Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/srep35074 http://hdl.handle.net/11449/173626 |
Resumo: | Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair. |
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Mechanisms underlying heterologous skin scaffold-mediated tissue remodelingBiocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair.Post-Graduation in Structural and Functional Biology Federal University of São Paulo (UNIFESP)Department of Biology Instituto de Biociências Letras e Ciências Exatas São Paulo State University (UNESP)Department of Surgical Research Northwick Park Institute for Medical Research University College London (UCL)Department of Biology Instituto de Biociências Letras e Ciências Exatas São Paulo State University (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)University College London (UCL)Mimura, Kallyne K. O.Moraes, Andréia R. [UNESP]Miranda, Aline C. [UNESP]Greco, RebeccaAnsari, TaheraSibbons, PaulGreco, Karin V.Oliani, Sonia M. [UNESP]2018-12-11T17:06:57Z2018-12-11T17:06:57Z2016-10-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1038/srep35074Scientific Reports, v. 6.2045-2322http://hdl.handle.net/11449/17362610.1038/srep350742-s2.0-849915037242-s2.0-84991503724.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-11-08T06:13:13Zoai:repositorio.unesp.br:11449/173626Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:10:25.565764Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
title |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
spellingShingle |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling Mimura, Kallyne K. O. |
title_short |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
title_full |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
title_fullStr |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
title_full_unstemmed |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
title_sort |
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling |
author |
Mimura, Kallyne K. O. |
author_facet |
Mimura, Kallyne K. O. Moraes, Andréia R. [UNESP] Miranda, Aline C. [UNESP] Greco, Rebecca Ansari, Tahera Sibbons, Paul Greco, Karin V. Oliani, Sonia M. [UNESP] |
author_role |
author |
author2 |
Moraes, Andréia R. [UNESP] Miranda, Aline C. [UNESP] Greco, Rebecca Ansari, Tahera Sibbons, Paul Greco, Karin V. Oliani, Sonia M. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) University College London (UCL) |
dc.contributor.author.fl_str_mv |
Mimura, Kallyne K. O. Moraes, Andréia R. [UNESP] Miranda, Aline C. [UNESP] Greco, Rebecca Ansari, Tahera Sibbons, Paul Greco, Karin V. Oliani, Sonia M. [UNESP] |
description |
Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-11 2018-12-11T17:06:57Z 2018-12-11T17:06:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/srep35074 Scientific Reports, v. 6. 2045-2322 http://hdl.handle.net/11449/173626 10.1038/srep35074 2-s2.0-84991503724 2-s2.0-84991503724.pdf |
url |
http://dx.doi.org/10.1038/srep35074 http://hdl.handle.net/11449/173626 |
identifier_str_mv |
Scientific Reports, v. 6. 2045-2322 10.1038/srep35074 2-s2.0-84991503724 2-s2.0-84991503724.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128766812094464 |