Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fphar.2021.715394 http://hdl.handle.net/11449/231526 |
Resumo: | Although fluoride (F) is well-known to prevent dental caries, changes in cell processes in different tissues have been associated with its excessive exposure. Thus, this study aimed to evaluate the effects of F exposure on biochemical, proteomic, and genotoxic parameters of submandibular glands. Twenty one old rats (n = 30) were allocated into three groups: 60 days administration of drinking water containing 10 mgF/L, 50 mgF/L, or only deionized water (control). The submandibular glands were collected for oxidative biochemistry, protein expression profile, and genotoxic potential analyses. The results showed that both F concentrations increased the levels of thiobarbituric acid–reactive substances (TBARS) and reduced glutathione (GSH) and changed the proteomic profile, mainly regarding the cytoskeleton and cellular activity. Only the exposure to 50 mgF/L induced significant changes in DNA integrity. These findings reinforce the importance of continuous monitoring of F concentration in drinking water and the need for strategies to minimize F intake from other sources to obtain maximum preventive/therapeutic effects and avoid potential adverse effects. |
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Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicityfluoridegenotoxicityproteomesubmandibular glandtoxicityAlthough fluoride (F) is well-known to prevent dental caries, changes in cell processes in different tissues have been associated with its excessive exposure. Thus, this study aimed to evaluate the effects of F exposure on biochemical, proteomic, and genotoxic parameters of submandibular glands. Twenty one old rats (n = 30) were allocated into three groups: 60 days administration of drinking water containing 10 mgF/L, 50 mgF/L, or only deionized water (control). The submandibular glands were collected for oxidative biochemistry, protein expression profile, and genotoxic potential analyses. The results showed that both F concentrations increased the levels of thiobarbituric acid–reactive substances (TBARS) and reduced glutathione (GSH) and changed the proteomic profile, mainly regarding the cytoskeleton and cellular activity. Only the exposure to 50 mgF/L induced significant changes in DNA integrity. These findings reinforce the importance of continuous monitoring of F concentration in drinking water and the need for strategies to minimize F intake from other sources to obtain maximum preventive/therapeutic effects and avoid potential adverse effects.Laboratory of Functional and Structural Biology Institute of Biological Sciences Federal University of ParáLaboratory of Clinical Immunology and Oxidative Stress Faculty of Pharmacy Institute of Health Sciences Federal University of ParáSection of Parasitology Evandro Chagas InstituteLaboratory of Cell Culture and Cytogenetics Environment Section Evandro Chagas InstituteDepartment of Biological Sciences Bauru School of Dentistry University of São PauloDepartment of Chemistry University of Nebraska-LincolnDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP)Universidade Federal do Pará (UFPA)Evandro Chagas InstituteUniversidade de São Paulo (USP)University of Nebraska-LincolnUniversidade Estadual Paulista (UNESP)Lima, Leidiane Alencar de OliveiraMiranda, Giza Hellen NonatoAragão, Walessa Alana BragançaBittencourt, Leonardo Oliveirados Santos, Sávio Monteirode Souza, Michel Platini CaldasNogueira, Lygia S.de Oliveira, Edivaldo Herculano CorrêaMonteiro, Marta ChagasDionizio, AlineLeite, Aline LimaPessan, Juliano Pelim [UNESP]Buzalaf, Marília Afonso RabeloLima, Rafael Rodrigues2022-04-29T08:45:58Z2022-04-29T08:45:58Z2021-09-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphar.2021.715394Frontiers in Pharmacology, v. 12.1663-9812http://hdl.handle.net/11449/23152610.3389/fphar.2021.7153942-s2.0-85116868062Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Pharmacologyinfo:eu-repo/semantics/openAccess2024-04-23T15:22:56Zoai:repositorio.unesp.br:11449/231526Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:02:47.109847Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
title |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
spellingShingle |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity Lima, Leidiane Alencar de Oliveira fluoride genotoxicity proteome submandibular gland toxicity |
title_short |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
title_full |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
title_fullStr |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
title_full_unstemmed |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
title_sort |
Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity |
author |
Lima, Leidiane Alencar de Oliveira |
author_facet |
Lima, Leidiane Alencar de Oliveira Miranda, Giza Hellen Nonato Aragão, Walessa Alana Bragança Bittencourt, Leonardo Oliveira dos Santos, Sávio Monteiro de Souza, Michel Platini Caldas Nogueira, Lygia S. de Oliveira, Edivaldo Herculano Corrêa Monteiro, Marta Chagas Dionizio, Aline Leite, Aline Lima Pessan, Juliano Pelim [UNESP] Buzalaf, Marília Afonso Rabelo Lima, Rafael Rodrigues |
author_role |
author |
author2 |
Miranda, Giza Hellen Nonato Aragão, Walessa Alana Bragança Bittencourt, Leonardo Oliveira dos Santos, Sávio Monteiro de Souza, Michel Platini Caldas Nogueira, Lygia S. de Oliveira, Edivaldo Herculano Corrêa Monteiro, Marta Chagas Dionizio, Aline Leite, Aline Lima Pessan, Juliano Pelim [UNESP] Buzalaf, Marília Afonso Rabelo Lima, Rafael Rodrigues |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Pará (UFPA) Evandro Chagas Institute Universidade de São Paulo (USP) University of Nebraska-Lincoln Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Lima, Leidiane Alencar de Oliveira Miranda, Giza Hellen Nonato Aragão, Walessa Alana Bragança Bittencourt, Leonardo Oliveira dos Santos, Sávio Monteiro de Souza, Michel Platini Caldas Nogueira, Lygia S. de Oliveira, Edivaldo Herculano Corrêa Monteiro, Marta Chagas Dionizio, Aline Leite, Aline Lima Pessan, Juliano Pelim [UNESP] Buzalaf, Marília Afonso Rabelo Lima, Rafael Rodrigues |
dc.subject.por.fl_str_mv |
fluoride genotoxicity proteome submandibular gland toxicity |
topic |
fluoride genotoxicity proteome submandibular gland toxicity |
description |
Although fluoride (F) is well-known to prevent dental caries, changes in cell processes in different tissues have been associated with its excessive exposure. Thus, this study aimed to evaluate the effects of F exposure on biochemical, proteomic, and genotoxic parameters of submandibular glands. Twenty one old rats (n = 30) were allocated into three groups: 60 days administration of drinking water containing 10 mgF/L, 50 mgF/L, or only deionized water (control). The submandibular glands were collected for oxidative biochemistry, protein expression profile, and genotoxic potential analyses. The results showed that both F concentrations increased the levels of thiobarbituric acid–reactive substances (TBARS) and reduced glutathione (GSH) and changed the proteomic profile, mainly regarding the cytoskeleton and cellular activity. Only the exposure to 50 mgF/L induced significant changes in DNA integrity. These findings reinforce the importance of continuous monitoring of F concentration in drinking water and the need for strategies to minimize F intake from other sources to obtain maximum preventive/therapeutic effects and avoid potential adverse effects. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-27 2022-04-29T08:45:58Z 2022-04-29T08:45:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fphar.2021.715394 Frontiers in Pharmacology, v. 12. 1663-9812 http://hdl.handle.net/11449/231526 10.3389/fphar.2021.715394 2-s2.0-85116868062 |
url |
http://dx.doi.org/10.3389/fphar.2021.715394 http://hdl.handle.net/11449/231526 |
identifier_str_mv |
Frontiers in Pharmacology, v. 12. 1663-9812 10.3389/fphar.2021.715394 2-s2.0-85116868062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128308198506496 |