Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2180-7-92 http://hdl.handle.net/11449/18049 |
Resumo: | Background: Paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. The present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions.Methods: Soils samples of clayey, sandy and medium textures were collected from ground surface and the interior of armadillo burrows in a hyperendemic area of Paracoccidioidomycosis. P. brasiliensis was inoculated in soil with controlled humidity and in culture medium containing soil extracts. The molecular detection was carried out by Nested PCR, using panfungal and species specific primers from the ITS-5.8S rDNA region.Results: the soil texture does not affect fungus development and the growth is more abundant on/in soil saturated with water. Some soil samples inhibited the development of P. brasiliensis, especially those that contain high values of Exchangeable Aluminum (H+Al) in their composition. Some isolates produced a large number of conidia, mainly in soil-extract agar medium. The molecular detection was positive only in samples collected from armadillo burrows, both in sandy and clayey soil.Conclusion: P. brasiliensis may grow and produce the infectious conidia in sandy and clayey soil, containing high water content, mainly in wild animal burrows, but without high values of H+Al. |
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spelling |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detectionBackground: Paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. The present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions.Methods: Soils samples of clayey, sandy and medium textures were collected from ground surface and the interior of armadillo burrows in a hyperendemic area of Paracoccidioidomycosis. P. brasiliensis was inoculated in soil with controlled humidity and in culture medium containing soil extracts. The molecular detection was carried out by Nested PCR, using panfungal and species specific primers from the ITS-5.8S rDNA region.Results: the soil texture does not affect fungus development and the growth is more abundant on/in soil saturated with water. Some soil samples inhibited the development of P. brasiliensis, especially those that contain high values of Exchangeable Aluminum (H+Al) in their composition. Some isolates produced a large number of conidia, mainly in soil-extract agar medium. The molecular detection was positive only in samples collected from armadillo burrows, both in sandy and clayey soil.Conclusion: P. brasiliensis may grow and produce the infectious conidia in sandy and clayey soil, containing high water content, mainly in wild animal burrows, but without high values of H+Al.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Microbiol & Imunol, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Microbiol & Imunol, BR-18618000 São Paulo, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Tercarioli, Gisela Ramos [UNESP]Bagagli, Eduardo [UNESP]Reis, Gabriela Martins [UNESP]Theodoro, Raquel Cordeiro [UNESP]Gimenes Bosco, Sandra de Moraes [UNESP]da Graca Macoris, Severino Assis [UNESP]Richini-Pereira, Virginia Bodelao [UNESP]2014-05-20T13:50:34Z2014-05-20T13:50:34Z2007-10-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1471-2180-7-92Bmc Microbiology. London: Biomed Central Ltd., v. 7, 8 p., 2007.1471-2180http://hdl.handle.net/11449/1804910.1186/1471-2180-7-92WOS:000207212400001WOS:000252406900001WOS000207212400001.pdf33203275704295390000-0002-8003-4109Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Microbiology2.8291,242info:eu-repo/semantics/openAccess2023-11-10T06:10:28Zoai:repositorio.unesp.br:11449/18049Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:17:54.771870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
title |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
spellingShingle |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection Tercarioli, Gisela Ramos [UNESP] |
title_short |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
title_full |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
title_fullStr |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
title_full_unstemmed |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
title_sort |
Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection |
author |
Tercarioli, Gisela Ramos [UNESP] |
author_facet |
Tercarioli, Gisela Ramos [UNESP] Bagagli, Eduardo [UNESP] Reis, Gabriela Martins [UNESP] Theodoro, Raquel Cordeiro [UNESP] Gimenes Bosco, Sandra de Moraes [UNESP] da Graca Macoris, Severino Assis [UNESP] Richini-Pereira, Virginia Bodelao [UNESP] |
author_role |
author |
author2 |
Bagagli, Eduardo [UNESP] Reis, Gabriela Martins [UNESP] Theodoro, Raquel Cordeiro [UNESP] Gimenes Bosco, Sandra de Moraes [UNESP] da Graca Macoris, Severino Assis [UNESP] Richini-Pereira, Virginia Bodelao [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tercarioli, Gisela Ramos [UNESP] Bagagli, Eduardo [UNESP] Reis, Gabriela Martins [UNESP] Theodoro, Raquel Cordeiro [UNESP] Gimenes Bosco, Sandra de Moraes [UNESP] da Graca Macoris, Severino Assis [UNESP] Richini-Pereira, Virginia Bodelao [UNESP] |
description |
Background: Paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. The present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions.Methods: Soils samples of clayey, sandy and medium textures were collected from ground surface and the interior of armadillo burrows in a hyperendemic area of Paracoccidioidomycosis. P. brasiliensis was inoculated in soil with controlled humidity and in culture medium containing soil extracts. The molecular detection was carried out by Nested PCR, using panfungal and species specific primers from the ITS-5.8S rDNA region.Results: the soil texture does not affect fungus development and the growth is more abundant on/in soil saturated with water. Some soil samples inhibited the development of P. brasiliensis, especially those that contain high values of Exchangeable Aluminum (H+Al) in their composition. Some isolates produced a large number of conidia, mainly in soil-extract agar medium. The molecular detection was positive only in samples collected from armadillo burrows, both in sandy and clayey soil.Conclusion: P. brasiliensis may grow and produce the infectious conidia in sandy and clayey soil, containing high water content, mainly in wild animal burrows, but without high values of H+Al. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-22 2014-05-20T13:50:34Z 2014-05-20T13:50:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2180-7-92 Bmc Microbiology. London: Biomed Central Ltd., v. 7, 8 p., 2007. 1471-2180 http://hdl.handle.net/11449/18049 10.1186/1471-2180-7-92 WOS:000207212400001 WOS:000252406900001 WOS000207212400001.pdf 3320327570429539 0000-0002-8003-4109 |
url |
http://dx.doi.org/10.1186/1471-2180-7-92 http://hdl.handle.net/11449/18049 |
identifier_str_mv |
Bmc Microbiology. London: Biomed Central Ltd., v. 7, 8 p., 2007. 1471-2180 10.1186/1471-2180-7-92 WOS:000207212400001 WOS:000252406900001 WOS000207212400001.pdf 3320327570429539 0000-0002-8003-4109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Microbiology 2.829 1,242 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128787139788800 |