymorphism (SNP) markers adjacent to the gene associated with cytochrome P450 (CYP) 4H14, which is associated with insecticide resistance. A statistically significant association between blood-seeking periodicity and SNP markers adjacent to genes associated with the circadian cycle was also observed. Conclusion: The data presented here suggest that low-coverage whole-genome sequencing with adequate processing is a powerful tool to genetically characterize vector populations at a microgeographic scale in malaria transmission areas, as well as for use in GWAS. Female mosquitoes entering houses to take a blood meal may be related to a specific CYP4H14 allele, and female timing of blood-seeking is related to circadian rhythm genes. Graphical Abstract: [Figure not available: see fulltext.] |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:46Z 2022-04-28T19:52:46Z 2022-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s13071-022-05219-5 Parasites and Vectors, v. 15, n. 1, 2022. 1756-3305 http://hdl.handle.net/11449/223726 10.1186/s13071-022-05219-5 2-s2.0-85127246482 |
url |
http://dx.doi.org/10.1186/s13071-022-05219-5 http://hdl.handle.net/11449/223726 |
identifier_str_mv |
Parasites and Vectors, v. 15, n. 1, 2022. 1756-3305 10.1186/s13071-022-05219-5 2-s2.0-85127246482 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasites and Vectors |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128782104526848 |